FastQCFastQC Report
Thu 26 May 2016
SRR1926750_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926750_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784159
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT13090.16693043120081513No Hit
GTCCTAAAGTGTGTATTTCTCATTT11430.14576125505159032No Hit
CTGTAGGACGTGGAATATGGCAAGA9940.12676000658029812No Hit
CTTTAGGACGTGAAATATGGCGAGG8830.11260471409497308No Hit
GTCCTACAGTGTGCATTTCTCATTT8630.11005421094446408No Hit
GTATCAACGCAGAGTACTTTTTTTT7950.10138250023273342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3000.014.5949794
GGACCTG4250.012.9849186
AGGACCT4600.012.8243115
GTAGGAC6950.012.2023913
AGGACGT5150.011.8242315
GTATAAG1052.5824556E-711.806681
TAGGACT1701.8189894E-1211.7581044
CTTCTAG907.1386785E-611.65531351
GGACGTG5350.011.5600526
TGTAGGA7600.011.5356432
GACCTGG4600.011.3756777
GTCCTAC7550.011.2413071
GTAATTC600.005740255611.1169723
TAGCCTT600.005803542711.1006135
GACTGAT600.005806307311.0999037
CCTACCC951.3128325E-511.0333863
CTAGCAC700.001452763310.8900953
GGCGAGA3750.010.8762219
GACGTGA3150.010.8733747
TGGCGAG6200.010.86194118
Produced by FastQC (version 0.11.5)