##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926759_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 698351 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.79277326158336 31.0 30.0 33.0 23.0 34.0 2 28.79938741406542 31.0 30.0 31.0 23.0 34.0 3 28.50514855710094 31.0 28.0 33.0 16.0 34.0 4 32.44853376024378 35.0 33.0 37.0 25.0 37.0 5 32.49735305025696 35.0 33.0 37.0 25.0 37.0 6 32.009581141861325 35.0 32.0 37.0 18.0 37.0 7 31.87453300704087 35.0 32.0 37.0 17.0 37.0 8 31.58466158135379 35.0 32.0 37.0 17.0 37.0 9 33.822683722082445 37.0 34.0 39.0 23.0 39.0 10 33.99524451171402 38.0 34.0 39.0 25.0 39.0 11 34.14162792063017 38.0 35.0 39.0 25.0 39.0 12 34.01126367686164 38.0 35.0 39.0 25.0 39.0 13 34.1185621557068 38.0 35.0 39.0 25.0 39.0 14 35.207955598259325 39.0 36.0 41.0 26.0 41.0 15 34.772943691639306 39.0 34.0 41.0 19.0 41.0 16 34.86871644774619 39.0 34.0 41.0 23.0 41.0 17 35.10633191618541 39.0 36.0 41.0 25.0 41.0 18 35.16428271743006 39.0 36.0 41.0 25.0 41.0 19 34.53867754180921 39.0 34.0 41.0 18.0 41.0 20 34.82454381822321 39.0 34.0 41.0 22.0 41.0 21 34.80728315703708 39.0 35.0 41.0 22.0 41.0 22 34.83396315033558 39.0 35.0 41.0 22.0 41.0 23 34.84410131867786 39.0 35.0 41.0 22.0 41.0 24 33.53176697677815 39.0 33.0 41.0 8.0 41.0 25 34.12163224510311 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.4202540215912265 1101 2 -0.3718114297781234 1101 3 -1.2515633250665097 1101 4 -0.601008725543263 1101 5 -0.3254588486564458 1101 6 -2.1731134589158962 1101 7 -0.4487394041606514 1101 8 0.017491455042311088 1101 9 -0.22102065919931135 1101 10 -9.586516549894458 1101 11 -4.271793865970476 1101 12 -1.389300077794978 1101 13 -0.8638177577272259 1101 14 -0.5881852288389098 1101 15 -1.7377880094134213 1101 16 -0.0881975951582703 1101 17 -0.15799197608392745 1101 18 -0.18452440074801046 1101 19 -0.861058053490936 1101 20 0.014461534996343062 1101 21 0.006055057253092855 1101 22 0.09731880280840954 1101 23 0.12254382509869544 1101 24 -0.06623871485755473 1101 25 0.04061677178003009 1102 1 -0.20587471789643175 1102 2 -0.24785651151287524 1102 3 -0.33735078068244206 1102 4 -0.27433725651096097 1102 5 -0.10601437270041458 1102 6 -0.7725555147821588 1102 7 -0.21205367928704533 1102 8 -0.05116535732132377 1102 9 0.10487940621874259 1102 10 0.07386828219290464 1102 11 -0.12885731391774868 1102 12 -0.23156978167430964 1102 13 -0.22172540105136562 1102 14 -0.47999492659238285 1102 15 0.21933575170859143 1102 16 -0.04335030660416095 1102 17 -0.19297078139148738 1102 18 -0.3060261420782311 1102 19 0.13437686311049646 1102 20 -0.17785808323449714 1102 21 -0.21346882789852373 1102 22 -0.1806209445190916 1102 23 -0.6899011084055573 1102 24 -3.5730215836470975 1102 25 -1.8886475965576324 1103 1 -0.1883993776397439 1103 2 -0.47066130941444584 1103 3 -0.035296067465051095 1103 4 0.02556150849937211 1103 5 -0.27081702237426697 1103 6 0.30786309045252835 1103 7 0.15052392375019963 1103 8 0.2306047205495183 1103 9 -0.02576198354755377 1103 10 0.07162065310864563 1103 11 -0.26456045316749055 1103 12 -0.22087303595942132 1103 13 -0.27987906399193463 1103 14 -0.2673155577814512 1103 15 0.29549167868429294 1103 16 -0.14093350217491007 1103 