##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926756_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 746748 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.77507807185289 31.0 30.0 33.0 23.0 34.0 2 28.786729927632884 31.0 30.0 31.0 23.0 34.0 3 28.481521209296844 31.0 28.0 33.0 16.0 34.0 4 32.414216040752706 35.0 33.0 37.0 25.0 37.0 5 32.45912945197041 35.0 33.0 37.0 25.0 37.0 6 31.99131433897379 35.0 32.0 37.0 18.0 37.0 7 31.86590121433201 35.0 32.0 37.0 17.0 37.0 8 31.580719332358438 35.0 32.0 37.0 17.0 37.0 9 33.806435370432865 37.0 34.0 39.0 23.0 39.0 10 33.967661111914595 38.0 34.0 39.0 25.0 39.0 11 34.11538028893281 38.0 35.0 39.0 25.0 39.0 12 34.0028349590491 38.0 35.0 39.0 25.0 39.0 13 34.10693165565894 38.0 35.0 39.0 25.0 39.0 14 35.17099208836181 39.0 36.0 41.0 25.0 41.0 15 34.76506532324157 39.0 34.0 41.0 19.0 41.0 16 34.858416761745595 39.0 34.0 41.0 23.0 41.0 17 35.08839394280266 39.0 36.0 41.0 25.0 41.0 18 35.14121095737786 39.0 36.0 41.0 25.0 41.0 19 34.517964561003176 39.0 34.0 41.0 18.0 41.0 20 34.805002490800106 39.0 34.0 41.0 21.0 41.0 21 34.78839313931875 39.0 35.0 41.0 20.0 41.0 22 34.816278048284026 39.0 35.0 41.0 21.0 41.0 23 34.81675746034807 39.0 35.0 41.0 21.0 41.0 24 33.539106900855444 39.0 33.0 41.0 8.0 41.0 25 34.11148607026735 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.5495721860079748 1101 2 -0.6539390279194208 1101 3 -1.3909155041045587 1101 4 -1.0817203218916838 1101 5 -0.7040532798629684 1101 6 -2.9013799329214365 1101 7 -1.1484027426316814 1101 8 -0.5381996416324739 1101 9 -0.5981789897721796 1101 10 -9.347612691135748 1101 11 -3.9496295122829572 1101 12 -1.8347470901984053 1101 13 -0.9941996388310343 1101 14 -0.84466524826653 1101 15 -2.225190430065517 1101 16 -0.6700184020340103 1101 17 -0.6262664691867599 1101 18 -0.6316008967379005 1101 19 -1.1600946244832642 1101 20 -0.5441151400001303 1101 21 -0.5825606230795728 1101 22 -0.45599253464170886 1101 23 -0.4248160530932381 1101 24 -0.6710247335263517 1101 25 -0.4439789544634891 1102 1 -0.07409066325233837 1102 2 -0.1470880612895762 1102 3 -0.5039751897910065 1102 4 -0.3172518298212097 1102 5 0.06408752583026 1102 6 -0.5696709548152015 1102 7 0.1486212821849442 1102 8 0.3567728404540027 1102 9 0.4644572556700126 1102 10 0.2742351310551925 1102 11 -0.008140514560636802 1102 12 0.16603983414933055 1102 13 0.06592920170191974 1102 14 -0.22080856951088634 1102 15 0.3421250011462149 1102 16 0.2613888402767799 1102 17 0.07238875367738018 1102 18 -0.15334477322824824 1102 19 0.36419901723317594 1102 20 0.14973707462981878 1102 21 0.19563098240902121 1102 22 -0.09887062490827958 1102 23 -0.17462461704591448 1102 24 -3.664849902723237 1102 25 -1.725704387923578 1103 1 0.41301046938256647 1103 2 0.06054842502813429 1103 3 0.43235687676893164 1103 4 0.19201378222822285 1103 5 0.09238069060649678 1103 6 0.9772450764372778 1103 7 0.6089141809549936 1103 8 0.6486481354532465 1103 9 0.5995559618094077 1103 10 0.49036840664571457 1103 11 0.4032745572853642 1103 12 0.3113205761119673 1103 13 0.3170155288214147 1103 14 0.4129049580394124 1103 15 0.7136091372829938 1103 16 0.6898023566353473 1103 17 0.29779331827877087 1103 