FastQCFastQC Report
Thu 26 May 2016
SRR1926758_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926758_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844806
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT20720.2452634095875266No Hit
CTGTAGGACGTGGAATATGGCAAGA19010.2250220760742703No Hit
GTCCTACAGTGTGCATTTCTCATTT16420.19436414987582948No Hit
GTATCAACGCAGAGTACTTTTTTTT14680.1737677052483055No Hit
GTCCTAAAGTGTGTATTTCTCATTT13430.1589714088204866No Hit
CTTTAGGACGTGAAATATGGCGAGG13370.1582611865919513No Hit
CTGTAGGACCTGGAATATGGCGAGA12950.15328963099220413No Hit
GGTATCAACGCAGAGTACTTTTTTT9010.10665170465171886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4600.013.4601124
GGCGAGG2900.013.08434519
AACTGCC604.1350874E-412.6496967
GGACCTG6950.012.472396
GTCTAGG701.0486272E-412.2647161
TGTAGGA13000.012.2520462
GGACAGC1404.9112714E-1112.2472226
GTCCTAC10600.012.1490121
GTATTAA1103.5919584E-812.1408311
TGGCGAG9200.012.06390818
CTGTAGG13200.011.9962971
AGGACCT7400.011.9713835
GACGTGG5250.011.7461487
GGACTAC657.837634E-411.7238366
TCCTACA11500.011.693832
GTATTAG1156.663322E-811.6129681
TAGCACT1156.796654E-811.5964034
GGCGAGA6800.011.57868319
CCTACAG11200.011.5681953
GTAGGAC12850.011.4172723
Produced by FastQC (version 0.11.5)