17 -0.22367518969758038 1103 18 -0.47098802695985853 1103 19 -2.0193034485658785 1103 20 -0.6450869949438811 1103 21 -0.3706166068568919 1103 22 -0.3306851693862285 1103 23 -0.44237914601015405 1103 24 1.2059271358605699 1103 25 0.4889521594747279 1104 1 -0.2899699371214304 1104 2 -0.2833655833801778 1104 3 -1.7812240858991224 1104 4 -0.6686884505253197 1104 5 -0.4481610510666272 1104 6 -1.7999598190836288 1104 7 -0.4874780237821241 1104 8 0.2502140571351674 1104 9 0.1583019957816134 1104 10 0.1892814932470941 1104 11 -0.12406238623793797 1104 12 0.1501260866869103 1104 13 -0.0731128353593462 1104 14 -0.15842246959294215 1104 15 0.174601020090563 1104 16 -0.15392990598898848 1104 17 -0.12788166668874368 1104 18 -0.27583564701751584 1104 19 -1.2167812977960253 1104 20 -0.5931052361872133 1104 21 -0.08790625890421211 1104 22 -0.12695304185178458 1104 23 -0.2483412944661083 1104 24 1.118266970609561 1104 25 0.4770963154315737 1105 1 0.0955897102393557 1105 2 -0.05783271874453533 1105 3 0.3246774131895229 1105 4 0.13205551406196747 1105 5 0.36892223576892036 1105 6 -0.059333772883427116 1105 7 0.16217417277331947 1105 8 0.8974093106411019 1105 9 -0.151462539426511 1105 10 0.013651913215106504 1105 11 0.12984156793384471 1105 12 -0.08386751521764069 1105 13 -0.016683121413734625 1105 14 -0.024732033257713226 1105 15 0.36670011617358966 1105 16 -0.1635177496076352 1105 17 -0.2804929823901148 1105 18 -0.015371785097343604 1105 19 -0.7673411311479583 1105 20 0.08213376243644888 1105 21 -0.020995943835210085 1105 22 -0.16704901328354538 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0.7360066259161187 1110 8 0.9903522535840423 1110 9 0.4959124217415507 1110 10 0.580106298635009 1110 11 0.3252335116016454 1110 12 0.3034629186645148 1110 13 0.36813618064694964 1110 14 0.0792754224738843 1110 15 0.5515711941481172 1110 16 0.2299325972909898 1110 17 -0.11965110603099305 1110 18 0.03703061730506363 1110 19 -1.8080784201611095 1110 20 -0.06981301192411848 1110 21 -0.05969843943425701 1110 22 0.03605754154715157 1110 23 0.11850905102501486 1110 24 -1.2935584470411001 1110 25 -0.8012470765040547 1111 1 0.5778512821561002 1111 2 0.3255472129112569 1111 3 0.8443160173108843 1111 4 0.7457347104997822 1111 5 0.9775015046930164 1111 6 0.831641685333409 1111 7 0.8666372565467455 1111 8 1.2263882896200826 1111 9 0.5193358451649743 1111 10 0.6602864788151876 1111 11 0.28649477286290903 1111 12 0.6322917474933476 1111 13 0.45912717163793815 1111 14 0.5423384855369449 1111 15 -0.38536574278882085 1111 16 -0.03763497027657792 1111 17 0.23079934441945227 1111 18 0.46946304973749164 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-2.905504411676901 2216 11 -1.6076116841917525 2216 12 -1.4035504692253653 2216 13 -1.2416193916584248 2216 14 -1.3639498745362744 2216 15 -2.2152067391636834 2216 16 -1.1753974339354585 2216 17 -1.5113420001123288 2216 18 -1.1500552733664904 2216 19 -0.5250627351533907 2216 20 -1.3055331942578476 2216 21 -4.377249855392044 2216 22 -2.7146333884266483 2216 23 -2.508790758744915 2216 24 -2.7314680698112817 2216 25 -2.143732988711079 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31428.0 3 4.0 4 299.0 5 1647.0 6 2218.0 7 1734.0 8 1327.0 9 1160.0 10 1089.0 11 1103.0 12 1092.0 13 1063.0 14 1089.0 15 1165.0 16 1217.0 17 1339.0 18 1459.0 19 1892.0 20 2553.0 21 3150.0 22 4069.0 23 4919.0 24 5714.0 25 6787.0 26 7851.0 27 8576.0 28 9461.0 29 11618.0 30 13627.0 31 16745.0 32 21741.0 33 28472.0 34 39305.0 35 54759.0 36 81087.0 37 131176.0 38 194416.