FastQCFastQC Report
Thu 26 May 2016
SRR1926757_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926757_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743050
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17860.24036067559383623No Hit
GTCCTACAGTGTGCATTTCTCATTT15300.20590808155574997No Hit
CTGTAGGACGTGGAATATGGCAAGA14050.18908552587309063No Hit
GTCCTAAAGTGTGTATTTCTCATTT11660.15692079940784603No Hit
CTGTAGGACCTGGAATATGGCGAGA10900.14669268555278916No Hit
CTTTAGGACGTGAAATATGGCGAGG9180.12354484893344997No Hit
GTATCAACGCAGAGTACTTTTTTTT7450.10026243186864948No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTGC402.7387188E-416.6408278
CTAGGAC951.6370905E-1116.05753
TAGGACC3000.015.2370964
AAGGCGT759.463147E-713.9579046
TCCAACG903.72238E-813.69553818
AGGACCT4850.013.3430755
TTACCCC500.001470982313.3324594
GTCCTAT1650.013.3080261
CTTATAC655.264717E-513.2011183
AAAGGCG801.9562867E-613.0855345
TAGGACA2000.012.8562994
ATAGGAC751.4226453E-512.7121883
CTAGTAC751.4226453E-512.7121883
GGACCTG4750.012.6222196
TGTAGGA8650.012.5652272
GTAGGAC8100.012.4767773
GTCCTAC9700.012.4013391
CTGTAGG8400.012.3884511
CCTAGAC701.0535272E-412.2581823
TAGGACT1251.3515091E-912.1896784
Produced by FastQC (version 0.11.5)