FastQCFastQC Report
Thu 26 May 2016
SRR1926756_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926756_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746748
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT16970.2272520314751429No Hit
CTGTAGGACGTGGAATATGGCAAGA15510.20770058975718717No Hit
GTATCAACGCAGAGTACTTTTTTTT14260.1909613417109922No Hit
GTCCTAAAGTGTGTATTTCTCATTT13210.17690037335218842No Hit
GTCCTACAGTGTGCATTTCTCATTT12300.16471420077455848No Hit
CTTTAGGACGTGAAATATGGCGAGG12180.1631072329621238No Hit
CTGTAGGACCTGGAATATGGCGAGA8960.11998692999512553No Hit
GGTATCAACGCAGAGTACTTTTTTT8550.11449645663597359No Hit
TATCAACGCAGAGTACTTTTTTTTT7970.1067294455425391No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG402.7821038E-416.60660419
GTCTAAG456.588403E-414.8269521
CTTAGGC400.00520227414.2801293
TAGGACC2900.013.4567194
GTCTATA905.179354E-712.7088161
GTATAGA1051.897024E-812.7088161
CGAAATC550.003091408412.07752913
GTCCTAT959.965406E-712.039931
TCCTATA959.981632E-712.0383112
GTCCTAC9750.011.9267341
TAAAGGT1209.6970325E-911.897714
TGACGAA658.089648E-411.67936910
TCCAACG658.089648E-411.67936918
GTATAGC1156.7328074E-811.6037011
TCCTACA10800.011.4716932
CATTTCT11150.011.40440614
AGGACCT5950.011.3578145
TGTAGGA8350.011.2994322
GACGTGG4200.011.2985217
AGGACGT7000.011.2858285
Produced by FastQC (version 0.11.5)