FastQCFastQC Report
Thu 26 May 2016
SRR1926752_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926752_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745978
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15180.20349125577429897No Hit
CTGTAGGACGTGGAATATGGCAAGA14240.1908903479727284No Hit
GTCCTAAAGTGTGTATTTCTCATTT11300.1514789980401567No Hit
GTATCAACGCAGAGTACTTTTTTTT11170.1497363193016416No Hit
CTTTAGGACGTGAAATATGGCGAGG10500.14075482118775623No Hit
GTCCTACAGTGTGCATTTCTCATTT10490.1406207689771012No Hit
CTGTAGGACCTGGAATATGGCGAGA9270.12426639927719048No Hit
GTACATGGGAAGCAGTGGTATCAAC7960.10670555968138469No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCA250.006070023418.97781613
CTAGGAC1300.014.6464223
ACCGTGT400.005316748714.233368
TAGGACC3000.013.9619844
TAGGACT1101.8007995E-1013.8465954
GTAGGAC7450.012.7787593
GTCCTAA3800.012.5408831
TAGGACA2750.012.4619374
TGTAGGA7800.012.3415162
CTTACTC701.0680631E-412.2402253
GGCGAGG3150.012.05021419
TTAACAC802.800195E-511.9002183
TAAGACA657.8778574E-411.7163514
TCCAACG907.537743E-611.598330518
GTTCAGA1406.4028427E-1011.5734431
ATAACAC752.0306352E-411.424213
ACTGTTC2250.011.3866888
GATATAC2350.011.356171
TGGCGAG7750.011.26500618
CTGTAGG8250.011.2062291
Produced by FastQC (version 0.11.5)