18 0.3029215378984276 1103 19 -0.9730230346802031 1103 20 0.11353184760813662 1103 21 0.37815745732414996 1103 22 0.4667323413851676 1103 23 0.5084630660563718 1103 24 1.9922032352577475 1103 25 1.2005745909370376 1104 1 -0.09669774945011511 1104 2 -0.022888637116594168 1104 3 -1.156877828018871 1104 4 -0.3051624003600182 1104 5 -0.02982222209852381 1104 6 -1.800912508372992 1104 7 -0.5933729595448831 1104 8 0.076556611533384 1104 9 0.1485750064191791 1104 10 -0.023975692142407468 1104 11 -0.0551174227221054 1104 12 -0.020780778107557296 1104 13 -0.11140201634849234 1104 14 -0.1307768402670959 1104 15 0.2498017154067469 1104 16 -0.14191041349841527 1104 17 -0.24552064170425325 1104 18 -0.2695825075252145 1104 19 -1.3493711102713775 1104 20 -0.5140210780230987 1104 21 -0.12069195078329642 1104 22 -0.07273806717868325 1104 23 -0.02537965553231203 1104 24 1.128865944271297 1104 25 0.7211344392695551 1105 1 -0.09397124340292606 1105 2 -0.3219465789639102 1105 3 0.3280041990832707 1105 4 2.898215583968522E-5 1105 5 0.06966889399600262 1105 6 -0.24206022845809372 1105 7 0.19488916837568482 1105 8 0.5793262433352702 1105 9 -0.5266593101813655 1105 10 0.14672083671351288 1105 11 -0.184523437327357 1105 12 -0.11796706220086861 1105 13 -0.2022248557481845 1105 14 -0.26748069091781446 1105 15 0.059851932952220466 1105 16 -0.6109604968748741 1105 17 -0.45587848963325683 1105 18 -0.29383355691857815 1105 19 -0.9912308844840894 1105 20 -0.4774216875337629 1105 21 -0.49957566458162006 1105 22 -0.36635258043266816 1105 23 -0.578596836832979 1105 24 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10 -0.16723054321150244 1107 11 -0.41409962516927123 1107 12 -0.16674234033720126 1107 13 -0.3987212599524952 1107 14 -0.23060380850341033 1107 15 -1.6389110518655272 1107 16 -0.7301380738617809 1107 17 -0.5238175147730928 1107 18 -0.930103456728034 1107 19 0.2596327211462466 1107 20 0.1418465526776842 1107 21 -0.16907286785163933 1107 22 -0.23008882728250768 1107 23 -0.5844638231259296 1107 24 -4.511747082548624 1107 25 -2.4048388644011887 1108 1 0.6470180692211756 1108 2 0.20153799612658574 1108 3 0.9279806090560712 1108 4 0.8443582364454016 1108 5 0.9711602211459365 1108 6 -2.4976980106205957 1108 7 -0.8927079452048261 1108 8 0.7301774371312497 1108 9 0.47251269548649333 1108 10 0.7360356479698567 1108 11 0.7434746442253939 1108 12 0.7985187736281674 1108 13 0.694385210475744 1108 14 0.6732630354628668 1108 15 -0.3764167184012379 1108 16 0.6593085700380072 1108 17 0.44955127848146503 1108 18 0.5527601837567246 1108 19 0.8365525434436876 1108 20 -0.4641214427286471 1108 21 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1.0269742717364387 1110 9 0.8214593362882567 1110 10 0.8888832931757449 1110 11 0.5778964792889596 1110 12 0.503431516249151 1110 13 0.5674885221857195 1110 14 0.43907589139559633 1110 15 1.0484392583350228 1110 16 0.7273139532627297 1110 17 0.29162037297759014 1110 18 0.3341044497789696 1110 19 -2.161388776473146 1110 20 -0.22321260184547498 1110 21 -0.09565633344102054 1110 22 0.1997301021649065 1110 23 0.1285862417997663 1110 24 -0.47738696135399294 1110 25 -0.19691792947116227 1111 1 0.2548744443806825 1111 2 