FastQCFastQC Report
Thu 26 May 2016
SRR1926752_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926752_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745978
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15330.20550203893412405No Hit
GTCCTAAAGTGTGTATTTCTCATTT12110.16233722710321216No Hit
CTGTAGGACGTGGAATATGGCAAGA12000.1608626527860071No Hit
GTCCTACAGTGTGCATTTCTCATTT10520.1410229256090662No Hit
CTTTAGGACGTGAAATATGGCGAGG9240.12386424264522547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGG2500.013.28066219
CTACACC957.197923E-813.0230134
GTATTAA1352.5465852E-1112.7147481
GATATAC2100.012.7147481
GGACCTG4150.012.3791656
TAGGACC3000.012.3718624
ACATAAC550.003002717212.1245463
TGTAGGA7800.012.0912372
CTTATAC951.0031035E-612.0333853
GACGTGG2700.011.980117
CCCTTAC802.7527949E-511.9200761
GTCCTAC7300.011.8874181
GGACGTG5850.011.8716946
TCCAACG802.904603E-511.85773418
AGGACGT5950.011.8320625
TCTACAC1302.4920155E-911.7248363
CTGTAGG8000.011.6816751
GTAGGAC7600.011.6573423
CGTTTCC752.1005409E-411.38266214
AGGACCT4700.011.3353745
Produced by FastQC (version 0.11.5)