##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1926752_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	745978
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.422946789315503	31.0	28.0	31.0	22.0	34.0
2	28.376878406601804	31.0	28.0	31.0	20.0	34.0
3	28.068338476469815	31.0	28.0	31.0	16.0	34.0
4	31.996940928552853	35.0	32.0	37.0	20.0	37.0
5	32.02154621181858	35.0	32.0	37.0	19.0	37.0
6	31.573518253889524	35.0	32.0	37.0	17.0	37.0
7	31.462498894069263	35.0	32.0	37.0	17.0	37.0
8	31.169719482343982	35.0	32.0	37.0	16.0	37.0
9	33.39352098855462	37.0	33.0	39.0	18.0	39.0
10	33.55235409087131	38.0	34.0	39.0	19.0	39.0
11	33.70652887886774	38.0	34.0	39.0	22.0	39.0
12	33.58668620254217	38.0	34.0	39.0	19.0	39.0
13	33.6921129041339	38.0	34.0	39.0	22.0	39.0
14	34.722672786596924	39.0	34.0	41.0	22.0	41.0
15	34.31074777004147	39.0	34.0	41.0	17.0	41.0
16	34.398738300593315	39.0	34.0	41.0	17.0	41.0
17	34.623026416328635	39.0	34.0	41.0	21.0	41.0
18	34.67334023255378	39.0	35.0	41.0	21.0	41.0
19	33.99485239511085	39.0	34.0	41.0	11.0	41.0
20	34.31356688803155	39.0	34.0	41.0	18.0	41.0
21	34.31643426481746	39.0	34.0	41.0	18.0	41.0
22	34.34078082731662	39.0	34.0	41.0	17.0	41.0
23	34.330331457496065	39.0	34.0	41.0	11.0	41.0
24	32.97681432964511	39.0	32.0	40.0	2.0	41.0
25	33.586958328529796	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.02289198291537531
1101	2	-0.17206787002047008
1101	3	-1.1285867423567986
1101	4	-0.5030303661954285
1101	5	-0.2930302435798495
1101	6	-2.2917745372435903
1101	7	-0.7409117508779879
1101	8	-0.2517845041103257
1101	9	-0.41961433602718756
1101	10	-9.393222882761016
1101	11	-4.244738565324603
1101	12	-1.8138857361870038
1101	13	-1.0177201710591106
1101	14	-0.921656665138137
1101	15	-2.5269524946392217
1101	16	-0.6855139141589461
1101	17	-0.7011178151452953
1101	18	-0.30116450199430034
1101	19	-1.1277535104821084
1101	20	-0.3084223401130828
1101	21	-0.5413821785534338
1101	22	-0.19848786495224147
1101	23	-0.12795903685392318
1101	24	-0.1967335202611693
1101	25	-0.08000933918509645
1102	1	-8.954025865222093E-4
1102	2	-0.2656958227157702
1102	3	-0.4747109273314223
1102	4	-0.2833859549247819
1102	5	0.17124605743831722
1102	6	-0.7672192108842637
1102	7	0.10802649154320676
1102	8	0.22363388747280766
1102	9	0.2907926829341889
1102	10	0.16349116920386564
1102	11	0.11628754627502502
1102	12	0.05452225445959158
1102	13	0.08992512929599883
1102	14	-0.06955331207323923
1102	15	0.5570374520983137
1102	16	0.49096109122887555
1102	17	0.19753706914982416
1102	18	-0.1535013899133375
1102	19	0.24070666882159486
1102	20	0.12419534530871346
1102	21	-0.05098751966825432
1102	22	0.05757296524539157
1102	23	-0.3399028052654458
1102	24	-4.094471536206122
1102	25	-1.8064221311881425
1103	1	0.01846965170468451
1103	2	-0.2712787714785634
1103	3	0.15268629172522452
1103	4	-0.09096378109513381
1103	5	-0.46579156465577753
1103	6	0.2775654860290153
1103	7	-0.03582432893663423
1103	8	0.06573542913994501
1103	9	-0.1824776899017735
1103	10	-0.2856390807951925
