##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926757_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 743050 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.720925913464775 31.0 30.0 33.0 23.0 34.0 2 28.7481838368885 31.0 30.0 31.0 23.0 34.0 3 28.4568925375143 31.0 28.0 33.0 16.0 34.0 4 32.367408653522645 35.0 33.0 37.0 25.0 37.0 5 32.41486306439674 35.0 33.0 37.0 25.0 37.0 6 31.938017630038356 35.0 32.0 37.0 18.0 37.0 7 31.826626741134515 35.0 32.0 37.0 17.0 37.0 8 31.548232285848865 35.0 32.0 37.0 17.0 37.0 9 33.735861651302066 37.0 34.0 39.0 22.0 39.0 10 33.86794428369558 38.0 34.0 39.0 23.0 39.0 11 34.022186932238746 38.0 35.0 39.0 25.0 39.0 12 33.86949465042729 38.0 34.0 39.0 23.0 39.0 13 34.00352735347554 38.0 35.0 39.0 25.0 39.0 14 35.06159746988762 39.0 35.0 41.0 25.0 41.0 15 34.65447143530045 39.0 34.0 41.0 19.0 41.0 16 34.744835475405424 39.0 34.0 41.0 21.0 41.0 17 34.97958010901016 39.0 35.0 41.0 25.0 41.0 18 35.033096023147834 39.0 36.0 41.0 25.0 41.0 19 34.42913666644237 39.0 34.0 41.0 18.0 41.0 20 34.70067424803176 39.0 34.0 41.0 19.0 41.0 21 34.69408653522643 39.0 34.0 41.0 18.0 41.0 22 34.71738644774914 39.0 35.0 41.0 18.0 41.0 23 34.720678285445125 39.0 35.0 41.0 18.0 41.0 24 33.452879348630645 39.0 33.0 41.0 2.0 41.0 25 34.02486373729897 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.2940283618645019 1101 2 -0.4562971934142439 1101 3 -1.4342884442897343 1101 4 -0.7927377226187815 1101 5 -0.4126642109122898 1101 6 -2.615124211221687 1101 7 -0.7037350401984277 1101 8 -0.18927911662023433 1101 9 -0.3834759212654575 1101 10 -9.446735913567323 1101 11 -4.241719891077651 1101 12 -1.7943983924186213 1101 13 -0.8450895033309891 1101 14 -0.785740688373771 1101 15 -2.114421490493605 1101 16 -0.6220390913436518 1101 17 -0.2463600187767554 1101 18 -0.18224812048048022 1101 19 -0.7474862486482365 1101 20 -0.1784859791750506 1101 21 -0.41941260536111713 1101 22 -0.2662695724133002 1101 23 -0.3058631508452976 1101 24 -0.1845035134158124 1101 25 -0.4394253354097657 1102 1 -0.06344358404649597 1102 2 -0.014014824253052893 1102 3 -0.1584821034521191 1102 4 -0.16292318588691757 1102 5 0.005526502612127615 1102 6 -0.43271850512920196 1102 7 0.16791688273337257 1102 8 0.7515045723122533 1102 9 0.5155948545972677 1102 10 0.6165639301629398 1102 11 0.2755566911004763 1102 12 0.4905560451586979 1102 13 0.1630902806386061 1102 14 0.00502150605020546 1102 15 0.4090779002833429 1102 16 0.4635582312969788 1102 17 0.378734205178219 1102 18 0.2682106410118763 1102 19 0.37203678524016937 1102 20 0.47617824438140843 1102 21 0.17470832859435603 1102 22 0.19904456537709336 1102 23 -0.10898023613906815 1102 24 -3.1561002374322555 1102 25 -1.5216513694567269 1103 1 -0.10199330954034025 1103 2 -0.36478666286720696 1103 3 0.0026289789479605474 1103 4 -0.20250478579182385 1103 5 -0.4060449611508261 1103 6 0.5733366510559321 1103 7 0.1697609921775225 1103 8 0.21461118612765873 1103 9 0.1357255357205105 1103 10 0.19807425665902656 1103 11 0.1995270130831912 1103 12 0.0070548384341151404 1103 13 0.042370449183835035 1103 14 -0.09041137301721136 1103 15 0.22546196428367438 1103 16 0.09546761239627699 1103 17 0.11782588007059758 1103 18 -0.1531903309139011 1103 19 -1.6997697874036177 1103 20 -0.3221764413447943 1103 21 -0.1386714132475504 1103 22 -0.034884292136382555 1103 23 -0.2609732696947944 1103 24 1.290912408627932 1103 25 0.17825922439647002 1104 1 -0.2600415631126758 1104 2 -0.12749705454200821 1104 3 -1.609063678406013 1104 4 -0.4049663619979498 1104 5 -0.18028802817119072 1104 6 -1.5809395027653217 1104 7 -0.3517232796658156 1104 8 0.10916787921946991 1104 9 0.2205688163909727 1104 10 0.030038627864989564 1104 11 0.12000453978519232 1104 12 0.15326203813562245 1104 13 