FastQCFastQC Report
Thu 26 May 2016
SRR1926753_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926753_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780832
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT23210.29724703905577643No Hit
GTCCTAAAGTGTGTATTTCTCATTT18480.23667062825294044No Hit
CTGTAGGACGTGGAATATGGCAAGA17740.2271935576410803No Hit
CTTTAGGACGTGAAATATGGCGAGG13600.17417318962337608No Hit
GTACATGGGAAGCAGTGGTATCAAC12340.15803655587885743No Hit
GTCCTACAGTGTGCATTTCTCATTT11750.15048051309372565No Hit
CCCATGTACTCTGCGTTGATACCAC10490.134343879349207No Hit
CTGTAGGACCTGGAATATGGCGAGA8920.11423712142944961No Hit
GTATCAACGCAGAGTACTTTTTTTT8920.11423712142944961No Hit
CTGAAGGACCTGGAATATGGCGAGA8270.10591266751362649No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGAC508.482097E-515.2462963
TAGGACC3900.015.1368714
GCGCCAC456.848538E-414.7515713
GTCCTAA5900.013.5814821
TTATACT751.4422923E-512.6954394
CGCCACC751.5035395E-512.64420214
GGACCTG5800.012.6366546
AGGACCT6450.012.2486375
GTCCTAT1251.3169483E-912.2103991
CTAATAC550.002997022112.1277353
AGTCTGC550.003042117512.1035828
TTAGGAC4750.012.0365493
ATAGGAC1052.6079397E-711.7977293
GTATTGC657.74211E-411.74076751
AGGACGT8800.011.4654885
GTGTAAC1104.6973219E-711.2738061
TAGGCTG855.210314E-511.1982575
GGACGTG8550.011.13277156
GAATAGG600.005693152511.129271
TCCTAAA7150.011.0629842
Produced by FastQC (version 0.11.5)