1103	11	-0.5669489392203673
1103	12	-0.5805415522810193
1103	13	-0.5438674320378283
1103	14	-0.6328338774389479
1103	15	-0.1948680996334815
1103	16	-0.0687396771599964
1103	17	-0.36623995891912386
1103	18	-0.4495165245036219
1103	19	-2.1384035346152785
1103	20	-0.6452353398474315
1103	21	-0.7729999989352976
1103	22	-0.5212220595965462
1103	23	-0.6419409351054881
1103	24	1.1076542268232075
1103	25	0.2870610436156227
1104	1	-0.19951730592421768
1104	2	-0.07939205631819135
1104	3	-1.503661622934711
1104	4	-0.4608268251269756
1104	5	-0.44621472206103263
1104	6	-1.756140960976662
1104	7	-0.1842261402540437
1104	8	0.477214487166318
1104	9	0.21027261179992962
1104	10	0.23672227481424102
1104	11	0.2499109272558684
1104	12	0.4588335653549649
1104	13	0.11578425472382747
1104	14	-0.068905222973207
1104	15	0.36353360096771326
1104	16	-0.16035956622233982
1104	17	-0.1727899306864913
1104	18	-0.1777436256207494
1104	19	-1.275744795230196
1104	20	-0.42508720154072677
1104	21	-0.23179252780455073
1104	22	-0.13108466076349146
1104	23	0.06146358875939484
1104	24	1.3040825214612184
1104	25	0.5067508167580783
1105	1	-0.15026161702658314
1105	2	-0.1435842437840371
1105	3	0.2840993231382569
1105	4	-0.17746616759752243
1105	5	-0.3242220230862358
1105	6	-0.49418498572145353
1105	7	0.00529011217780706
1105	8	0.37703524043975634
1105	9	-0.5207283281524155
1105	10	-0.01517621033232075
1105	11	-0.0716664439378718
1105	12	-0.07528014701961894
1105	13	-0.11468550285589885
1105	14	-0.42279946740453767
1105	15	0.23533503056965088
1105	16	-0.2640887725090906
1105	17	-0.2640199566991228
1105	18	-0.35351252849962833
1105	19	-1.0226637188754637
1105	20	-0.2091714037834933
1105	21	-0.2880738140091097
1105	22	-0.3083793467538314
1105	23	-0.4999939931585473
1105	24	-5.291768572599594
1105	25	-2.606745150190573
1106	1	-0.43186799611829585
1106	2	-0.8002366534959613
1106	3	-0.4946105299219745
1106	4	-0.7795302021007835
1106	5	-0.5464184538325405
1106	6	-0.15898248681401128
1106	7	-3.4833824148853303
1106	8	-0.737316283048397
1106	9	-0.7859771800735231
1106	10	-0.4926924912014954
1106	11	-0.9976692666716289
1106	12	-0.5005794356047346
1106	13	-0.8654975761323485
1106	14	-1.0127092688818138
1106	15	-0.16822204381726635
1106	16	-0.24354944409046198
1106	17	-0.9744712358846854
1106	18	-0.8801766160187015
1106	19	-0.25900749059224637
1106	20	-0.2670625207693291
1106	21	-0.7783189046733128
1106	22	-0.8224242382505267
1106	23	-1.0829601561749982
1106	24	-4.198595432988903
1106	25	-2.300791617833017
1107	1	0.07358045316921746
1107	2	-0.06930622600224723
1107	3	0.31511285649881415
1107	4	-0.05279088944361732
1107	5	0.10152789989754751
1107	6	0.14445422735195734
1107	7	-2.4247068071736777
1107	8	-1.5822450340961751
1107	9	-0.5051724860249038
1107	10	-0.19679978374131224
1107	11	-0.5500582021284615
1107	12	-0.07727683711353706
1107	13	-0.3005352961658829
1107	14	-0.14766316661861367
1107	15	-1.690434952450964
1107	16	-0.5805150769488066
1107	17	-0.49752236748569345
1107	18	-0.8038983259935577
1107	19	0.20285336439518176
