##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926753_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 780832 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.855894737920575 31.0 30.0 33.0 24.0 34.0 2 28.880117618130406 31.0 30.0 33.0 23.0 34.0 3 28.58121337240277 31.0 28.0 33.0 16.0 34.0 4 32.48126869800418 35.0 33.0 37.0 25.0 37.0 5 32.52968884471948 35.0 33.0 37.0 25.0 37.0 6 32.0705068439818 35.0 32.0 37.0 19.0 37.0 7 31.967598663989182 35.0 32.0 37.0 18.0 37.0 8 31.67443573009303 35.0 32.0 37.0 17.0 37.0 9 33.90024358632843 38.0 34.0 39.0 23.0 39.0 10 34.04731235400188 38.0 34.0 39.0 25.0 39.0 11 34.19573736732101 38.0 35.0 39.0 26.0 39.0 12 34.08201251997869 38.0 35.0 39.0 25.0 39.0 13 34.19155593008483 38.0 35.0 39.0 26.0 39.0 14 35.271354145321915 39.0 36.0 41.0 26.0 41.0 15 34.8711617864022 39.0 35.0 41.0 22.0 41.0 16 34.95974294086308 39.0 35.0 41.0 24.0 41.0 17 35.18423553337978 39.0 36.0 41.0 25.0 41.0 18 35.2328798000082 40.0 36.0 41.0 25.0 41.0 19 34.60096025777632 39.0 34.0 41.0 18.0 41.0 20 34.89252105446498 39.0 35.0 41.0 22.0 41.0 21 34.87799424203926 39.0 35.0 41.0 22.0 41.0 22 34.927519620097534 39.0 36.0 41.0 22.0 41.0 23 34.93774973361747 40.0 36.0 41.0 23.0 41.0 24 33.6628980369657 39.0 33.0 41.0 9.0 41.0 25 34.25071078029589 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.1346894423151639 1101 2 -0.3847192321684574 1101 3 -1.3303911744259125 1101 4 -0.5825208350300883 1101 5 -0.23904309021744297 1101 6 -2.4275617020421976 1101 7 -0.6496464508661894 1101 8 -0.09455813375772237 1101 9 -0.12407893183926433 1101 10 -9.762628930282208 1101 11 -4.22599908419015 1101 12 -1.7132000558657268 1101 13 -0.9931826711139706 1101 14 -0.6724666430875459 1101 15 -2.387372551982615 1101 16 -0.4401048861871928 1101 17 -0.3420824560592308 1101 18 -0.4181419672417803 1101 19 -1.0577193314536117 1101 20 -0.1011412078609979 1101 21 -0.26562011580081446 1101 22 -0.2130031186447212 1101 23 -0.3000318840232623 1101 24 -0.3299464461368231 1101 25 -0.29417586040010235 1102 1 -0.15844866919939093 1102 2 -0.22397719642642144 1102 3 -0.5480545874018254 1102 4 -0.4100995063587618 1102 5 -0.14008475688410726 1102 6 -0.9792686756241729 1102 7 -0.14922759888483483 1102 8 0.39231783749324833 1102 9 0.3936099764746501 1102 10 0.4133071128413306 1102 11 0.14538907626051412 1102 12 0.24577673123605592 1102 13 0.17512905188525707 1102 14 -0.18905682374022348 1102 15 0.4789651034175435 1102 16 0.3486723088400083 1102 17 0.14824145613967943 1102 18 -0.08440799288280942 1102 19 0.3570533958191149 1102 20 0.30753691956712714 1102 21 0.03324594869025077 1102 22 -0.07149792245202491 1102 23 -0.27085186265573924 1102 24 -4.092578779081652 1102 25 -2.1013825426067854 1103 1 0.2979146042054026 1103 2 0.09543263249898004 1103 3 0.4403730749012702 1103 4 0.43856480510832085 1103 5 0.047198980140116475 1103 6 1.0270891533980162 1103 7 0.7921878196067098 1103 8 0.9785447593603074 1103 9 0.7140542380953789 1103 10 0.555593027618599 1103 11 0.4349576391854768 1103 12 0.3088287311392719 1103 13 0.40407138982413215 1103 14 0.5407596400727783 1103 15 0.7137994732000053 1103 16 0.7405347716367245 