FastQCFastQC Report
Thu 26 May 2016
SRR1926755_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926755_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14827
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT3882.6168476428137857No Hit
GGTATCAACGCAGAGTACTTTTTTT3242.1852026708032644No Hit
GTACATGGGAAGCAGTGGTATCAAC2871.9356579213596816No Hit
CCCATGTACTCTGCGTTGATACCAC2691.8142577729817224No Hit
TATCAACGCAGAGTACTTTTTTTTT2231.5040129493491603No Hit
GAGTACATGGGAAGCAGTGGTATCA1320.8902677547717003No Hit
CATGTACTCTGCGTTGATACCACTG1140.7688676063937411No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1140.7688676063937411No Hit
ACGCAGAGTACATGGGAAGCAGTGG1090.7351453429554191No Hit
GCGTTGATACCACTGCTTCCCATGT990.6677008160787752No Hit
GAGTACTTTTTTTTTTTTTTTTTTT830.5597895730761449No Hit
GTATCAACGCAGAGTACATGGGAAG750.5058339515748297No Hit
TATCAACGCAGAGTACATGGGAAGC740.4990894988871653No Hit
GGTATCAACGCAGAGTACATGGGAA730.4923450461995009No Hit
GTACTTTTTTTTTTTTTTTTTTTTT730.4923450461995009No Hit
ACTCTGCGTTGATACCACTGCTTCC660.44513387738585014No Hit
ATCAACGCAGAGTACTTTTTTTTTT580.3911782558845349No Hit
GCAGAGTACTTTTTTTTTTTTTTTT530.35745599244621296No Hit
GCTTCCCATGTACTCTGCGTTGATA450.30350037094489785No Hit
GGGAAGCAGTGGTATCAACGCAGAG440.2967559182572334No Hit
GTGGTATCAACGCAGAGTACATGGG420.28326701288190465No Hit
CATGGGAAGCAGTGGTATCAACGCA420.28326701288190465No Hit
GTGGTATCAACGCAGAGTACTTTTT400.2697781075065758No Hit
GATACCACTGCTTCCCATGTACTCT390.26303365481891144No Hit
ATACCACTGCTTCCCATGTACTCTG380.25628920213124706No Hit
GTACTCTGCGTTGATACCACTGCTT370.24954474944358265No Hit
CAGTGGTATCAACGCAGAGTACATG350.23605584406825386No Hit
ACATGGGAAGCAGTGGTATCAACGC350.23605584406825386No Hit
AAGCAGTGGTATCAACGCAGAGTAC340.22931139138058945No Hit
GCAGTGGTATCAACGCAGAGTACAT320.21582248600526066No Hit
GCAGAGTACATGGGAAGCAGTGGTA310.20907803331759628No Hit
CTGCTTCCCATGTACTCTGCGTTGA300.20233358062993187No Hit
ATGGGAAGCAGTGGTATCAACGCAG290.19558912794226746No Hit
TACCACTGCTTCCCATGTACTCTGC280.18884467525460308No Hit
CTTCCCATGTACTCTGCGTTGATAC270.1821002225669387No Hit
CCACTGCTTCCCATGTACTCTGCGT210.14163350644095232No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA210.14163350644095232No Hit
GTTGATACCACTGCTTCCCATGTAC190.12814460106562353No Hit
CCATGTACTCTGCGTTGATACCACT180.12140014837795912No Hit
TGATACCACTGCTTCCCATGTACTC170.11465569569029473No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA160.10791124300263033No Hit
CAACGCAGAGTACTTTTTTTTTTTT160.10791124300263033No Hit
GTACATGGTAAGCAGTGGTATCAAC150.10116679031496593No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG1954.940389E-46.3628122
GTACATG2006.722435E-46.2037411
ACATGGG2000.00471869165.726533
AACGCAG2250.00277230425.51443675
CAACGCA2300.00360012035.39455844
ATCAACG2350.0046443165.279782
TCAACGC2400.0059535465.1697843
ACGCAGA2450.00758563265.0642796
CGCAGAG2450.0082248225.03006127
Produced by FastQC (version 0.11.5)