##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926759_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 698351 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.949102958254517 31.0 30.0 33.0 26.0 34.0 2 30.230746429803922 31.0 30.0 33.0 26.0 34.0 3 30.271788828253985 31.0 30.0 34.0 26.0 34.0 4 34.005114906400934 35.0 35.0 37.0 30.0 37.0 5 34.04047248446698 37.0 35.0 37.0 30.0 37.0 6 34.03022548832894 37.0 35.0 37.0 30.0 37.0 7 34.020870593727224 37.0 35.0 37.0 30.0 37.0 8 34.04478406990181 37.0 35.0 37.0 30.0 37.0 9 35.59904116984153 39.0 35.0 39.0 30.0 39.0 10 35.48486935652702 39.0 35.0 39.0 30.0 39.0 11 35.553291969224645 39.0 35.0 39.0 30.0 39.0 12 35.44865404359699 39.0 35.0 39.0 30.0 39.0 13 35.45796454791358 39.0 35.0 39.0 30.0 39.0 14 36.56233183599651 40.0 37.0 41.0 30.0 41.0 15 36.55875913401714 40.0 37.0 41.0 30.0 41.0 16 36.53579646911081 40.0 37.0 41.0 30.0 41.0 17 36.52171902095078 40.0 37.0 41.0 30.0 41.0 18 36.49779695310811 40.0 37.0 41.0 30.0 41.0 19 36.57374157121562 40.0 37.0 41.0 30.0 41.0 20 36.54263973274185 40.0 37.0 41.0 30.0 41.0 21 36.500633635521396 40.0 37.0 41.0 30.0 41.0 22 36.40350053196745 40.0 37.0 41.0 30.0 41.0 23 36.345489589046196 40.0 37.0 41.0 30.0 41.0 24 36.27795048621682 40.0 37.0 41.0 30.0 41.0 25 36.15852486786731 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.39632753856907854 1101 2 -0.47480364587617174 1101 3 -0.1273805124027234 1101 4 -0.23090093164950076 1101 5 -0.11772887676197286 1101 6 -0.07807333572895203 1101 7 -0.21181581203392597 1101 8 -0.16304157168548272 1101 9 -0.298070610199197 1101 10 -0.19879048365593377 1101 11 -0.2580876566939452 1101 12 -0.18761702828776805 1101 13 -0.17307079768499278 1101 14 -0.243198063599948 1101 15 -0.35582158902415273 1101 16 -0.22970151606658362 1101 17 -0.02539979661123226 1101 18 -0.10994222638473161 1101 19 -0.20466375036755835 1101 20 -0.11995706476694323 1101 21 -0.23033838606263402 1101 22 -0.2696390623314713 1101 23 -0.27985326191544146 1101 24 -0.16594249588133891 1101 25 -0.11992587186687587 1102 1 -0.7807351648769689 1102 2 -0.6631178264678113 1102 3 -0.44727478473088667 1102 4 -0.5543175690103936 1102 5 -0.588993146783487 1102 6 -0.4200896864604573 1102 7 -0.5352532470412754 1102 8 -0.3981069708301348 1102 9 -0.5928290360820583 1102 10 -0.5683646612980908 1102 11 -0.5441035082819354 1102 12 -0.44930436025224196 1102 13 -0.4192270144796595 1102 14 -0.5700595474931163 1102 15 -0.3852209529943096 1102 16 -0.41177451202428017 1102 17 -0.5225013609723845 1102 18 -0.4767153391873862 1102 19 -0.5626489121921523 1102 20 -0.6004573805130278 1102 21 -0.49202571802710793 1102 22 -0.6517324777966067 1102 23 -0.5248419744654953 1102 24 -0.5762119955655933 1102 25 -0.6156207590490936 1103 1 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2214 9 0.32426020411647727 2214 10 0.25288449457309525 2214 11 0.2069573040204702 2214 12 0.1488593693756428 2214 13 0.2337747442408329 2214 14 0.2436610790642959 2214 15 0.18100069792207307 2214 16 0.14643962502558594 2214 17 0.3355481455024574 2214 18 0.3120418832561356 2214 19 0.07990397772204716 2214 20 -0.02251038324926924 2214 21 0.1514020518354755 2214 22 0.20082988591070716 2214 23 0.10706328434500989 2214 24 0.05400133991827971 2214 25 0.055634547048910576 2215 1 0.00756109019886253 2215 2 0.11753938010183518 2215 3 0.06821824116945407 2215 4 0.15180246050704937 2215 5 -0.06360048823815134 2215 6 -0.07347915131575888 2215 7 -0.43142606115803517 2215 8 -0.3324742209904201 2215 9 -0.44598461959584057 2215 10 -0.18356533552154275 2215 11 -0.529394791363039 2215 12 -0.43898535416729345 2215 13 -0.3366975474468461 2215 14 -0.1612583506658325 2215 15 0.007170645020117661 2215 16 -0.16040771752935967 2215 17 -0.030876329216944498 2215 18 -0.14520144139353164 2215 19 -0.2850882139353743 2215 20 -0.32573632433715005 2215 21 -0.27755733434879915 2215 22 -0.4138880572779513 2215 23 -0.15664096554977647 2215 24 -0.2519298701487429 2215 25 -0.2115415458581893 2216 1 0.26814530933889813 2216 2 0.3451236488360294 2216 3 -1.7344433117888371 2216 4 -1.287178544931777 2216 5 -0.35145157352356904 2216 6 0.015787020511460526 2216 7 -0.05762169356616198 2216 8 0.06302924233470009 2216 9 -0.09134343330367045 2216 10 0.04961976639444288 2216 11 0.08737795168964624 2216 12 0.14010244401755756 2216 13 0.1425406863399985 2216 14 0.020319074075032972 2216 15 -0.006173488817978523 2216 16 0.07455616139477428 2216 17 -0.08872625730542438 2216 18 -0.025451395932222454 2216 19 -0.05745104476971363 2216 20 -0.06633575235123601 2216 21 0.10933327747775223 2216 22 0.0916945913805236 2216 23 -0.09116676277581348 2216 24 -0.25756045982323883 2216 25 -0.2194468540858594 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14574.0 3 3.0 4 210.0 5 1523.0 6 2076.0 7 1634.0 8 1202.0 9 1027.0 10 956.0 11 852.0 12 788.0 13 761.0 14 795.0 15 807.0 16 835.0 17 865.0 18 945.0 19 1094.0 20 1447.0 21 1892.0 22 2504.0 23 2994.0 24 3737.0 25 4257.0 26 5163.0 27 5752.0 28 6773.0 29 8176.0 30 10742.0 31 13778.0 32 18126.0 33 24082.0 34 32965.0 35 46668.0 36 69745.0 37 122723.0 38 285880.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.340924241446785 19.064003039230442 9.921510440186655 28.67356227913611 2 14.655650700396972 20.659524352252866 39.75768888353669 24.927136063813467 3 18.93202979649185 24.05661390741661 29.17246130423387 27.83889499185767 4 11.94456655750475 17.00190878225992 37.952261828221054 33.10126283201427 5 12.661211560547954 38.68089347649884 35.74435849040392 12.913536472549284 6 32.179688689790325 38.182912707283926 17.29159542890639 12.34580317401936 7 28.478391021252335 31.61109853877642 21.485372009017706 18.425138430953535 8 25.161325431991273 38.50128458865953 19.455575000214814 16.881814979134386 9 26.122622000469732 15.56461914772955 19.54771465724155 38.765044194559174 10 13.829441423741532 30.13393400531841 34.7107165820122 21.325907988927856 11 35.02445171591617 22.55472100699541 24.0614908744621 18.359336402626322 12 22.894602690922007 25.217799721628758 31.89870952590972 19.98888806153952 13 29.141183798838682 21.985265162634516 25.491555033686787 23.381996004840016 14 20.273186406262752 22.515468582417725 27.53028205014384 29.681062961175687 15 22.630672274322635 31.705308800554466 22.964324940465193 22.699693984657703 16 21.805921697952453 28.832084909078155 27.811532089348134 21.550461303621265 17 21.287953856816372 29.21752478706891 27.87535727173272 21.619164084381996 18 21.230849420130678 28.202312031124926 29.638703968084634 20.92813458065976 19 23.03235636101199 27.43904185305893 27.519660684930724 22.008941100998356 20 23.051467324220372 27.91094958659461 28.19075358060331 20.846829508581706 21 22.3996024340887 27.244006078060977 27.21292833931735 23.143463148532973 22 22.481914460985067 28.62083087515107 27.21637349859955 21.68088116526431 23 21.72477983819002 28.260614305147847 27.81327414620176 22.20133171046037 24 22.498525090642488 27.32248105529049 28.58818239616924 21.590811457897782 25 22.164177073980355 27.868831330632517 28.049697339422348 21.917294255964784 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 181.0 1 181.0 2 218.5 3 256.0 4 256.0 5 256.0 6 412.0 7 568.0 