1107	20	0.20569523279311852
1107	21	-0.05912359872193207
1107	22	-0.18621300606276492
1107	23	-0.4844689575161567
1107	24	-4.489458802645231
1107	25	-2.3200707460811287
1108	1	0.46940076547869225
1108	2	0.11183703208611817
1108	3	0.6469592672949602
1108	4	0.6577769212615294
1108	5	0.695668421741864
1108	6	-3.0943661567255702
1108	7	-1.4239103908381558
1108	8	0.13897440961084584
1108	9	-0.08672572781564725
1108	10	0.5960009749096997
1108	11	0.364171312099387
1108	12	0.5852927796381024
1108	13	0.4570113234543882
1108	14	0.4477719672566707
1108	15	-0.535117044371205
1108	16	0.47108824221904655
1108	17	0.23401913360929427
1108	18	0.528404473866253
1108	19	0.5962015112117101
1108	20	-0.6347420924464515
1108	21	-0.04601443254823323
1108	22	0.2823383035648135
1108	23	0.40814165063794405
1108	24	-0.5982800716297945
1108	25	0.09769247369842304
1109	1	0.4496851858247837
1109	2	0.3699455643515819
1109	3	-0.858784671612959
1109	4	0.39532171763849533
1109	5	0.8206160802580911
1109	6	0.6566856742778349
1109	7	1.028363488778158
1109	8	1.407424998215408
1109	9	0.8431011572538338
1109	10	0.8862601126807661
1109	11	0.5740760109332115
1109	12	0.26719245644883927
1109	13	0.28667989062567045
1109	14	0.5645112394082474
1109	15	-0.9978067930286016
1109	16	0.458069613134775
1109	17	0.20609033872237603
1109	18	0.3580206133239656
1109	19	-1.2462442416261084
1109	20	0.06540035917001319
1109	21	0.4233032828451613
1109	22	0.5386008284421067
1109	23	0.7447199835056395
1109	24	-0.15410144489097632
1109	25	0.027715962693775964
1110	1	0.4283061115411435
1110	2	0.5633051631053689
1110	3	0.739266378305313
1110	4	0.5840722939409417
1110	5	0.6348818360176196
1110	6	0.8369209453844739
1110	7	0.9515734584611515
1110	8	1.2121038755083937
1110	9	0.6895280821039975
1110	10	0.8028890577329477
1110	11	0.5236715514001204
1110	12	0.4256393038998354
1110	13	0.5023567141863126
1110	14	0.39429454968948363
1110	15	0.7961612413958647
1110	16	0.7283743199540069
1110	17	0.23520304252387803
1110	18	0.46892984394274606
1110	19	-1.406657502869244
1110	20	0.17078197616988433
1110	21	0.127000001064701
1110	22	0.3530684646939761
1110	23	0.5433885502240017
1110	24	-0.24580897663999934
1110	25	-0.048003891449312164
1111	1	0.05625223948727154
1111	2	-0.3449439951437867
1111	3	0.36300375204268676
1111	4	0.30629451616316317
1111	5	0.1643431154788999
1111	6	0.2872576457211764
1111	7	0.32026032714802355
1111	8	0.8568850202895355
1111	9	0.13565602823194212
1111	10	0.06719882204271244
1111	11	-0.13626110853253692
1111	12	-0.0794112011506698
1111	13	-0.20050523867563896
1111	14	-0.1343249789300458
1111	15	-0.8258926306580108
1111	16	-0.5047906632109758
1111	17	-0.32371951639868257
1111	18	-0.093359718346818
1111	19	0.5756657294539878
1111	20	-0.0809015255136174
1111	21	0.19013131419601592
1111	22	0.3825297441552564
1111	23	-0.016375759540309787
1111	24	0.44021795938693487
1111	25	0.039538196092777866
1112	1	0.4214295673857471
1112	2	0.6567685813371114
1112	3	-1.0839829643206116
1112	4	0.3612884317299532