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.82477381280426 18.168314273904656 11.850285891430145 26.156626021860934 2 14.477231402825497 20.088791506300872 41.713509730929616 23.720467359944017 3 19.210003656726585 23.889896679548862 29.02064258007156 27.879457083652998 4 11.469795474443977 16.217745991668806 39.16020284899958 33.152255684887635 5 12.50513832293733 37.70576566382836 37.057370181255685 12.731725831978629 6 32.347978779476136 37.453880880903824 18.07797855031281 12.12016178930724 7 27.820745610002756 31.6604130274892 22.392146271949986 18.12669509055806 8 24.453672992419726 37.69853867491012 20.709359634216238 17.13842869845392 9 25.987977983984965 15.380561113346957 19.985992933449246 38.64546796921883 10 13.643089713893627 29.80273514998632 35.39135678852652 21.16281834759353 11 34.708564750499114 22.64971341985638 24.28942159791703 18.352300231727472 12 22.712814027529728 24.944036824700277 32.430140914058036 19.91300823371196 13 29.051065828745582 22.028368631809304 25.586907578526457 23.333657960918657 14 20.04978359318383 22.331722575341367 28.01215086319251 29.606342968282295 15 22.842939840341884 31.671397595675998 22.975558617212943 22.51010394676917 16 21.660357685570695 28.65322946788292 28.150659327910905 21.535753518635484 17 21.289165720210995 29.098320154564682 28.156781754721656 21.455732370502663 18 21.228651834241564 28.092574955781995 29.797850324742747 20.880922885233694 19 23.208538686626934 27.375330677884293 27.320474314188033 22.09565632130074 20 22.960298011964593 27.964830921748945 28.24826310068216 20.82660796560431 21 22.410346012832264 27.30665673693859 27.354204857928504 22.928792392300643 22 22.404762893655537 28.629207239437953 27.566664900298036 21.399364966608474 23 21.752045188936503 28.33288457572355 27.86226196750613 22.052808267833818 24 22.825135805934643 27.43988806125123 28.281147286244394 21.453828846569728 25 22.189839353921197 27.735616397156864 28.217969961746842 21.856574287175096 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 209.0 1 209.0 2 292.0 3 375.0 4 375.0 5 375.0 6 531.0 7 687.0 8 687.0 9 687.0 10 1025.0 11 1363.0 12 1363.0 13 1363.0 14 2360.0 15 3357.0 16 3357.0 17 3357.0 18 5690.5 19 8024.0 20 8024.0 21 8024.0 22 12747.5 23 17471.0 24 17471.0 25 17471.0 26 24996.0 27 32521.0 28 32521.0 29 32521.0 30 42581.5 31 52642.0 32 52642.0 33 52642.0 34 60690.5 35 68739.0 36 68739.0 37 68739.0 38 74830.0 39 80921.0 40 80921.0 41 80921.0 42 85013.5 43 89106.0 44 89106.0 45 89106.0 46 91453.5 47 93801.0 48 93801.0 49 93801.0 50 89441.0 51 85081.0 52 85081.0 53 85081.0 54 75135.0 55 65189.0 56 65189.0 57 65189.0 58 55474.5 59 45760.0 60 45760.0 61 45760.0 62 36815.5 63 27871.0 64 27871.0 65 27871.0 66 21333.5 67 14796.0 68 14796.0 69 14796.0 70 10748.5 71 6701.0 72 6701.0 73 6701.0 74 4630.5 75 2560.0 76 2560.0 77 2560.0 78 1689.0 79 818.0 80 818.0 81 818.0 82 538.5 83 259.0 84 259.0 85 259.0 86 168.0 87 77.0 88 77.0 89 77.0 90 47.0 91 17.0 92 17.0 93 17.0 94 10.5 95 4.0 96 4.0 97 4.0 98 3.