8 568.0 9 568.0 10 937.0 11 1306.0 12 1306.0 13 1306.0 14 2153.5 15 3001.0 16 3001.0 17 3001.0 18 5247.0 19 7493.0 20 7493.0 21 7493.0 22 11984.5 23 16476.0 24 16476.0 25 16476.0 26 24068.0 27 31660.0 28 31660.0 29 31660.0 30 41529.0 31 51398.0 32 51398.0 33 51398.0 34 59299.5 35 67201.0 36 67201.0 37 67201.0 38 73705.5 39 80210.0 40 80210.0 41 80210.0 42 84451.5 43 88693.0 44 88693.0 45 88693.0 46 91563.0 47 94433.0 48 94433.0 49 94433.0 50 90709.0 51 86985.0 52 86985.0 53 86985.0 54 76623.0 55 66261.0 56 66261.0 57 66261.0 58 56651.5 59 47042.0 60 47042.0 61 47042.0 62 37981.5 63 28921.0 64 28921.0 65 28921.0 66 22162.5 67 15404.0 68 15404.0 69 15404.0 70 11194.0 71 6984.0 72 6984.0 73 6984.0 74 4795.0 75 2606.0 76 2606.0 77 2606.0 78 1746.5 79 887.0 80 887.0 81 887.0 82 580.5 83 274.0 84 274.0 85 274.0 86 184.0 87 94.0 88 94.0 89 94.0 90 54.0 91 14.0 92 14.0 93 14.0 94 8.5 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1154147412977142 2 0.11713307491504987 3 0.021192781280473572 4 0.0 5 0.006443751065008856 6 0.3329271383587909 7 0.024199865110811042 8 0.010453196172125478 9 0.010739585108348094 10 0.0031502782984487742 11 0.005155000852007085 12 4.295834043339238E-4 13 8.591668086678476E-4 14 0.0 15 0.0028638893622261585 16 5.727778724452317E-4 17 4.295834043339238E-4 18 5.727778724452317E-4 19 4.295834043339238E-4 20 7.159723405565396E-4 21 0.014605835747353408 22 4.295834043339238E-4 23 1.4319446811130793E-4 24 4.295834043339238E-4 25 0.0063005565968975484 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 698351.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.31828135367783 #Duplication Level Percentage of deduplicated Percentage of total 1 91.53211843017061 77.17830914693286 2 6.6230093794031735 11.168815365211278 3 1.0060521381344538 2.544857617190701 4 0.3082555986534223 1.039663291844226 5 0.1509516089004666 0.6363990115029942 6 0.08399720732353463 0.42495000960174006 7 0.05208598912482826 0.3074260759928311 8 0.0420614777036073 0.28372412089313653 9 0.03291251211535209 0.24976138109387286 >10 0.1430564507518413 2.2169438381762276 >50 0.012409900606916177 0.7119632539050313 >100 0.011049415098290483 1.7561219291874604 >500 0.0015299190101779848 0.9739510686913835 >1k 5.099730033926616E-4 0.507113889776323 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1286 0.184148085991142 No Hit GTCCTACAGTGGACATTTCTAAATT 1171 0.1676807221583416 No Hit CTGTAGGACGTGGAATATGGCAAGA 1081 0.15479322002832388 No Hit GTCCTACAGTGTGCATTTCTCATTT 965 0.13818266172741214 No Hit GTCCTAAAGTGTGTATTTCTCATTT 941 0.13474599449274077 No Hit CTTTAGGACGTGAAATATGGCGAGG 815 0.11670349151071595 No Hit CTGTAGGACCTGGAATATGGCGAGA 805 0.11527154682960288 No Hit GGTATCAACGCAGAGTACTTTTTTT 764 0.10940057363703926 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 2.8638893622261587E-4 13 0.0 0.0 0.0 0.0 2.8638893622261587E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTTAAG 55 5.7052057E-7 17.32765 2 TAGGACC 275 0.0 14.542674 4 TAGGACT 120 4.9112714E-11 13.489484 4 CTAGACT 60 4.0080422E-4 12.695985 4 TGTAGGA 605 0.0 12.601927 2 GTAGGAC 555 0.0 12.353739 3 TCGAACT 55 0.0030916191 12.077238 19 GTAAGAC 95 1.007591E-6 12.02864 3 GGACCTG 470 0.0 11.75054 6 AGGACCT 460 0.0 11.384987 5 AGGACTA 85 5.1891788E-5 11.20234 5 CTGTAGG 630 0.0 11.195017 1 CGTTGAT 165 8.185452E-11 10.92546 14 CCATGTA 210 0.0 10.891665 2 ATAGACC 70 0.0014602776 10.883056 3 TAGACTG 105 3.3622018E-6 10.882274 5 TATACTA 105 3.3622018E-6 10.882274 5 GTCCTAA 290 0.0 10.845499 1 GTTGATA 175 2.0008883E-11 10.844867 15 GATTAAA 115 8.428178E-7 10.774052 1 >>END_MODULE