FastQCFastQC Report
Thu 26 May 2016
SRR1926757_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926757_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743050
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17980.2419756409393715No Hit
CTGTAGGACGTGGAATATGGCAAGA16620.2236727003566382No Hit
GTCCTACAGTGTGCATTTCTCATTT15020.20213982908283426No Hit
GTATCAACGCAGAGTACTTTTTTTT13000.17495457909965684No Hit
CTGTAGGACCTGGAATATGGCGAGA12340.1660722696992127No Hit
CTTTAGGACGTGAAATATGGCGAGG11890.16001614965345537No Hit
GTCCTAAAGTGTGTATTTCTCATTT11890.16001614965345537No Hit
CACACACACACACACACACACACAC8190.11022138483278379No Hit
GGTATCAACGCAGAGTACTTTTTTT8060.10847183904178723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGT607.7961886E-817.39161919
GTAAGGC453.4486795E-516.9375533
TCCAACG1500.013.91329618
CGGTTTC852.7424358E-713.39157713
GTAAGAC751.4330759E-512.7031653
CCGGTTT751.4991632E-512.64759912
TAGGACC4550.012.5627214
GGCGAGG2950.012.54126119
CTAGGAC1301.8735591E-1012.4588743
GCCGGTT701.1041555E-412.19672111
TGTAGGA10250.012.0941252
TTTCCGT951.0555214E-611.98274317
CCAACGA951.0555214E-611.98274319
TTCCGTG1052.763427E-711.7449918
GTCCATA657.7572296E-411.73791
CTGTAGG10300.011.5740821
GTCCTAC8700.011.5102251
TGGCGAG9100.011.46700218
ACAGTCC1001.9601248E-611.382848
AGGACCT7400.011.3290845
Produced by FastQC (version 0.11.5)