FastQCFastQC Report
Thu 26 May 2016
SRR1926754_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926754_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11313
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA6595.825156899142579No Hit
GTACATGGGAAGCAGTGGTATCAAC3162.7932467073278526No Hit
CCCATGTACTCTGCGTTGATACCAC3142.7755679306991956No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA1651.458499071864227No Hit
CATGTACTCTGCGTTGATACCACTG1381.2198355873773536No Hit
GAGTACATGGGAAGCAGTGGTATCA1341.184478034120039No Hit
GCGTTGATACCACTGCTTCCCATGT950.8397418898612217No Hit
ACGCAGAGTACATGGGAAGCAGTGG920.8132237249182356No Hit
GGTATCAACGCAGAGTACATGGGAA840.7425086184036065No Hit
TATCAACGCAGAGTACATGGGAAGC770.6806329002033059No Hit
ACTCTGCGTTGATACCACTGCTTCC750.6629541235746487No Hit
AAACAAAAAAAAAAAAAAAAAAAAA730.6452753469459913No Hit
GTATCAACGCAGAGTACATGGGAAG650.5745602404313621No Hit
GTGGTATCAACGCAGAGTACATGGG580.5126845222310615No Hit
GCTTCCCATGTACTCTGCGTTGATA540.47732696897374705No Hit
GTATCAACGCAGAGTACTTTTTTTT490.4331300274021037No Hit
ATACCACTGCTTCCCATGTACTCTG450.3977724741447892No Hit
GATACCACTGCTTCCCATGTACTCT420.37125430920180325No Hit
GTACTCTGCGTTGATACCACTGCTT410.36241492088747457No Hit
GCAGAGTACATGGGAAGCAGTGGTA410.36241492088747457No Hit
CATGGGAAGCAGTGGTATCAACGCA400.35357553257314595No Hit
GAACAAAAAAAAAAAAAAAAAAAAA400.35357553257314595No Hit
CAGTGGTATCAACGCAGAGTACATG390.3447361442588173No Hit
GTTGATACCACTGCTTCCCATGTAC390.3447361442588173No Hit
TACCACTGCTTCCCATGTACTCTGC380.33589675594448865No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA370.32705736763015997No Hit
ACATGGGAAGCAGTGGTATCAACGC340.30053920268717405No Hit
CCACTGCTTCCCATGTACTCTGCGT330.2916998143728454No Hit
AAGCAGTGGTATCAACGCAGAGTAC330.2916998143728454No Hit
GGGAAGCAGTGGTATCAACGCAGAG310.2740210377441881No Hit
GCAGTGGTATCAACGCAGAGTACAT300.26518164942985945No Hit
GGTATCAACGCAGAGTACTTTTTTT300.26518164942985945No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA290.25634226111553077No Hit
ATGGGAAGCAGTGGTATCAACGCAG260.22982409617254485No Hit
ACGCAGAGTACTTTTTTTTTTTTTT260.22982409617254485No Hit
CCATGTACTCTGCGTTGATACCACT250.2209847078582162No Hit
CTGCTTCCCATGTACTCTGCGTTGA240.21214531954388757No Hit
ACACAAAAAAAAAAAAAAAAAAAAA220.19446654291523027No Hit
CTTCCCATGTACTCTGCGTTGATAC210.18562715460090162No Hit
TATCAACGCAGAGTACTTTTTTTTT210.18562715460090162No Hit
ATCAACGCAGAGTACATGGGAAGCA190.16794837797224432No Hit
TTCCCATGTACTCTGCGTTGATACC180.15910898965791567No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA150.13259082471492972No Hit
ACCATGTACTCTGCGTTGATACCAC140.12375143640060107No Hit
ACACAAAAAACAAAAAAAAAAAAAA140.12375143640060107No Hit
TGATACCACTGCTTCCCATGTACTC130.11491204808627242No Hit
GTACATGGAAAGCAGTGGTATCAAC130.11491204808627242No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTG700.001323469110.83691612
CTGCGTT700.001323469110.83691611
GAAGCAG803.242037E-410.6675889
AGCAGTG907.8965735E-510.5358911
AAGCAGT907.8965735E-510.5358910
CTCTGCG750.002353272410.1144549
TCTGCGT750.002353272410.11445410
GGAAGCA855.447239E-410.0847068
GGGAAGC855.447239E-410.0847067
TACATGG1251.7735038E-69.9054222
TGGGAAG909.2187803E-49.5244456
GCAGTGG1002.2957684E-49.48230212
ATGGGAA950.00151269029.0231585
ACATGGG1201.3515254E-48.7307413
GTACATG1501.8096944E-58.2545191
TGGTATC1159.308152E-48.24547916
AGTGGTA1159.308152E-48.24547914
GTGGTAT1159.308152E-48.24547915
CAGTGGT1159.308152E-48.24547913
CATGGGA1100.00571826947.7927274
Produced by FastQC (version 0.11.5)