1112	5	0.6337514646260871
1112	6	1.5584226799443393
1112	7	1.5458282784952537
1112	8	1.9529056024095262
1112	9	0.7637113639536608
1112	10	1.2985299655474023
1112	11	1.1160826037943252
1112	12	1.2301850421018514
1112	13	0.8554906936892905
1112	14	0.9647335649473661
1112	15	1.3674349160814998
1112	16	1.3105992652792011
1112	17	0.510542792168593
1112	18	0.9340493059029811
1112	19	1.7623152916300882
1112	20	1.2899645346953221
1112	21	1.407883818174298
1112	22	1.3039299296666513
1112	23	1.2349191326560103
1112	24	2.551777247862823
1112	25	1.726650827603045
1113	1	-0.19991394210263636
1113	2	0.24765145084148799
1113	3	-4.650204602691094
1113	4	-0.95029682093665
1113	5	0.0698530167515159
1113	6	0.7041925313001265
1113	7	1.2651698745999447
1113	8	1.508420145553476
1113	9	1.1026165943450508
1113	10	1.0373009735007912
1113	11	0.6224303255826271
1113	12	0.47052602421028666
1113	13	0.0823230441526448
1113	14	-0.13630808684536078
1113	15	0.40871616920502873
1113	16	0.8832964223337356
1113	17	0.7077676718681687
1113	18	0.5721284971413994
1113	19	1.2141608501524956
1113	20	0.680746023422067
1113	21	0.7221463801093861
1113	22	0.8533908980672607
1113	23	0.566078728846378
1113	24	1.91595270800304
1113	25	1.3182005256025633
1114	1	0.6889706849725847
1114	2	0.6350372865027367
1114	3	0.6458859471793623
1114	4	0.4017020500847863
1114	5	0.9450357520639372
1114	6	1.5638069416983136
1114	7	1.4566699686814921
1114	8	1.5200782966339652
1114	9	0.7817095034912214
1114	10	0.7350307134014997
1114	11	0.3864065012401099
1114	12	0.09594541079859908
1114	13	0.31524362485290425
1114	14	0.4223807058712836
1114	15	-2.182718791497834
1114	16	0.010797182223150514
1114	17	0.1285256796552261
1114	18	0.5266972418040794
1114	19	0.8766063914108742
1114	20	0.7805043062167272
1114	21	0.6846042243392958
1114	22	0.5771790553716087
1114	23	0.13979813425100218
1114	24	1.2900513344124533
1114	25	0.8851793500174452
1115	1	-3.8904003595625873
1115	2	-1.3897585793419331
1115	3	-0.32061323979348444
1115	4	0.20916742331689164
1115	5	0.6812559654396964
1115	6	1.2088825707707578
1115	7	1.0668212560719645
1115	8	1.4095636328996584
1115	9	0.843732800521046
1115	10	-0.7703000248739471
1115	11	0.04151263345353584
1115	12	-0.3192957775078469
1115	13	0.1910559208649758
1115	14	0.032327274194805966
1115	15	0.4160559648864748
1115	16	0.5083573696288326
1115	17	0.3153106251999489
1115	18	0.250553154675643
1115	19	-2.160583751658507
1115	20	-0.5882985551709226
1115	21	-0.16308405871113507
1115	22	0.006708172272041679
1115	23	-0.07310457739926335
1115	24	-1.4592533518994415
1115	25	-0.7018540367378137
1116	1	0.4626127634948567
1116	2	0.029790747238013893
1116	3	0.551782293762404
1116	4	0.2354495114946289
1116	5	0.6846158710457715
1116	6	1.1948242076995008
1116	7	1.1897995569813737
1116	8	-1.4430753679061468
1116	9	0.02998092990978307
1116	10	0.23553484331991115
1116	11	0.5922565611616051
1116	12	0.5422430160917813
1116	13	0.5445079181022265
1116	14	0.564696043620387
1116	15	-0.3477074171786825
1116	16	0.28807850465818774