1103 17 0.44990259776622565 1103 18 0.36092738108578715 1103 19 -1.282849089239079 1103 20 -0.00448367229775215 1103 21 0.2590759145721222 1103 22 0.43064655936373697 1103 23 0.3412389012516286 1103 24 1.8575325282960904 1103 25 0.9041132591692929 1104 1 -0.38942375213693126 1104 2 -0.3145163501055066 1104 3 -1.3798644157986821 1104 4 -0.4964500159174605 1104 5 -0.5031393643926378 1104 6 -1.8099149917653676 1104 7 -0.5841156630391104 1104 8 -0.2784022274247704 1104 9 -0.19881466373156087 1104 10 -0.11815545189767818 1104 11 -0.3972193392148 1104 12 -0.3554130346294784 1104 13 -0.40348260949090786 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25 -1.9229206461414705 1106 1 -0.17950035051555702 1106 2 -0.6953475557060997 1106 3 -0.5106734127410526 1106 4 -0.8489723463651018 1106 5 -0.4126498282527038 1106 6 -0.04605893825826257 1106 7 -2.5464440474237016 1106 8 -0.3844886893132795 1106 9 -0.6458262380245401 1106 10 -0.13464430601688804 1106 11 -0.5319241646547468 1106 12 -0.38235500268912403 1106 13 -0.49994125983493376 1106 14 -0.8273588030371641 1106 15 -0.1555842263458942 1106 16 -0.3093411722213375 1106 17 -0.7239350659108936 1106 18 -0.8229357329848526 1106 19 -0.21887592255352928 1106 20 -0.2949471832318338 1106 21 -0.6887108308861727 1106 22 -0.8332167871305884 1106 23 -0.9920864425175253 1106 24 -3.6804287964027047 1106 25 -1.9565044358269148 1107 1 0.27520097938285915 1107 2 0.023019698843430803 1107 3 0.5005105795289708 1107 4 0.28797726943494695 1107 5 0.43436764412684425 1107 6 0.3068893533729451 1107 7 -1.891303025564305 1107 8 -1.7692120816188108 1107 9 -0.2692204534155991 1107 10 0.29555565621034674 1107 11 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23 0.5941092366038916 1108 24 -0.3793548089245675 1108 25 0.0142345075534962 1109 1 0.4556523199015956 1109 2 0.2937888213395965 1109 3 -1.0013085406557778 1109 4 0.37855103229178155 1109 5 0.6618194200200662 1109 6 0.7011792548237565 1109 7 0.8930375683803291 1109 8 1.4410641362395502 1109 9 0.591902674767347 1109 10 0.7389386884729063 1109 11 0.5341467150181529 1109 12 0.289687775147101 1109 13 0.36115248790869003 1109 14 0.4632379535545539 1109 15 -0.9076662832138425 1109 16 0.2088594190271138 1109 17 0.234677542575767 1109 18 0.3127522792774613 1109 19 -1.2705059817402642 1109 20 0.0034434904736997396 1109 21 0.3123598528041569 1109 22 0.4260372000830941 1109 23 0.5915166247127459 1109 24 0.052541866038993135 1109 25 0.4378743716501319 1110 1 0.42924400535768825 1110 2 0.4592745800456939 1110 3 0.8732613847616051 1110 4 0.6129240802241469 1110 5 0.6003309009044315 1110 6 0.7744451246997954 1110 7 0.9783108231451081 1110 8 1.069910019322716 1110 9 0.8018147660353705 1110 10 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-2.711765064779172 2216 12 -2.4319709728670666 2216 13 -1.9171807467498425 2216 14 -1.9182937013204722 2216 15 -3.4468500452891746 2216 16 -2.349701606401382 2216 17 -2.523160936648253 2216 18 -2.154901505394392 2216 19 -1.5516952350680668 2216 20 -2.534583309601288 2216 21 -5.702262518745929 2216 22 -3.9377040055255783 2216 23 -3.414039999819785 2216 24 -3.7818059675558295 2216 25 -2.9931383771337003 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35094.0 3 2.0 4 327.0 5 1867.0 6 2392.0 7 1934.0 8 1523.0 9 1363.0 10 1268.0 11 1231.0 12 1203.0 13 1178.0 14 1236.0 15 1315.0 16 1294.0 17 1465.0 18 1664.0 19 2137.0 20 2718.0 21 3513.0 22 4443.0 23 5374.0 24 6328.0 25 7356.0 26 8377.0 27 9096.0 28 10220.0 29 12172.0 30 14680.0 31 18146.0 32 23515.0 33 31034.0 34 42673.0 35 60454.0 36 89134.0 37 147333.0 38 225773.