FastQCFastQC Report
Thu 26 May 2016
SRR1926749_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926749_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235268
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC7080.30093340360780046No Hit
CCCATGTACTCTGCGTTGATACCAC6390.271605148171447No Hit
GTATCAACGCAGAGTACTTTTTTTT4640.19722189162997092No Hit
GGTATCAACGCAGAGTACTTTTTTT3070.13048948433276095No Hit
GAGTACATGGGAAGCAGTGGTATCA2940.12496387098967987No Hit
TATCAACGCAGAGTACTTTTTTTTT2520.1071118894197256No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACTG350.002136713416.315345
ATATAGA456.5014424E-414.8458391
TAGGACC456.6184584E-414.8109814
CAGGACT400.0051836514.2820174
GGACTGT400.005198359514.2759236
ACTTTAG400.005198359514.2759237
CCCTGGC500.00147262213.3241947
GTATATT603.9142254E-412.7250041
GTCCAGG550.002951688812.1465941
GTAGAAC550.002970679212.1362093
AGAACAG752.025049E-411.4207375
AGGACGT600.00577184311.1034965
ATACAAT600.00577184311.1034966
TGTAGAA700.001437649210.89781952
TAGAACA700.001455713610.88153654
AACAGTG700.001460911110.8768937
ACACTGT800.0044244999.51728256
TCCAGGA1054.2917999E-49.0815162
CTGTAGA950.00173722679.041451
GGACCTG850.0072861318.9574426
Produced by FastQC (version 0.11.5)