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1108 22 0.6086002164341267 1108 23 0.5864218534008501 1108 24 -0.13293789220737295 1108 25 0.34990885757780177 1109 1 0.3430591235025915 1109 2 0.28687091583014634 1109 3 -1.037491100127724 1109 4 0.2171252279237592 1109 5 0.5963174329755319 1109 6 0.9171183613783 1109 7 0.8182541096934912 1109 8 1.3187620225235044 1109 9 0.7898474594707778 1109 10 0.8265475574636412 1109 11 0.4453864514291652 1109 12 0.09176172155534346 1109 13 0.16742520798227645 1109 14 0.3922330747193996 1109 15 -0.8159117925920398 1109 16 0.4233505536167854 1109 17 0.29746420915862615 1109 18 0.43128636613095495 1109 19 -1.0710057671599884 1109 20 0.08977937567241412 1109 21 0.268090481956456 1109 22 0.5660351777825241 1109 23 0.5695197745439415 1109 24 -0.33342630420976604 1109 25 0.15878978339021899 1110 1 0.46412419084333223 1110 2 0.5915173578653636 1110 3 0.9707572411554821 1110 4 0.69737797646237 1110 5 0.7703843979653229 1110 6 0.8824519253463734 1110 7 0.8826717000498547 1110 8 1.2114943391743402 1110 9 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-3.320385879446331 2216 16 -2.268576232976926 2216 17 -2.4433346367643267 2216 18 -2.182912489917797 2216 19 -1.4479415847747887 2216 20 -2.0566816278722726 2216 21 -4.564023921059189 2216 22 -3.3332151850034926 2216 23 -3.1747632636401484 2216 24 -3.580954526561591 2216 25 -3.0715443216510465 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35341.0 3 1.0 4 323.0 5 1803.0 6 2411.0 7 1949.0 8 1531.0 9 1326.0 10 1214.0 11 1156.0 12 1161.0 13 1128.0 14 1188.0 15 1236.0 16 1290.0 17 1428.0 18 1719.0 19 2097.0 20 2724.0 21 3384.0 22 4501.0 23 5284.0 24 6378.0 25 7224.0 26 8209.0 27 9107.0 28 10171.0 29 11852.0 30 14488.0 31 17641.0 32 22840.0 33 30115.0 34 40721.0 35 57431.0 36 83958.0 37 138071.0 38 210649.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.4602776316322 18.48678619126634 12.10544066264654 25.94749551445492 2 13.89762622873053 20.21074733230372 42.963319344605175 22.928307094360576 3 19.322402574649196 23.897445296790853 29.075988808685665 27.704163319874286 4 11.1204784716059 16.532912430592123 39.85853789269404 32.488071205107936 5 12.051048730781346 38.120845661180944 37.449555659054134 12.378549948983576 6 31.677040371299658 38.1033080990276 18.4155691717907 11.804082357882042 7 27.7231639028236 31.48027150640697 22.456112404084475 18.340452186684956 8 23.70949213904184 38.90212509769672 20.791886751447397 16.596496011814047 9 25.684926897160686 15.694160656646828 20.306598503754074 38.31431394243841 10 13.253741952681363 30.261014493924293 36.01725377426312 20.467989779131226 11 34.443246502750554 22.81688586307661 24.6817715000249 18.058096134147934 12 22.54514520766257 25.152503041984776 33.097467345774064 19.20488440457859 13 28.971532404603273 22.529106938555756 25.850999394306477 22.648361262534493 14 19.432947159149798 23.078652199604537 28.442152614852834 29.046248026392828 15 22.210003647625882 32.77681240569676 23.118814346610602 21.89436960006676 16 20.71114078385396 29.254064036590798 29.347613939070072 20.687181240485167 17 20.489389252765417 29.613985780548802 29.02940535127321 20.867219615412573 18 20.25896762904637 28.854297059021466 30.895215021199274 19.99152029073289 19 22.97622541035464 27.62507167913592 27.74756560155285 21.651137308956596 20 22.318940848043027 28.730277521287224 29.03757265113725 19.913208979532502 21 21.814102236232515 27.846976765471513 27.723818420181278 22.615102578114694 22 21.579247741393605 29.627720288154023 28.309706354248547 20.483325616203818 23 21.156828117933365 29.030134817909676 28.463592703465206 21.34944436069175 24 22.389590531658722 27.71465759638409 29.36014935277324 20.535602519183943 25 21.503370375156138 28.469440496188135 29.0763503036568 20.950838824998925 