1116	17	0.5928445839889491
1116	18	0.35637219462039127
1116	19	1.077257275163177
1116	20	0.7105387879855201
1116	21	0.6685393440746381
1116	22	0.49773643289135805
1116	23	0.7921754387167681
1116	24	1.8596786729652948
1116	25	1.1427269463403462
1201	1	0.561043744278777
1201	2	0.2437312185314262
1201	3	0.5717255857645185
1201	4	0.28550974537839124
1201	5	0.32278652392231066
1201	6	0.8374389459024734
1201	7	0.6025706094583079
1201	8	0.9381463515508663
1201	9	0.35337812095403365
1201	10	0.4683902232341097
1201	11	0.5135705412991101
1201	12	0.6011767294372632
1201	13	0.5123929742225783
1201	14	0.5524142078091359
1201	15	1.0832954035300233
1201	16	0.7130285546082362
1201	17	0.6210810534018876
1201	18	0.4691564691693699
1201	19	0.07158647537472973
1201	20	0.764896195284102
1201	21	0.8437832178479212
1201	22	0.8562088428343557
1201	23	0.8259578327932786
1201	24	-0.3713917272227505
1201	25	0.1982082297628054
1202	1	0.1000807941853914
1202	2	-0.09738186093569112
1202	3	-0.5020957792059129
1202	4	-0.46837767155250276
1202	5	-0.08547912347377107
1202	6	0.7550896182115316
1202	7	0.545370046046564
1202	8	0.761554310051995
1202	9	0.5195093499734611
1202	10	0.6572557055778532
1202	11	0.2279369202617616
1202	12	0.5310015629888056
1202	13	0.08565046207633742
1202	14	0.25478947229620275
1202	15	0.7457734534304308
1202	16	0.6122799243564998
1202	17	0.40425827990209484
1202	18	0.35615689110768045
1202	19	-0.15393955139352755
1202	20	0.18289512182340673
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2211	3	0.17597334949504173
2211	4	0.47597158239194925
2211	5	0.575975863318547
2211	6	-1.926360473069355
2211	7	0.06617596961539007
2211	8	0.37388589935937944
2211	9	0.42871801094909756
2211	10	0.46974231941379685
2211	11	0.3596669736024438
2211	12	0.3526723602432398
2211	13	0.31261861065510743
2211	14	0.517173586361622
2211	15	1.003012181005424
2211	16	0.15199476081582475
2211	17	0.276378342319866
2211	18	0.16523194541726127
2211	19	0.5653283656683499
2211	20	0.13786661566831526
2211	21	-0.15811718060420077
2211	22	-3.2480618864363713
2211	23	-1.3723783138187287
2211	24	0.8403606009088875
2211	25	0.5148413093269255
2212	1	-0.5647734352843301
2212	2	-0.13347260370496983
2212	3	-3.3121352602298764
2212	4	-1.5683887990738974
2212	5	-0.6210871919383756
2212	6	0.11550208797213202
2212	7	0.38950598678456316
2212	8	0.17849964646442373
2212	9	0.11361422035951563
2212	10	0.2548187783923055
2212	11	0.34803900443206714
2212	12	0.13261678775028685
2212	13	0.20773021027642358
2212	14	-0.13131167542814381
2212	15	-1.3675798612703218
2212	16	-0.30911786574665
2212	17	-0.01734688673614926
2212	18	-0.03829495562733598
2212	19	0.7791500844041366
2212	20	-0.5904459381524916
2212	21	-0.6949573575376036
2212	22	0.051030714773489194
2212	23	0.3333950210122296
2212	24	1.3752057306939278
2212	25	0.7291898702721369
2213	1	-0.3496826261250128
2213	2	-0.47537360137984663
2213	3	0.30761738214018663
2213	4	-6.291582517197742
2213	5	-1.4493391998163183
2213	6	-3.334190499920517
2213	7	-1.1162428351615894
