FastQCFastQC Report
Thu 26 May 2016
SRR1926755_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926755_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14827
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA6184.168071760976597No Hit
GTACATGGGAAGCAGTGGTATCAAC2511.6928576246037634No Hit
CCCATGTACTCTGCGTTGATACCAC2411.6254130977271193No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA1571.05887907196331No Hit
GAGTACATGGGAAGCAGTGGTATCA1310.8835233020840358No Hit
CATGTACTCTGCGTTGATACCACTG1040.7014230795170973No Hit
ACGCAGAGTACATGGGAAGCAGTGG900.6070007418897957No Hit
ACTCTGCGTTGATACCACTGCTTCC690.46536723544884334No Hit
GGTATCAACGCAGAGTACATGGGAA620.41815606663519256No Hit
TATCAACGCAGAGTACATGGGAAGC610.4114116139475281No Hit
GTATCAACGCAGAGTACATGGGAAG590.39792270857219936No Hit
GCGTTGATACCACTGCTTCCCATGT590.39792270857219936No Hit
GTATCAACGCAGAGTACTTTTTTTT570.38443380319687054No Hit
AAACAAAAAAAAAAAAAAAAAAAAA560.37768935050920616No Hit
GTGGTATCAACGCAGAGTACATGGG560.37768935050920616No Hit
GGTATCAACGCAGAGTACTTTTTTT470.3169892763202266No Hit
GAACAAAAAAAAAAAAAAAAAAAAA470.3169892763202266No Hit
GTACTCTGCGTTGATACCACTGCTT400.2697781075065758No Hit
ATACCACTGCTTCCCATGTACTCTG400.2697781075065758No Hit
TATCAACGCAGAGTACTTTTTTTTT380.25628920213124706No Hit
ACACAAAAAAAAAAAAAAAAAAAAA370.24954474944358265No Hit
GCTTCCCATGTACTCTGCGTTGATA350.23605584406825386No Hit
CATGGGAAGCAGTGGTATCAACGCA330.22256693869292507No Hit
GGGAAGCAGTGGTATCAACGCAGAG330.22256693869292507No Hit
CTTCCCATGTACTCTGCGTTGATAC320.21582248600526066No Hit
GATACCACTGCTTCCCATGTACTCT310.20907803331759628No Hit
CAGTGGTATCAACGCAGAGTACATG290.19558912794226746No Hit
CCACTGCTTCCCATGTACTCTGCGT270.1821002225669387No Hit
GTTGATACCACTGCTTCCCATGTAC270.1821002225669387No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA230.1551224118162811No Hit
TACCACTGCTTCCCATGTACTCTGC230.1551224118162811No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA210.14163350644095232No Hit
ATGGGAAGCAGTGGTATCAACGCAG200.1348890537532879No Hit
CTGCTTCCCATGTACTCTGCGTTGA200.1348890537532879No Hit
GCAGAGTACATGGGAAGCAGTGGTA200.1348890537532879No Hit
GCAGTGGTATCAACGCAGAGTACAT190.12814460106562353No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA190.12814460106562353No Hit
ACGCAGAGTACTTTTTTTTTTTTTT190.12814460106562353No Hit
TGATACCACTGCTTCCCATGTACTC180.12140014837795912No Hit
ATCAACGCAGAGTACATGGGAAGCA170.11465569569029473No Hit
AAGCAGTGGTATCAACGCAGAGTAC160.10791124300263033No Hit
GAGTACTTTTTTTTTTTTTTTTTTT150.10116679031496593No Hit
GTACTTTTTTTTTTTTTTTTTTTTT150.10116679031496593No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)