0.24760426202325192 1111 3 0.6602355904586865 1111 4 0.6299737132297096 1111 5 0.36965186058811383 1111 6 0.6270679150648064 1111 7 0.7769984122866198 1111 8 0.9223626425326081 1111 9 0.3170307833977901 1111 10 0.40828973311303685 1111 11 0.3803529285203595 1111 12 0.3266190782829099 1111 13 0.17547303435047468 1111 14 0.2942870779896367 1111 15 -0.7188377796164929 1111 16 -0.2582994751393457 1111 17 -0.10087910970906933 1111 18 0.16725108268290256 1111 19 0.5936530119229033 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18 0.9413372263246984 2207 19 1.2464368386697302 2207 20 1.2634853090175255 2207 21 1.2164782860753078 2207 22 1.2001991292614136 2207 23 1.1035122280129883 2207 24 1.8424574731369816 2207 25 1.3787960805284385 2208 1 0.6621336909817543 2208 2 0.5318936949632054 2208 3 0.30604366538904415 2208 4 0.7044076180965604 2208 5 0.8447880165771338 2208 6 1.3172347788881922 2208 7 1.301393057677057 2208 8 1.1492522166004413 2208 9 0.844022594150907 2208 10 0.9205868617942556 2208 11 0.9143819820060557 2208 12 0.7567607806857737 2208 13 0.8853948542224046 2208 14 1.0094695203857498 2208 15 1.4330588287477681 2208 16 1.0842125715536497 2208 17 0.9360152226367617 2208 18 0.8981660420902244 2208 19 -0.02008971991792663 2208 20 0.7758763084500586 2208 21 0.8309241020289733 2208 22 1.0061165542095267 2208 23 0.9427496790860559 2208 24 1.8256536810414588 2208 25 1.5150144778204933 2209 1 0.42407842160414333 2209 2 0.31821876617101097 2209 3 -0.4960077254357529 2209 4 0.24809739447374568 2209 5 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-1.153746681409018 2215 1 0.452047770263146 2215 2 -0.08164439748525609 2215 3 0.34780727182752 2215 4 0.5735626380424392 2215 5 0.7670799796423822 2215 6 1.1655749468241439 2215 7 0.8679941679512773 2215 8 -0.4039349399259109 2215 9 0.21071018307048206 2215 10 -0.20769305005331518 2215 11 -0.2389627888255319 2215 12 -0.17636193530162103 2215 13 0.10472701590957456 2215 14 0.08258721503244004 2215 15 0.045065412283349815 2215 16 0.056835106214848 2215 17 -0.03243820442826717 2215 18 -1.025957315512926 2215 19 0.15618444528418252 2215 20 -3.343744580327243 2215 21 -1.8886130977367017 2215 22 -0.8532552202764876 2215 23 -0.838103669869227 2215 24 -0.47668961128425735 2215 25 -0.10185583210780891 2216 1 -0.5287443783523464 2216 2 -0.8565555186108895 2216 3 -0.21343402805731415 2216 4 -0.4357408520181494 2216 5 -0.666308433556754 2216 6 -2.033180748934708 2216 7 -9.914958613581948 2216 8 -11.217299318274517 2216 9 -5.9438489276400475 2216 10 -3.3716007161691834 2216 11 -2.474599462001642 2216 12 -2.0997157984031176 2216 13 -1.9257146244689807 2216 14 -1.8876145775668292 2216 15 -2.908820221886195 2216 16 -1.9307744276091938 2216 17 -2.3994729047437247 2216 18 -1.93754662000444 2216 19 -1.2543833780596714 2216 20 -2.0897562973168817 2216 21 -4.430674086169926 2216 22 -3.4137203947795136 2216 23 -3.211745450451069 2216 24 -3.5494551212737413 2216 25 -3.074098083372146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33791.0 3 3.0 4 308.0 5 1830.0 6 2356.0 7 1902.0 8 1556.0 9 1332.0 10 1244.0 11 1184.0 12 1198.0 13 1133.0 14 1143.0 15 1184.0 16 1348.0 17 1472.0 18 1723.0 19 1978.0 20 2661.0 21 3533.0 22 4288.0 23 5351.0 24 6179.0 25 7186.0 26 8320.0 27 9155.0 28 10174.0 29 12034.0 30 14495.0 31 17698.0 32 23119.0 33 30592.0 34 41416.0 35 58836.0 36 85410.0 37 140231.0 38 209385.