FastQCFastQC Report
Thu 26 May 2016
SRR1926753_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926753_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780832
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT22420.2871296258350068No Hit
CTGTAGGACGTGGAATATGGCAAGA21610.27675607557067333No Hit
CTTTAGGACGTGAAATATGGCGAGG17090.21886910372525717No Hit
GTCCTAAAGTGTGTATTTCTCATTT16770.21477091102823656No Hit
GTATCAACGCAGAGTACTTTTTTTT14300.1831379861481087No Hit
GTACATGGGAAGCAGTGGTATCAAC13520.17314864144912093No Hit
GTCCTACAGTGTGCATTTCTCATTT12920.16546453014220727No Hit
CCCATGTACTCTGCGTTGATACCAC12260.15701200770460227No Hit
CTGTAGGACCTGGAATATGGCGAGA10450.13383160526207943No Hit
CTGAAGGACCTGGAATATGGCGAGA9990.1279404532601123No Hit
GGTATCAACGCAGAGTACTTTTTTT8870.11359677882054015No Hit
TATCAACGCAGAGTACTTTTTTTTT7950.10181447481660587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGAGG400.005318537414.2327587
CCCTAAT500.001458931213.347161
TGTAGGA12250.012.3719462
GCATTAA550.002985855312.1337811
TTGAAGT550.003013313512.1189654
ACTGTTC2900.012.1052488
TAGGACC3700.012.095574
GATATAC2450.012.063031
CTGTAGG12650.011.9830511
TCTACAC1750.011.9705693
AGGACGT12350.011.796545
TCCAACG1052.7559327E-711.74767218
GATTAAG657.7809783E-411.7337671
TAGACTG1302.5120244E-911.7194395
CCTACAC1302.5120244E-911.7194393
GTAGGAC12050.011.6951253
GTCTTAG907.159295E-611.6522821
TAGGACG12800.011.53064
GTCCTAC10800.011.3874581
TATACAC3350.011.3696053
Produced by FastQC (version 0.11.5)