FastQCFastQC Report
Thu 26 May 2016
SRR1926751_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926751_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445697
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17160.38501493166882433No Hit
GGTATCAACGCAGAGTACTTTTTTT10590.2376053686697465No Hit
TATCAACGCAGAGTACTTTTTTTTT10430.23401548585698356No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6140.13776175293977747No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAC250.005876471819.0825161
AGCACCG350.00212420616.3343165
GGTATCA3100.015.0813431
GGACCAT456.6037336E-414.8202086
GTGCATA400.0051218214.3118861
TAGGACC400.00516804614.2925274
GTAAAAA400.00533260914.22517813
GCCCTAA500.001448509913.357761
AAGGCTA603.980457E-412.7044695
CCCTTAA550.002964684312.1434191
GAGTCTG550.003094979612.0739119
CTGATCA802.8970286E-511.8583069
TCTTGTA657.717797E-411.7430872
AACTGAT658.091116E-411.6771817
GTATAAA855.0737275E-511.2250081
CTAGAAG855.1383166E-511.2110883
TTAGACA600.00573579411.116414
TAGCACC600.00573579411.116414
AAGAGTA600.00592129611.06899457
ATACAGT909.207445E-510.5858626
Produced by FastQC (version 0.11.5)