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.51827614027034 18.39749346585532 11.991950953978412 26.09227943989594 2 14.25462094380841 20.320083252758216 42.095748718285705 23.32954708514767 3 19.13451518983777 24.066904218125345 29.02691966746372 27.771660924573165 4 11.415978537333194 16.44072975211263 39.56870974744916 32.57458196310501 5 12.287029992606325 37.913483278505836 37.225817858405755 12.573668870482088 6 31.949530532152853 37.73168673569161 18.234774106269843 12.084008625885694 7 27.74959471456758 31.70708776058141 22.51654383865409 18.026773686196925 8 24.11780427106962 38.31862055813192 20.739708108209744 16.823867062588725 9 26.052988629986352 15.437047037755839 20.151001924691908 38.3589624075659 10 13.535701032737055 29.82612668008997 35.784894897670746 20.853277389502225 11 34.51936418817201 22.613696633968893 24.730122844331106 18.13681633352798 12 22.894832359397665 24.98248790895588 32.51422731832629 19.608452413320173 13 28.954058057586142 22.370948170331538 25.71112105196896 22.96387272011336 14 19.69591038035311 22.780737776277686 28.18494499224507 29.338406851124134 15 22.47633741962357 32.25999574093643 23.08385667581428 22.17981016362572 16 21.15621584553072 28.709175363778584 28.85155262199173 21.28305616869897 17 20.925432586895752 29.33715325074704 28.515844219160396 21.221569943196812 18 20.803220812060367 28.222017454101284 30.494050712253767 20.480711021584582 19 23.205292371655435 27.430938782508065 27.569704145816143 21.79406470002036 20 22.86113034591848 28.169713951255055 28.624429261968253 20.344726440858214 21 22.095996292689982 27.51914260419778 27.407173269866185 22.97768783324605 22 22.160075647382477 29.129733653709643 27.79130537273212 20.91888532617576 23 21.33222254516037 28.73039322704948 28.122451002321135 21.814933225469016 24 22.654667895295137 27.54194837617461 28.889600231439317 20.913783497090936 25 21.906093590325685 28.0489715886033 28.594484334422216 21.4504504866488 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 211.0 1 211.0 2 286.0 3 361.0 4 361.0 5 361.0 6 578.5 7 796.0 8 796.0 9 796.0 10 1109.0 11 1422.0 12 1422.0 13 1422.0 14 2537.0 15 3652.0 16 3652.0 17 3652.0 18 6286.0 19 8920.0 20 8920.0 21 8920.0 22 14099.0 23 19278.0 24 19278.0 25 19278.0 26 28057.5 27 36837.0 28 36837.0 29 36837.0 30 48148.0 31 59459.0 32 59459.0 33 59459.0 34 68454.5 35 77450.0 36 77450.0 37 77450.0 38 83459.5 39 89469.0 40 89469.0 41 89469.0 42 93143.0 43 96817.0 44 96817.0 45 96817.0 46 98292.0 47 99767.0 48 99767.0 49 99767.0 50 94453.0 51 89139.0 52 89139.0 53 89139.0 54 77496.0 55 65853.0 56 65853.0 57 65853.0 58 55791.0 59 45729.0 60 45729.0 61 45729.0 62 36604.0 63 27479.0 64 27479.0 65 27479.0 66 20865.5 67 14252.0 68 14252.0 69 14252.0 70 10316.0 71 6380.0 72 6380.0 73 6380.0 74 4326.0 75 2272.0 76 2272.0 77 2272.0 78 1556.5 79 841.0 80 841.0 81 841.0 82 558.0 83 275.0 84 275.0 85 275.0 86 174.0 87 73.0 88 73.0 89 73.0 90 42.0 91 11.0 92 11.0 93 11.0 94 7.0 95 3.0 96 3.0 97 3.0 98 2.