FastQCFastQC Report
Thu 26 May 2016
SRR1926756_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926756_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746748
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17960.24050951592772932No Hit
CTGTAGGACGTGGAATATGGCAAGA13590.18198910475823168No Hit
GTCCTAAAGTGTGTATTTCTCATTT13240.1773021153052971No Hit
GTCCTACAGTGTGCATTTCTCATTT11200.1499836624939069No Hit
CTTTAGGACGTGAAATATGGCGAGG9390.1257452313230166No Hit
GTATCAACGCAGAGTACTTTTTTTT9080.12159389780756025No Hit
CTGTAGGACCTGGAATATGGCGAGA7620.10204245608960454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCCG402.793149E-416.5979779
TAGGAGT755.6759745E-815.2334634
CTATACC1006.366463E-1014.2813734
TCTAGCA954.660251E-914.0468812
TAGGACC4150.013.9945984
GTCTAGC500.001448428713.3598271
GCCTACG500.001448428713.3598271
GAACTGT500.00147812813.3239046
TCTATAC957.0513124E-813.0435323
GTATTAG905.106558E-712.7236441
GTATTAA701.0447137E-412.2692291
CTAGGAC1103.62852E-812.1313973
CTAGCAC959.962841E-712.0401833
GTAGGAC7700.012.0076083
TGTAGGA8150.011.9293842
TTTATAC802.7653918E-511.9147643
CTTACAC802.7653918E-511.9147643
TCAATAC657.7931065E-411.7314613
AGGACGT5450.011.5252655
CTAGAAC1001.8490482E-611.4381743
Produced by FastQC (version 0.11.5)