FastQCFastQC Report
Thu 26 May 2016
SRR1926759_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926759_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences698351
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12240.1752700289682409No Hit
GTCCTACAGTGTGCATTTCTCATTT10160.14548557960108885No Hit
CTGTAGGACGTGGAATATGGCAAGA9310.13331404981162767No Hit
GTCCTAAAGTGTGTATTTCTCATTT9270.13274127193918245No Hit
GTATCAACGCAGAGTACTTTTTTTT7600.10882779576459403No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTAG350.002111030316.351411
TAGGACC2850.013.7000864
AATCCCG500.001519290513.27523719
GTACTAA603.9504794E-412.7177651
CCTAGGA1206.9849193E-1012.7086092
AGTGCAT854.0109335E-612.27298410
GCAGTCC701.07761676E-412.2283228
ACTAGAC550.002990539712.1309443
CTAGACA951.0068743E-612.0293454
GTATTAG1052.573306E-711.8093521
CATACTG1052.6415E-711.7855825
GTATAGA1302.4429028E-911.7394741
TGGTACT657.8511116E-411.72094
TTATACT1156.812661E-811.5934984
AGGACCT4000.011.4229475
GTAGGAC6400.011.1696763
TAAGGGT600.0057643711.11043554
AGGACGT4300.011.06874755
CTCGAAC600.005952156611.06269618
CTATACT951.3208444E-511.0268984
Produced by FastQC (version 0.11.5)