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 154.0 1 154.0 2 218.0 3 282.0 4 282.0 5 282.0 6 479.5 7 677.0 8 677.0 9 677.0 10 1049.5 11 1422.0 12 1422.0 13 1422.0 14 2564.0 15 3706.0 16 3706.0 17 3706.0 18 6398.0 19 9090.0 20 9090.0 21 9090.0 22 14404.0 23 19718.0 24 19718.0 25 19718.0 26 28927.5 27 38137.0 28 38137.0 29 38137.0 30 50344.0 31 62551.0 32 62551.0 33 62551.0 34 71506.0 35 80461.0 36 80461.0 37 80461.0 38 86953.5 39 93446.0 40 93446.0 41 93446.0 42 96086.5 43 98727.0 44 98727.0 45 98727.0 46 99287.5 47 99848.0 48 99848.0 49 99848.0 50 93686.5 51 87525.0 52 87525.0 53 87525.0 54 75006.0 55 62487.0 56 62487.0 57 62487.0 58 51908.0 59 41329.0 60 41329.0 61 41329.0 62 32624.0 63 23919.0 64 23919.0 65 23919.0 66 17905.5 67 11892.0 68 11892.0 69 11892.0 70 8438.0 71 4984.0 72 4984.0 73 4984.0 74 3416.0 75 1848.0 76 1848.0 77 1848.0 78 1225.5 79 603.0 80 603.0 81 603.0 82 387.5 83 172.0 84 172.0 85 172.0 86 114.0 87 56.0 88 56.0 89 56.0 90 33.5 91 11.0 92 11.0 93 11.0 94 6.5 95 2.0 96 2.0 97 2.0 98 2.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23955319292106855 2 0.13781037615234507 3 0.1407711459524931 4 0.06984725119440145 5 0.020456227710113722 6 0.019648745037346073 7 0.30872754188816365 8 0.301056456496871 9 0.016687975237198036 10 0.034452594038086266 11 0.014669268555278918 12 0.01534217078258529 13 0.013458044546127448 14 0.016284233900814214 15 0.013592624991588722 16 0.017630038355426956 17 0.016687975237198036 18 0.013458044546127448 19 0.02099454949195882 20 0.015611331673507839 21 0.013996366327972545 22 0.01534217078258529 23 0.016418814346275488 24 0.014938429446201466 25 0.014265527218895095 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 743050.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.07870254469816 #Duplication Level Percentage of deduplicated Percentage of total 1 94.45439172157822 82.24965880763709 2 4.309346639118735 7.505046282996303 3 0.5795246031281148 1.5139275159938208 4 0.20853638440875372 0.7263631115070678 5 0.11203922533889255 0.48781151883119167 6 0.0744356384721005 0.3889055292742038 7 0.04229702125130261 0.25782188124482697 8 0.03119238684436163 0.21729540605434577 9 0.025180284946128985 0.19733998885332363 >10 0.13647815344711503 2.1984577653703563 >50 0.014216656172306476 0.8564716111576807 >100 0.010970640711764085 2.0806902474162827 >500 6.180642587461629E-4 0.3814509066383911 >1k 7.725803234327036E-4 0.9387594270250635 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1786 0.24036067559383623 No Hit GTCCTACAGTGTGCATTTCTCATTT 1530 0.20590808155574997 No Hit CTGTAGGACGTGGAATATGGCAAGA 1405 0.18908552587309063 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1166 0.15692079940784603 No Hit CTGTAGGACCTGGAATATGGCGAGA 1090 0.14669268555278916 No Hit CTTTAGGACGTGAAATATGGCGAGG 918 0.12354484893344997 No Hit GTATCAACGCAGAGTACTTTTTTTT 745 0.10026243186864948 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.3458044546127448E-4 0.0 11 0.0 0.0 0.0 1.3458044546127448E-4 0.0 12 0.0 0.0 0.0 1.3458044546127448E-4 0.0 13 0.0 0.0 0.0 1.3458044546127448E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGTGC 40 2.7387188E-4 16.640827 8 CTAGGAC 95 1.6370905E-11 16.0575 3 TAGGACC 300 0.0 15.237096 4 AAGGCGT 75 9.463147E-7 13.957904 6 TCCAACG 90 3.72238E-8 13.695538 18 AGGACCT 485 0.0 13.343075 5 TTACCCC 50 0.0014709823 13.332459 4 GTCCTAT 165 0.0 13.308026 1 CTTATAC 65 5.264717E-5 13.201118 3 AAAGGCG 80 1.9562867E-6 13.085534 5 TAGGACA 200 0.0 12.856299 4 ATAGGAC 75 1.4226453E-5 12.712188 3 CTAGTAC 75 1.4226453E-5 12.712188 3 GGACCTG 475 0.0 12.622219 6 TGTAGGA 865 0.0 12.565227 2 GTAGGAC 810 0.0 12.476777 3 GTCCTAC 970 0.0 12.401339 1 CTGTAGG 840 0.0 12.388451 1 CCTAGAC 70 1.0535272E-4 12.258182 3 TAGGACT 125 1.3515091E-9 12.189678 4 >>END_MODULE