FastQCFastQC Report
Thu 26 May 2016
SRR1926760_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926760_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792658
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15970.20147402789096935No Hit
GTCCTACAGTGGACATTTCTAAATT12690.16009426511812155No Hit
CTGTAGGACGTGGAATATGGCAAGA11920.15038011349156888No Hit
GTCCTACAGTGTGCATTTCTCATTT10380.1309518102384635No Hit
GGTATCAACGCAGAGTACTTTTTTT10170.12830249615849457No Hit
TATCAACGCAGAGTACTTTTTTTTT9610.12123765861191081No Hit
GTCCTAAAGTGTGTATTTCTCATTT9130.11518208357198187No Hit
CTTTAGGACGTGAAATATGGCGAGG8550.10786493039873439No Hit
CTGTAGGACCTGGAATATGGCGAGA7980.10067393503881876No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTC350.00219099916.2610578
GCGCCAC400.005331611314.22752813
GTAATAC551.9115009E-413.857583
TAGGACC3000.013.337924
TATTAGG751.4180025E-512.7164782
CTAGACA1001.3865429E-712.3852114
TAAGGTG1001.3865429E-712.3852115
AATCCCG853.9980278E-612.27705319
GTATTTA701.0503848E-412.2623191
GACTTAT701.0503848E-412.2623191
GATAGTA550.003098224512.0741267
TCCAACG658.10975E-411.67607918
GTATTAG600.00570224811.1269191
GTCCATA600.00570224811.1269191
TTGAGCT600.00574819211.1149354
GTAGGAC7150.011.0594153
AGGACCT5550.010.986195
CGTGGAA2950.010.9325759
GTCCTAC6550.010.920641
GGCGAGG2550.010.78892519
Produced by FastQC (version 0.11.5)