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24305388163075092 2 0.14288622132232026 3 0.14529667304097232 4 0.06990309984091019 5 0.021961893436607798 6 0.02115840953039044 7 0.2960838194410966 8 0.28885246428514033 9 0.018078387889890565 10 0.03816548554532453 11 0.014998366249390691 12 0.01620359210871673 13 0.014730538280651571 14 0.01687316203056453 15 0.014060968358803771 16 0.018747957811738363 17 0.018078387889890565 18 0.013927054374434212 19 0.021961893436607798 20 0.017007076014934088 21 0.015400108202499369 22 0.01687316203056453 23 0.01754273195241233 24 0.015534022186868932 25 0.014864452265021132 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 746748.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.16999653891718 #Duplication Level Percentage of deduplicated Percentage of total 1 93.50192361373924 80.57060434178007 2 4.994982789921205 8.608352994389222 3 0.7815556051839013 2.020399313810043 4 0.2531314890707604 0.8724935814847349 5 0.11824189451621984 0.5094451820608836 6 0.07279172632194465 0.37634776831342664 7 0.04430529809326093 0.26724511683463914 8 0.03604434281612838 0.24847527165706615 9 0.02704031479620575 0.20970574491602523 >10 0.14432419204713817 2.3085601531742714 >50 0.013685211529227389 0.8223811749727866 >100 0.010574019410795846 1.94251956930528 >500 7.775014188863308E-4 0.49323408265701485 >1k 6.220011351090646E-4 0.7502357046445602 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1796 0.24050951592772932 No Hit CTGTAGGACGTGGAATATGGCAAGA 1359 0.18198910475823168 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1324 0.1773021153052971 No Hit GTCCTACAGTGTGCATTTCTCATTT 1120 0.1499836624939069 No Hit CTTTAGGACGTGAAATATGGCGAGG 939 0.1257452313230166 No Hit GTATCAACGCAGAGTACTTTTTTTT 908 0.12159389780756025 No Hit CTGTAGGACCTGGAATATGGCGAGA 762 0.10204245608960454 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 1.3391398436955973E-4 0.0 0.0 0.0 5 0.0 1.3391398436955973E-4 0.0 0.0 0.0 6 0.0 1.3391398436955973E-4 0.0 0.0 0.0 7 0.0 1.3391398436955973E-4 0.0 0.0 0.0 8 0.0 1.3391398436955973E-4 0.0 1.3391398436955973E-4 0.0 9 0.0 1.3391398436955973E-4 0.0 1.3391398436955973E-4 0.0 10 0.0 1.3391398436955973E-4 0.0 1.3391398436955973E-4 0.0 11 0.0 1.3391398436955973E-4 0.0 1.3391398436955973E-4 0.0 12 0.0 1.3391398436955973E-4 0.0 1.3391398436955973E-4 0.0 13 0.0 1.3391398436955973E-4 0.0 1.3391398436955973E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGTCCG 40 2.793149E-4 16.597977 9 TAGGAGT 75 5.6759745E-8 15.233463 4 CTATACC 100 6.366463E-10 14.281373 4 TCTAGCA 95 4.660251E-9 14.046881 2 TAGGACC 415 0.0 13.994598 4 GTCTAGC 50 0.0014484287 13.359827 1 GCCTACG 50 0.0014484287 13.359827 1 GAACTGT 50 0.001478128 13.323904 6 TCTATAC 95 7.0513124E-8 13.043532 3 GTATTAG 90 5.106558E-7 12.723644 1 GTATTAA 70 1.0447137E-4 12.269229 1 CTAGGAC 110 3.62852E-8 12.131397 3 CTAGCAC 95 9.962841E-7 12.040183 3 GTAGGAC 770 0.0 12.007608 3 TGTAGGA 815 0.0 11.929384 2 TTTATAC 80 2.7653918E-5 11.914764 3 CTTACAC 80 2.7653918E-5 11.914764 3 TCAATAC 65 7.7931065E-4 11.731461 3 AGGACGT 545 0.0 11.525265 5 CTAGAAC 100 1.8490482E-6 11.438174 3 >>END_MODULE