##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926758_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 844806 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.743336339940768 31.0 30.0 33.0 23.0 34.0 2 28.742144350300542 31.0 30.0 31.0 23.0 34.0 3 28.454112541814332 31.0 28.0 33.0 16.0 34.0 4 32.37867747151417 35.0 33.0 37.0 25.0 37.0 5 32.41983721706522 35.0 33.0 37.0 25.0 37.0 6 31.959272306304644 35.0 32.0 37.0 18.0 37.0 7 31.842022902299462 35.0 32.0 37.0 17.0 37.0 8 31.55491083159921 35.0 32.0 37.0 17.0 37.0 9 33.77291354464812 37.0 34.0 39.0 23.0 39.0 10 33.93231345421316 38.0 34.0 39.0 24.0 39.0 11 34.07801317698975 38.0 35.0 39.0 25.0 39.0 12 33.95560992701283 38.0 34.0 39.0 24.0 39.0 13 34.069505898395605 38.0 35.0 39.0 25.0 39.0 14 35.12658409149556 39.0 36.0 41.0 25.0 41.0 15 34.72338619754121 39.0 34.0 41.0 19.0 41.0 16 34.80611880123958 39.0 34.0 41.0 22.0 41.0 17 35.03092662694157 39.0 35.0 41.0 25.0 41.0 18 35.07695612957294 39.0 36.0 41.0 25.0 41.0 19 34.479146691666486 39.0 34.0 41.0 18.0 41.0 20 34.75966079786365 39.0 34.0 41.0 19.0 41.0 21 34.74479939773155 39.0 35.0 41.0 19.0 41.0 22 34.76654995348044 39.0 35.0 41.0 19.0 41.0 23 34.77149546759848 39.0 35.0 41.0 19.0 41.0 24 33.510285201572906 39.0 33.0 41.0 2.0 41.0 25 34.07491779177705 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.2682545845282398 1101 2 -0.3209957236637635 1101 3 -1.2785378310117892 1101 4 -0.36926971271373077 1101 5 -0.36597001159823606 1101 6 -1.9642641471166726 1101 7 -0.515575130543759 1101 8 -0.3051272576076265 1101 9 -0.32786278496346455 1101 10 -9.235809570127032 1101 11 -3.998778833444934 1101 12 -1.6149173152268474 1101 13 -0.8685213674459078 1101 14 -0.7765664086328528 1101 15 -1.804875860783099 1101 16 -0.42974905098776617 1101 17 -0.4062321367651336 1101 18 -0.1627877230166206 1101 19 -0.8453799827916839 1101 20 -0.26746710820015096 1101 21 -0.36707604584520226 1101 22 -0.22735375990422568 1101 23 -0.3758241788605474 1101 24 -0.48843191706961164 1101 25 -0.5116195466001514 1102 1 0.041136161972030294 1102 2 -0.145720157878241 1102 3 -0.3581331609824012 1102 4 0.0264883742534181 1102 5 0.03373586960792352 1102 6 -0.6704962683201288 1102 7 0.1588543400814011 1102 8 0.5458202779940002 1102 9 0.32520115825588647 1102 10 0.3060818879509668 1102 11 0.14415991108501203 1102 12 0.20260361295643747 1102 13 0.1693085739252851 1102 14 -0.07961079158773288 1102 15 0.3861655380942324 1102 16 0.23968442916668664 1102 17 -0.023389023082955873 1102 18 -0.20347957152009855 1102 19 0.34122234078578373 1102 20 0.16259292426598648 1102 21 0.10441665577173609 1102 22 0.002964779977268961 1102 23 -0.2791659298226392 1102 24 -3.568739474609931 1102 25 -1.8251790338482863 1103 1 -0.12721859594123686 1103 2 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-0.04424645309557107 2215 3 0.2904468511989968 2215 4 0.36015205997558297 2215 5 0.5353495993863291 2215 6 0.8531800041056599 2215 7 0.35808125997011686 2215 8 -0.7245112458965828 2215 9 -0.21508989607903573 2215 10 -0.27425009676731804 2215 11 -0.40513171900692413 2215 12 -0.6562332024021131 2215 13 -0.19995377417507854 2215 14 -0.3031860705689411 2215 15 -0.31446857192810285 2215 16 0.08927470892241729 2215 17 -0.4144568497029226 2215 18 -1.1843713230928827 2215 19 0.14023095049081036 2215 20 -3.962272505716797 2215 21 -2.1080276909550335 2215 22 -1.0869349317000498 2215 23 -0.8411314321498224 2215 24 -0.8034773330492158 2215 25 -0.6084017962938262 2216 1 -0.47072180728163815 2216 2 -1.0195989129200775 2216 3 -0.04552869844114937 2216 4 -0.28925146955216974 2216 5 -0.4875233178965246 2216 6 -1.9526170973436123 2216 7 -9.956490365781633 2216 8 -11.281338614525193 2216 9 -5.949635888389 2216 10 -3.4039060171064683 2216 11 -2.2859756507825324 2216 12 -1.8037043647801099 2216 13 -1.5748822963085658 2216 14 -1.6664612357074589 2216 15 -2.6664058685858976 2216 16 -1.761647109080144 2216 17 -2.091298020954028 2216 18 -1.8195975875116943 2216 19 -1.0560423414346545 2216 20 -2.1422981391585765 2216 21 -4.592085661969524 2216 22 -3.174926650126551 2216 23 -3.043862379601265 2216 24 -3.26105398689916 2216 25 -2.590399232949647 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38951.0 3 6.0 4 324.0 5 2048.0 6 2600.0 7 2151.0 8 1634.0 9 1488.0 10 1494.0 11 1355.0 12 1390.0 13 1367.0 14 1411.0 15 1418.0 16 1452.0 17 1595.0 18 1851.0 19 2273.0 20 2956.0 21 4041.0 22 5029.0 23 6033.0 24 7108.0 25 8163.0 26 9401.0 27 10208.0 28 11536.0 29 13597.0 30 16467.0 31 20324.0 32 26362.0 33 34556.0 34 47466.0 35 66213.0 36 97361.0 37 158834.0 38 234343.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.35388780981513 18.619935192293315 12.178201022542687 25.84797597534887 2 13.826195804726321 20.270834123582294 42.99290198384099 22.910068087850398 3 18.98176136862045 24.290476224344886 29.151938017577177 27.575824389457487 4 11.108847324717784 16.61186197747006 40.087655914997455 32.1916347828147 5 11.96134634259539 38.30865385867641 37.50415863059403 12.225841168134174 6 31.39962895039372 38.25942926556093 18.5645565933526 11.77638519069274 7 27.463473423613294 31.74442973420645 22.741645343418092 18.05045149876216 8 23.675433982739236 38.88302501493012 20.90751397732521 16.534027025005432 9 25.69058026264071 15.663373791810118 20.501697740607966 38.14434820494121 10 13.202781701773436 30.31808639000425 36.15158451142011 20.327547396802203 11 34.33192571467132 22.811472624835886 24.84298955000006 18.013612110492737 12 22.505093699203595 25.249357442128357 33.05204038021905 19.193508478449004 13 28.7766428473337 22.60954288663797 26.01575485502408 22.598059411004247 14 19.393521089076444 23.087942283399965 28.585533676106543 28.93300295141705 15 22.163003021205263 32.69673349938913 23.279380984401488 21.86088249500412 16 20.816378719409183 29.227958804579846 29.301124598505666 20.65453787750531 17 20.639094361781726 29.658538779917222 28.924637488989706 20.77772936931135 18 20.326957864131 28.83865911523199 30.900465966454206 19.933917054182807 19 22.824830930686318 27.6124050465059 27.932192312792438 21.630571710015346 20 22.27103807281178 28.607943211025354 29.23244671594029 19.88857200022257 21 21.854423021828023 27.778225014028752 27.814095992158105 22.55325597198512 22 21.618610708260576 29.537159262438216 28.28910527717761 20.5551247521236 23 21.138302822078916 28.97162293511835 28.55074402630168 21.339330216501057 24 22.327850026933117 27.78515067747148 29.36940989836448 20.51758939723092 25 21.57130392677998 28.455273965385995 29.0266098566688 20.946812251165227 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 195.0 1 195.0 2 274.0 3 353.0 4 353.0 5 353.0 6 548.5 7 744.0 8 744.0 9 744.0 10 1200.5 11 1657.0 12 1657.0 13 1657.0 14 2952.5 15 4248.0 16 4248.0 17 4248.0 18 7507.0 19 10766.0 20 10766.0 21 10766.0 22 17175.5 23 23585.0 24 23585.0 25 23585.0 26 33743.5 27 43902.0 28 43902.0 29 43902.0 30 58067.0 31 72232.0 32 72232.0 33 72232.0 34 82678.5 35 93125.0 36 93125.0 37 93125.0 38 99214.5 39 105304.0 40 105304.0 41 105304.0 42 108853.5 43 112403.0 44 112403.0 45 112403.0 46 112482.5 47 112562.0 48 112562.0 49 112562.0 50 105261.0 51 97960.0 52 97960.0 53 97960.0 54 84184.5 55 70409.0 56 70409.0 57 70409.0 58 58398.5 59 46388.0 60 46388.0 61 46388.0 62 36744.0 63 27100.0 64 27100.0 65 27100.0 66 20166.0 67 13232.0 68 13232.0 69 13232.0 70 9433.0 71 5634.0 72 5634.0 73 5634.0 74 3848.5 75 2063.0 76 2063.0 77 2063.0 78 1358.5 79 654.0 80 654.0 81 654.0 82 428.5 83 203.0 84 203.0 85 203.0 86 129.0 87 55.0 88 55.0 89 55.0 90 38.5 91 22.0 92 22.0 93 22.0 94 13.5 95 5.0 96 5.0 97 5.0 98 5.