FastQCFastQC Report
Thu 26 May 2016
SRR1926758_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926758_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844806
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT21000.24857777998735803No Hit
GTCCTACAGTGTGCATTTCTCATTT15890.18809052019043426No Hit
CTGTAGGACGTGGAATATGGCAAGA15190.17980459419085565No Hit
GTCCTAAAGTGTGTATTTCTCATTT14460.1711635570770094No Hit
CTTTAGGACGTGAAATATGGCGAGG10880.12878696410773596No Hit
CTGTAGGACCTGGAATATGGCGAGA10530.12464400110794667No Hit
GTATCAACGCAGAGTACTTTTTTTT9580.1133988158228043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4400.014.937794
CGGTTTC400.005351221714.2198113
AGGACCT6150.013.4639525
GGACCTG5800.013.2918516
ATAGGAC1352.5465852E-1112.7204033
TAGGACA3700.012.3574674
GGATTAG550.002948250612.1545111
CCATAAC550.002970719712.1422023
CTGTAGG9800.012.0836391
TCCAACG1103.915011E-812.06600818
TGTAGGA10050.012.05590252
GTCCTAT1052.5352892E-711.8237761
GTCCTAC11500.011.7921411
ACGTTTT907.6220786E-611.58719819
CCAACGA907.6220786E-611.58719819
GTAGGAC10400.011.5584423
TATGTCG752.1117239E-411.37652216
TATACTG2100.011.33050255
CTATACT1451.1605152E-911.1678834
GTCCTAA4200.011.1416351
Produced by FastQC (version 0.11.5)