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.32788646553904 18.47681957689005 12.171631531588197 26.023662425982714 2 14.080272920233675 20.131458289246712 42.3217457660812 23.466523024438416 3 19.055326549510195 24.179618262455463 29.134100638965343 27.630954549069 4 11.536524381413505 16.46137895415405 39.042557417975694 32.95953924645676 5 12.334292766844847 37.902747515272154 37.15582404322447 12.60713567465853 6 32.01788207565296 37.41901187441557 18.250605249337106 12.31250080059436 7 27.539891942996462 31.770968479520295 22.562358859209894 18.12678071827335 8 24.232412901038007 38.12405990409898 20.856481154408865 16.787046040454147 9 26.15841404071469 15.354021578043323 20.08092726921062 38.40663711203137 10 14.046653923613425 29.576993835163652 35.41872716371407 20.95762507750885 11 34.33539338435428 22.399692593422778 24.985366166063592 18.27954785615934 12 23.290031202127278 24.631235942391346 32.460587460741166 19.618145394740214 13 29.119541967133323 22.430289600758265 25.541992750374646 22.908175681733763 14 19.866815076871447 22.8386910014999 27.790024323732354 29.5044695978963 15 22.59019362474175 32.40079565846117 22.83111982652289 22.177890890274192 16 21.185136985511775 28.272723430036773 29.312555478844065 21.229584105607383 17 21.155326586370897 29.169063029574854 28.48493077339864 21.190679610655607 18 20.922639861259476 27.902318063165875 30.666881847687698 20.508160227886947 19 23.48750938301399 27.2104843812847 27.19754671011675 22.104459525584566 20 23.075228380102367 28.029526742118772 28.447604506632373 20.447640371146488 21 22.19686140698729 27.318977574891257 27.17475394885825 23.309407069263205 22 22.36297566730155 29.154535754861865 27.37707164805775 21.10541692977883 23 21.343163714008675 28.670861998516735 28.11444469065939 21.871529596815204 24 23.06981927170725 27.38283401431993 28.64152780090428 20.90581891306854 25 22.090336430450634 27.794514336655883 28.506031565491607 21.609117667401883 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 248.0 1 248.0 2 339.5 3 431.0 4 431.0 5 431.0 6 603.5 7 776.0 8 776.0 9 776.0 10 1126.5 11 1477.0 12 1477.0 13 1477.0 14 2486.0 15 3495.0 16 3495.0 17 3495.0 18 6089.5 19 8684.0 20 8684.0 21 8684.0 22 14200.0 23 19716.0 24 19716.0 25 19716.0 26 28797.5 27 37879.0 28 37879.0 29 37879.0 30 50547.5 31 63216.0 32 63216.0 33 63216.0 34 71868.0 35 80520.0 36 80520.0 37 80520.0 38 85737.0 39 90954.0 40 90954.0 41 90954.0 42 95369.0 43 99784.0 44 99784.0 45 99784.0 46 102487.5 47 105191.0 48 105191.0 49 105191.0 50 99277.0 51 93363.0 52 93363.0 53 93363.0 54 81151.0 55 68939.0 56 68939.0 57 68939.0 58 58880.5 59 48822.0 60 48822.0 61 48822.0 62 39169.0 63 29516.0 64 29516.0 65 29516.0 66 22837.0 67 16158.0 68 16158.0 69 16158.0 70 11758.5 71 7359.0 72 7359.0 73 7359.0 74 5102.0 75 2845.0 76 2845.0 77 2845.0 78 1940.5 79 1036.0 80 1036.0 81 1036.0 82 673.5 83 311.0 84 311.0 85 311.0 86 195.0 87 79.0 88 79.0 89 79.0 90 53.5 91 28.0 92 28.0 93 28.0 94 15.5 95 3.0 96 3.0 97 3.0 98 2.