FastQCFastQC Report
Thu 26 May 2016
SRR1926759_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926759_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences698351
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12860.184148085991142No Hit
GTCCTACAGTGGACATTTCTAAATT11710.1676807221583416No Hit
CTGTAGGACGTGGAATATGGCAAGA10810.15479322002832388No Hit
GTCCTACAGTGTGCATTTCTCATTT9650.13818266172741214No Hit
GTCCTAAAGTGTGTATTTCTCATTT9410.13474599449274077No Hit
CTTTAGGACGTGAAATATGGCGAGG8150.11670349151071595No Hit
CTGTAGGACCTGGAATATGGCGAGA8050.11527154682960288No Hit
GGTATCAACGCAGAGTACTTTTTTT7640.10940057363703926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAAG555.7052057E-717.327652
TAGGACC2750.014.5426744
TAGGACT1204.9112714E-1113.4894844
CTAGACT604.0080422E-412.6959854
TGTAGGA6050.012.6019272
GTAGGAC5550.012.3537393
TCGAACT550.003091619112.07723819
GTAAGAC951.007591E-612.028643
GGACCTG4700.011.750546
AGGACCT4600.011.3849875
AGGACTA855.1891788E-511.202345
CTGTAGG6300.011.1950171
CGTTGAT1658.185452E-1110.9254614
CCATGTA2100.010.8916652
ATAGACC700.001460277610.8830563
TAGACTG1053.3622018E-610.8822745
TATACTA1053.3622018E-610.8822745
GTCCTAA2900.010.8454991
GTTGATA1752.0008883E-1110.84486715
GATTAAA1158.428178E-710.7740521
Produced by FastQC (version 0.11.5)