FastQCFastQC Report
Thu 26 May 2016
SRR1926754_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926754_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11313
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC3823.3766463360735437No Hit
CCCATGTACTCTGCGTTGATACCAC3593.173340404843985No Hit
GTATCAACGCAGAGTACTTTTTTTT3152.7844073190135243No Hit
GGTATCAACGCAGAGTACTTTTTTT2832.5015468929550075No Hit
TATCAACGCAGAGTACTTTTTTTTT2251.988862370723946No Hit
GAGTACATGGGAAGCAGTGGTATCA1671.4761778484928842No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1411.2463537523203394No Hit
CATGTACTCTGCGTTGATACCACTG1351.1933174224343674No Hit
ACGCAGAGTACATGGGAAGCAGTGG1231.0872447626624238No Hit
GGTATCAACGCAGAGTACATGGGAA1120.9900114912048086No Hit
TATCAACGCAGAGTACATGGGAAGC1020.9016176080615221No Hit
GCGTTGATACCACTGCTTCCCATGT940.8309025015468929No Hit
ACTCTGCGTTGATACCACTGCTTCC920.8132237249182356No Hit
GTATCAACGCAGAGTACATGGGAAG790.6983116768319632No Hit
GAGTACTTTTTTTTTTTTTTTTTTT740.6541147352603199No Hit
GTACTTTTTTTTTTTTTTTTTTTTT720.6364359586316627No Hit
GCTTCCCATGTACTCTGCGTTGATA690.6099177936886768No Hit
GCAGAGTACTTTTTTTTTTTTTTTT600.5303632988597189No Hit
GTGGTATCAACGCAGAGTACATGGG570.503845133916733No Hit
CATGGGAAGCAGTGGTATCAACGCA560.4950057456024043No Hit
ACATGGGAAGCAGTGGTATCAACGC520.4596481923450897No Hit
CAGTGGTATCAACGCAGAGTACATG490.4331300274021037No Hit
ATCAACGCAGAGTACTTTTTTTTTT490.4331300274021037No Hit
GTACTCTGCGTTGATACCACTGCTT480.42429063908777515No Hit
GCAGAGTACATGGGAAGCAGTGGTA480.42429063908777515No Hit
GCAGTGGTATCAACGCAGAGTACAT470.41545125077344647No Hit
GGGAAGCAGTGGTATCAACGCAGAG470.41545125077344647No Hit
ATACCACTGCTTCCCATGTACTCTG440.38893308583046055No Hit
CTGCTTCCCATGTACTCTGCGTTGA400.35357553257314595No Hit
AAGCAGTGGTATCAACGCAGAGTAC360.31821797931583135No Hit
GTGGTATCAACGCAGAGTACTTTTT340.30053920268717405No Hit
GATACCACTGCTTCCCATGTACTCT340.30053920268717405No Hit
CCACTGCTTCCCATGTACTCTGCGT320.28286042605851675No Hit
GTTGATACCACTGCTTCCCATGTAC320.28286042605851675No Hit
CTTCCCATGTACTCTGCGTTGATAC300.26518164942985945No Hit
ATGGGAAGCAGTGGTATCAACGCAG290.25634226111553077No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA260.22982409617254485No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA260.22982409617254485No Hit
TACCACTGCTTCCCATGTACTCTGC230.2033059312295589No Hit
CCATGTACTCTGCGTTGATACCACT180.15910898965791567No Hit
CAACGCAGAGTACTTTTTTTTTTTT180.15910898965791567No Hit
ATCAACGCAGAGTACATGGGAAGCA180.15910898965791567No Hit
ACCATGTACTCTGCGTTGATACCAC170.15026960134358702No Hit
CTCTGCGTTGATACCACTGCTTCCC170.15026960134358702No Hit
TTCCCATGTACTCTGCGTTGATACC150.13259082471492972No Hit
AACGCAGAGTACTTTTTTTTTTTTT130.11491204808627242No Hit
TGGGAAGCAGTGGTATCAACGCAGA120.10607265977194379No Hit
TGATACCACTGCTTCCCATGTACTC120.10607265977194379No Hit
GTACATGGTAAGCAGTGGTATCAAC120.10607265977194379No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA1000.00247313278.54999914
TACATGG1303.316861E-48.0384622
GTACATG1407.363356E-47.4642861
TGGTATC1150.0087277187.434782516
GTGGTAT1150.0087277187.434782515
ACATGGG1350.00443957657.03703743
GGTATCA1852.5551592E-46.67567541
AGTACTT1650.0042045286.33333314
GAGTACT1650.0042045286.33333313
CTTTTTT1650.0042045286.33333318
GTACTTT1700.00574617046.147058515
TACTTTT1700.00574617046.147058516
ACTTTTT1750.00777198335.971428417
AACGCAG2300.00355801245.3695657
GTATCAA2300.00355801245.3695652
TCAACGC2300.00355801245.3695655
CAACGCA2300.00355801245.3695656
ATCAACG2350.0045916955.2553194
CAGAGTA2400.0058882565.145833511
AGAGTAC2400.0058882565.145833512
Produced by FastQC (version 0.11.5)