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22937072251137247 2 0.1430525388303758 3 0.145229703700668 4 0.06979734437113233 5 0.020875169050448752 6 0.020747100528666858 7 0.3014733002745789 8 0.29724703905577643 9 0.01677697635342814 10 0.035346912011802796 11 0.013703331830662677 12 0.014855948526699725 13 0.013062989221753207 14 0.014471742961354042 15 0.012166509569279949 16 0.016520839309864352 17 0.017289250440555716 18 0.012038441047498053 19 0.021259374615794434 20 0.014855948526699725 21 0.012806852178189417 22 0.014471742961354042 23 0.015752428179172984 24 0.013062989221753207 25 0.013575263308880783 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 780832.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.15102583366298 #Duplication Level Percentage of deduplicated Percentage of total 1 92.35226244047318 76.79185359985009 2 5.819739694878496 9.678346514280713 3 0.978391296612692 2.440627199402189 4 0.2976417913908885 0.9899688114048597 5 0.1492041245008839 0.6203238005431033 6 0.08195802181535372 0.4088936153546635 7 0.0557880538715795 0.3247183736079842 8 0.038557037594960174 0.25648457833024385 9 0.03142677268668706 0.23518515487854327 >10 0.16025977595213367 2.4752759638716615 >50 0.016155553530377127 0.9297086062731383 >100 0.016615431135556965 2.9215514228497867 >500 9.230794879257817E-4 0.550461320518863 >1k 0.0010769260692467452 1.3766010388341816 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2321 0.29724703905577643 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1848 0.23667062825294044 No Hit CTGTAGGACGTGGAATATGGCAAGA 1774 0.2271935576410803 No Hit CTTTAGGACGTGAAATATGGCGAGG 1360 0.17417318962337608 No Hit GTACATGGGAAGCAGTGGTATCAAC 1234 0.15803655587885743 No Hit GTCCTACAGTGTGCATTTCTCATTT 1175 0.15048051309372565 No Hit CCCATGTACTCTGCGTTGATACCAC 1049 0.134343879349207 No Hit CTGTAGGACCTGGAATATGGCGAGA 892 0.11423712142944961 No Hit GTATCAACGCAGAGTACTTTTTTTT 892 0.11423712142944961 No Hit CTGAAGGACCTGGAATATGGCGAGA 827 0.10591266751362649 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 1.2806852178189418E-4 13 0.0 0.0 0.0 0.0 1.2806852178189418E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTAGAC 50 8.482097E-5 15.246296 3 TAGGACC 390 0.0 15.136871 4 GCGCCAC 45 6.848538E-4 14.75157 13 GTCCTAA 590 0.0 13.581482 1 TTATACT 75 1.4422923E-5 12.695439 4 CGCCACC 75 1.5035395E-5 12.644202 14 GGACCTG 580 0.0 12.636654 6 AGGACCT 645 0.0 12.248637 5 GTCCTAT 125 1.3169483E-9 12.210399 1 CTAATAC 55 0.0029970221 12.127735 3 AGTCTGC 55 0.0030421175 12.103582 8 TTAGGAC 475 0.0 12.036549 3 ATAGGAC 105 2.6079397E-7 11.797729 3 GTATTGC 65 7.74211E-4 11.7407675 1 AGGACGT 880 0.0 11.465488 5 GTGTAAC 110 4.6973219E-7 11.273806 1 TAGGCTG 85 5.210314E-5 11.198257 5 GGACGTG 855 0.0 11.1327715 6 GAATAGG 60 0.0056931525 11.12927 1 TCCTAAA 715 0.0 11.062984 2 >>END_MODULE