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23733259470221563 2 0.14086074199283624 3 0.14346489016413236 4 0.07054874136784066 5 0.020951555741791607 6 0.020714814998946503 7 0.30906503978428185 8 0.30172607675608365 9 0.01822903719907292 10 0.033972296598272264 11 0.01384933345643852 12 0.015980000142044443 13 0.013494222342170866 14 0.015033037170664036 15 0.01302074085648066 16 0.017637185341960165 17 0.016808592742002304 18 0.01302074085648066 19 0.021543407598904364 20 0.015980000142044443 21 0.013257481599325762 22 0.015506518656354239 23 0.01716370385626996 24 0.014322814942128727 25 0.014086074199283623 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 844806.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.91895330098748 #Duplication Level Percentage of deduplicated Percentage of total 1 94.33267955791997 81.99297769251862 2 4.419149010251509 7.682156129043119 3 0.607143385551096 1.5831680272715742 4 0.20791407576038187 0.7228669536653843 5 0.09861135022082418 0.4285597672395569 6 0.07206330110902692 0.37582000222863066 7 0.039272599042786974 0.2389473241545885 8 0.03677158039378088 0.25569178232404377 9 0.022380604694269706 0.17507688608421806 >10 0.13830959294095765 2.25221428884448 >50 0.01319422266240599 0.8007255466012074 >100 0.010742900559289104 1.937969061872312 >500 9.519024812372492E-4 0.5142780516887802 >1k 8.159164124890707E-4 1.0395484864635307 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2100 0.24857777998735803 No Hit GTCCTACAGTGTGCATTTCTCATTT 1589 0.18809052019043426 No Hit CTGTAGGACGTGGAATATGGCAAGA 1519 0.17980459419085565 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1446 0.1711635570770094 No Hit CTTTAGGACGTGAAATATGGCGAGG 1088 0.12878696410773596 No Hit CTGTAGGACCTGGAATATGGCGAGA 1053 0.12464400110794667 No Hit GTATCAACGCAGAGTACTTTTTTTT 958 0.1133988158228043 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1837037142255145E-4 2 0.0 0.0 0.0 0.0 1.1837037142255145E-4 3 0.0 0.0 0.0 0.0 1.1837037142255145E-4 4 0.0 0.0 0.0 0.0 1.1837037142255145E-4 5 0.0 1.1837037142255145E-4 0.0 0.0 1.1837037142255145E-4 6 0.0 1.1837037142255145E-4 0.0 0.0 1.1837037142255145E-4 7 0.0 1.1837037142255145E-4 0.0 0.0 1.1837037142255145E-4 8 0.0 1.1837037142255145E-4 0.0 0.0 1.1837037142255145E-4 9 0.0 1.1837037142255145E-4 0.0 2.367407428451029E-4 1.1837037142255145E-4 10 0.0 1.1837037142255145E-4 0.0 2.367407428451029E-4 1.1837037142255145E-4 11 0.0 1.1837037142255145E-4 0.0 2.367407428451029E-4 1.1837037142255145E-4 12 0.0 1.1837037142255145E-4 0.0 2.367407428451029E-4 1.1837037142255145E-4 13 0.0 1.1837037142255145E-4 0.0 2.367407428451029E-4 1.1837037142255145E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 440 0.0 14.93779 4 CGGTTTC 40 0.0053512217 14.21981 13 AGGACCT 615 0.0 13.463952 5 GGACCTG 580 0.0 13.291851 6 ATAGGAC 135 2.5465852E-11 12.720403 3 TAGGACA 370 0.0 12.357467 4 GGATTAG 55 0.0029482506 12.154511 1 CCATAAC 55 0.0029707197 12.142202 3 CTGTAGG 980 0.0 12.083639 1 TCCAACG 110 3.915011E-8 12.066008 18 TGTAGGA 1005 0.0 12.0559025 2 GTCCTAT 105 2.5352892E-7 11.823776 1 GTCCTAC 1150 0.0 11.792141 1 ACGTTTT 90 7.6220786E-6 11.587198 19 CCAACGA 90 7.6220786E-6 11.587198 19 GTAGGAC 1040 0.0 11.558442 3 TATGTCG 75 2.1117239E-4 11.376522 16 TATACTG 210 0.0 11.3305025 5 CTATACT 145 1.1605152E-9 11.167883 4 GTCCTAA 420 0.0 11.141635 1 >>END_MODULE