FastQCFastQC Report
Thu 26 May 2016
SRR1926750_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926750_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784159
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12960.16527260415298428No Hit
CTGTAGGACGTGGAATATGGCAAGA12310.15698346891382997No Hit
GTCCTACAGTGGACATTTCTAAATT12180.1553256418659991No Hit
GTCCTAAAGTGTGTATTTCTCATTT11760.14996958524993018No Hit
CTTTAGGACGTGAAATATGGCGAGG11220.14308322674355584No Hit
GTCCTACAGTGTGCATTTCTCATTT8410.10724865747890415No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTCG456.823951E-414.75858411
TAGGACC3150.012.9986564
GTCCTAT905.1776806E-712.7093771
CCAATAG604.008018E-412.69636253
GGCGAGG3350.012.46299519
GTATAAG853.7908267E-612.3355721
GGACTGC701.0660028E-412.2429216
GTAGGAC7500.011.9345823
GAATAAG907.1732356E-611.6502631
TGTAGGA7750.011.5621742
CTGTAGG8100.010.9441861
CTAAATT3500.010.84442319
CCTGGAC1855.456968E-1210.8090653
AGGACCT5500.010.7341985
GTCCTAC6950.010.6978211
TGGCGAG7850.010.63720618
TAGGACG7850.010.5533474
AGGACGT7600.010.5246165
GTATTAA1002.306862E-510.4852361
GGGTAAG1002.306862E-510.4852361
Produced by FastQC (version 0.11.5)