2213	8	-2.997130736952027
2213	9	-0.9094517910694222
2213	10	-0.2639768720362099
2213	11	-0.16369842629243436
2213	12	-0.020937715257829836
2213	13	-0.10109300216662831
2213	14	-0.2933260734150167
2213	15	-2.7258725790250544
2213	16	-0.8747036057045676
2213	17	-0.42351816458442926
2213	18	-0.25814445571219835
2213	19	0.49079092683724923
2213	20	-1.4254658620134393
2213	21	-0.12410910488311089
2213	22	-0.23925886150431808
2213	23	-0.04948392974525717
2213	24	-0.6622124656241297
2213	25	-0.26456152736178495
2214	1	0.38257004406594675
2214	2	0.30115748791421737
2214	3	0.21048356691380476
2214	4	0.29440302601080504
2214	5	0.5042246510126134
2214	6	0.37673660476535176
2214	7	0.9147475629396062
2214	8	-2.501652979552812
2214	9	-1.2222147644214587
2214	10	-0.6942732002988805
2214	11	-0.18162556694047538
2214	12	-1.260787393396427
2214	13	-0.5313893304292918
2214	14	-0.12066271526778394
2214	15	-1.4988344231650217
2214	16	-0.3291575201865342
2214	17	-0.20177543793286645
2214	18	-0.4138246801161287
2214	19	0.7024013127113093
2214	20	-1.4519250269718995
2214	21	-0.5872748545144972
2214	22	-0.28019279900206584
2214	23	-0.6077606226643013
2214	24	-1.5776474280436688
2214	25	-0.7772022293433025
2215	1	0.21957060836119524
2215	2	-0.360488327354787
2215	3	0.10249121930792882
2215	4	0.49518340505205316
2215	5	0.6251923485503568
2215	6	0.9312344508090895
2215	7	0.5875629833395699
2215	8	-0.5006159149891758
2215	9	-0.21870237057090236
2215	10	-0.33439115176948775
2215	11	-0.5044616322886171
2215	12	-0.6936087320148658
2215	13	-0.2857839517321281
2215	14	-0.1382157383763598
2215	15	-0.3633037791802707
2215	16	-0.2664629415499249
2215	17	-0.6704460409029878
2215	18	-1.3085299390725922
2215	19	-0.08847568468743106
2215	20	-3.7696968865312
2215	21	-2.236000373046778
2215	22	-1.4248402521036283
2215	23	-1.4005321381411306
2215	24	-1.0604928524349866
2215	25	-0.9763241308806627
2216	1	-0.5851712641708104
2216	2	-0.933889568164183
2216	3	-0.2508215586987035
2216	4	-0.5953457489755856
2216	5	-0.5804957347694533
2216	6	-1.963797734505082
2216	7	-10.233751309270083
2216	8	-11.201446866151553
2216	9	-6.271993095291801
2216	10	-3.5663467788941965
2216	11	-2.383111139002388
2216	12	-1.9523561886052612
2216	13	-1.6595879770809248
2216	14	-1.8826406261334583
2216	15	-2.6171162929635727
2216	16	-2.015709592987342
2216	17	-1.9671950537063765
2216	18	-1.79774005695365
2216	19	-1.3383352510282904
2216	20	-2.246336631545283
2216	21	-4.686539846347742
2216	22	-3.4661031980065005
2216	23	-3.5390043031496425
2216	24	-3.8562894129546095
2216	25	-3.322508288299513
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	38962.0
3	4.0
4	348.0
5	1975.0
6	2545.0
7	2025.0
8	1592.0
9	1411.0
10	1325.0
11	1261.0
12	1304.0
13	1267.0
14	1271.0
15	1294.0
16	1433.0
17	1512.0
18	1861.0
19	2265.0
20	2916.0
21	3708.0
22	4679.0
23	5644.0
24	6585.0
25	7561.0
26	8681.0
27	9441.0
28	10721.0
29	12663.0
30	15297.0
31	18638.0
32	23972.0
33	31691.0
34	43717.0
35	60564.0
36	87880.0
37	141384.0