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22667684302020044 2 0.14133294002586094 3 0.14405363491997578 4 0.0704516783107635 5 0.023770281706477117 6 0.02276792042969796 7 0.31832130261143754 8 0.31531421878110005 9 0.018901669790692646 10 0.03637139490027221 11 0.016753752769023026 12 0.01775611404580218 13 0.01646736383280041 14 0.01847208638635872 15 0.015608197024132565 16 0.020047225535583107 17 0.018901669790692646 18 0.01517861361979864 19 0.0234838927702545 20 0.01775611404580218 21 0.01589458596035518 22 0.01718333617335695 23 0.018185697450136106 24 0.016037780428466486 25 0.015465002556021255 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 698351.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.7928019242384 #Duplication Level Percentage of deduplicated Percentage of total 1 93.18256851398655 80.87576211826209 2 5.3180644351490685 9.231394262804598 3 0.8029158657545739 2.0906195309479534 4 0.2586812515099881 0.8980668249528198 5 0.12065190977587711 0.5235858653479394 6 0.07227650551440996 0.37638482561329856 7 0.04326103626337136 0.262832258601086 8 0.030782455005954338 0.21373564160588604 9 0.023210911279184027 0.18130860226217613 >10 0.12446800539976073 1.9634280113195741 >50 0.011724971775557251 0.6882649395212033 >100 0.009907946599011253 1.6419091392676979 >500 0.0011559271007223083 0.7316642934398523 >1k 3.302648859206595E-4 0.32104368605392153 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1224 0.1752700289682409 No Hit GTCCTACAGTGTGCATTTCTCATTT 1016 0.14548557960108885 No Hit CTGTAGGACGTGGAATATGGCAAGA 931 0.13331404981162767 No Hit GTCCTAAAGTGTGTATTTCTCATTT 927 0.13274127193918245 No Hit GTATCAACGCAGAGTACTTTTTTTT 760 0.10882779576459403 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4319446811130793E-4 2 0.0 0.0 0.0 0.0 1.4319446811130793E-4 3 0.0 0.0 0.0 0.0 1.4319446811130793E-4 4 0.0 0.0 0.0 0.0 1.4319446811130793E-4 5 0.0 0.0 0.0 0.0 1.4319446811130793E-4 6 0.0 0.0 0.0 0.0 1.4319446811130793E-4 7 0.0 0.0 0.0 0.0 1.4319446811130793E-4 8 0.0 0.0 0.0 0.0 1.4319446811130793E-4 9 0.0 0.0 0.0 0.0 1.4319446811130793E-4 10 0.0 0.0 0.0 0.0 1.4319446811130793E-4 11 0.0 0.0 0.0 0.0 1.4319446811130793E-4 12 0.0 0.0 0.0 0.0 1.4319446811130793E-4 13 0.0 0.0 0.0 0.0 1.4319446811130793E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACTAG 35 0.0021110303 16.35141 1 TAGGACC 285 0.0 13.700086 4 AATCCCG 50 0.0015192905 13.275237 19 GTACTAA 60 3.9504794E-4 12.717765 1 CCTAGGA 120 6.9849193E-10 12.708609 2 AGTGCAT 85 4.0109335E-6 12.272984 10 GCAGTCC 70 1.07761676E-4 12.228322 8 ACTAGAC 55 0.0029905397 12.130944 3 CTAGACA 95 1.0068743E-6 12.029345 4 GTATTAG 105 2.573306E-7 11.809352 1 CATACTG 105 2.6415E-7 11.785582 5 GTATAGA 130 2.4429028E-9 11.739474 1 TGGTACT 65 7.8511116E-4 11.7209 4 TTATACT 115 6.812661E-8 11.593498 4 AGGACCT 400 0.0 11.422947 5 GTAGGAC 640 0.0 11.169676 3 TAAGGGT 60 0.00576437 11.1104355 4 AGGACGT 430 0.0 11.0687475 5 CTCGAAC 60 0.0059521566 11.062696 18 CTATACT 95 1.3208444E-5 11.026898 4 >>END_MODULE