FastQCFastQC Report
Thu 26 May 2016
SRR1926751_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926751_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445697
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT8910.1999115991357357No Hit
GGTATCAACGCAGAGTACTTTTTTT5190.11644682373899756No Hit
TATCAACGCAGAGTACTTTTTTTTT4910.11016452881666246No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAG401.2307433E-519.08981
GTAGGAC250.005901894519.0682623
GCGCCAC350.002191718216.25800913
AGACCCG508.5769265E-515.2236925
TAGCACT456.646907E-414.8074814
GTAGCCC602.4749625E-514.3011973
CTAAAGT400.0052014914.2786424
TAGTATT400.0052014914.2786424
GACTGCC707.1274826E-613.5910517
CATAATA550.002981330312.1343482
CAAGACT550.003016800612.1152114
GGTATCA2600.011.7475691
TCTATTC657.766353E-411.7343153
GTGTAGC1001.8133051E-611.453881
CCAGTAC600.00570994211.1231543
CTGGACT1053.365496E-610.8789644
GCACTGT1355.5122655E-810.5720086
ATAGACT750.00259757110.1537014
AATTCTG750.002607301810.1491285
TAGCCCT1053.9971128E-59.97238354
Produced by FastQC (version 0.11.5)