38	186581.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.8779649724223	18.033506975694547	12.000666270844471	26.087861781038686
2	14.550803926279576	19.86172387457703	41.607261979702855	23.980210219440533
3	19.297360201551907	23.601545732390715	28.825036676916966	28.276057389140412
4	11.75987680486771	16.075998948334234	38.60807207129864	33.55605217549941
5	12.735217847191867	37.47837886398194	36.8859932582281	12.900410030598097
6	32.832088601654576	36.72463496064681	17.916091229669757	12.527185208028854
7	28.094496567828546	31.496706217141107	22.039525394306718	18.369271820723622
8	24.814858604641163	37.445048744048066	20.454765308425678	17.28532734288509
9	26.153815212581954	15.418929246611157	19.717630022927477	38.70962551787941
10	14.125767405933265	29.4426400105137	34.99089438831478	21.440698195238262
11	35.078272901326265	22.15144559510466	24.236711840955042	18.53356966261403
12	23.188526271614805	24.472308515330973	31.998691413608494	20.34047379944573
13	29.163918161585283	21.851419837503016	25.458933311881587	23.52572868903011
14	20.338326307773166	22.10410164001244	27.55990496723193	29.997667084982467
15	23.03148286689566	31.42092547454905	22.775940986718794	22.771650671836497
16	21.825536758918716	28.1727872312183	28.105746836698987	21.895929173163996
17	21.60527511895967	28.731056619306262	27.91064047162867	21.7530277901054
18	21.560259725981847	27.8966780762687	29.536255317665578	21.006806880083875
19	23.901774484643212	26.79895520747016	26.87940637763243	22.4198639302542
20	23.308171884427164	27.684789166722528	27.919286719849833	21.08775222900047
21	22.723799053454353	27.005510343625566	26.931502808800463	23.339187794119617
22	22.729174152512833	28.392630173278345	27.182738666652366	21.69545700755646
23	22.143325454084717	27.937321761608708	27.567001545905896	22.35235123840068
24	23.220496236528867	27.133460435956636	27.944879292730214	21.701164034784288
25	22.550059327090022	27.430164976235645	27.8748835244984	22.144892172175933
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	183.0
1	183.0
2	241.5
3	300.0
4	300.0
5	300.0
6	427.5
7	555.0
8	555.0
9	555.0
10	840.0
11	1125.0
12	1125.0
13	1125.0
14	2002.5
15	2880.0
16	2880.0
17	2880.0
18	5105.0
19	7330.0
20	7330.0
21	7330.0
22	12026.0
23	16722.0
24	16722.0
25	16722.0
26	24771.0
27	32820.0
28	32820.0
29	32820.0
30	43887.0
31	54954.0
32	54954.0
33	54954.0
34	63102.0
35	71250.0
36	71250.0
37	71250.0
38	77954.5
39	84659.0
40	84659.0
41	84659.0
42	89503.5
43	94348.0
44	94348.0
45	94348.0
46	96929.5
47	99511.0
48	99511.0
49	99511.0
50	95597.5
51	91684.0
52	91684.0
53	91684.0
54	82041.5
55	72399.0
56	72399.0
57	72399.0
58	62037.0
59	51675.0
60	51675.0
61	51675.0
62	42269.5
63	32864.0
64	32864.0
65	32864.0
66	25329.0
67	17794.0
68	17794.0
69	17794.0
70	12966.5
71	8139.0
72	8139.0
73	8139.0
74	5644.5
75	3150.0
76	3150.0
77	3150.0
78	2140.5
79	1131.0
80	1131.0
81	1131.0
82	752.5
83	374.0
84	374.0
85	374.0
86	234.5
87	95.0
88	95.0
89	95.0
90	62.5
91	30.0
92	30.0
93	30.0
94	16.5
95	3.0
96	3.0
97	3.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.20590419556608908
2	0.1269474434902906
3	0.12855607001815067
4	0.06622179206357293
5	0.024129397917901065
6	0.02252077139004099
7	0.22748660148154504
8	0.22547581832171995
9	0.019839727176940873
10	0.038070827826021676
11	0.016890578542530746
12	0.0180970484384258
13	0.015818160857290698
14	0.017426787385150768
15	0.015013847593360661
16	0.02131430149414594
17	0.018365152859735813
18	0.01541600422532568
19	0.024665606760521087
20	0.017158682963840755
21	0.014477638750740639
22	0.016890578542530746
23	0.018365152859735813
24	0.016622474121220733
25	0.015147899804015668
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	745978.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.03199479961127
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.25983109485762	80.23329303765415
2	5.145145825686078	8.852943178373325
3	0.8354879745945294	2.1563609125636294
4	0.27196287173677286	0.9359003346778156
5	0.1300769716354408	0.5595390673644713
6	0.08144979398574707	0.4204372951566721
7	0.05310603441782974	0.3198172653803894
8	0.03809958602785237	0.26222267096124297
9	0.01944751383338281	0.15057975680810623
>10	0.13984362767594746	2.214544587350128
>50	0.013398077993978465	0.8096627390175186
>100	0.010748632079692249	1.9433993362625115
>500	7.788863750660231E-4	0.4717444059269439
>1k	6.231091000528184E-4	0.6695554125031282
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	1533	0.20550203893412405	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1211	0.16233722710321216	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1200	0.1608626527860071	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1052	0.1410229256090662	No Hit
CTTTAGGACGTGAAATATGGCGAGG	924	0.12386424264522547	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.681044213100118E-4
2	0.0	0.0	0.0	0.0	2.681044213100118E-4
3	0.0	0.0	0.0	0.0	2.681044213100118E-4
4	0.0	0.0	0.0	0.0	2.681044213100118E-4
5	0.0	0.0	0.0	0.0	2.681044213100118E-4
6	0.0	0.0	0.0	0.0	2.681044213100118E-4
7	0.0	0.0	0.0	0.0	2.681044213100118E-4
8	0.0	0.0	0.0	0.0	2.681044213100118E-4
9	0.0	0.0	0.0	0.0	2.681044213100118E-4
10	0.0	0.0	0.0	0.0	4.0215663196501773E-4
11	0.0	0.0	0.0	1.340522106550059E-4	4.0215663196501773E-4
12	0.0	0.0	0.0	1.340522106550059E-4	4.0215663196501773E-4
13	0.0	0.0	0.0	1.340522106550059E-4	4.0215663196501773E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGAGG	250	0.0	13.280662	19
CTACACC	95	7.197923E-8	13.023013	4
GTATTAA	135	2.5465852E-11	12.714748	1
GATATAC	210	0.0	12.714748	1
GGACCTG	415	0.0	12.379165	6
TAGGACC	300	0.0	12.371862	4
ACATAAC	55	0.0030027172	12.124546	3
TGTAGGA	780	0.0	12.091237	2
CTTATAC	95	1.0031035E-6	12.033385	3
GACGTGG	270	0.0	11.98011	7
CCCTTAC	80	2.7527949E-5	11.920076	1
GTCCTAC	730	0.0	11.887418	1
GGACGTG	585	0.0	11.871694	6
TCCAACG	80	2.904603E-5	11.857734	18
AGGACGT	595	0.0	11.832062	5
TCTACAC	130	2.4920155E-9	11.724836	3
CTGTAGG	800	0.0	11.681675	1
GTAGGAC	760	0.0	11.657342	3
CGTTTCC	75	2.1005409E-4	11.382662	14
AGGACCT	470	0.0	11.335374	5
>>END_MODULE