##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926749_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 235268 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.048128092218235 31.0 30.0 33.0 25.0 34.0 2 29.08163456143632 31.0 30.0 33.0 25.0 34.0 3 28.76251763945798 31.0 30.0 33.0 22.0 34.0 4 32.65313599809579 35.0 33.0 37.0 26.0 37.0 5 32.57045582059609 35.0 33.0 37.0 25.0 37.0 6 32.11922148358468 35.0 32.0 37.0 19.0 37.0 7 32.15894214257783 35.0 33.0 37.0 19.0 37.0 8 32.006928269037864 35.0 32.0 37.0 18.0 37.0 9 34.15282146318241 38.0 34.0 39.0 25.0 39.0 10 34.31257119540269 39.0 35.0 39.0 26.0 39.0 11 34.454813234269004 39.0 35.0 39.0 27.0 39.0 12 34.36642042266691 39.0 35.0 39.0 27.0 39.0 13 34.46137171226006 39.0 35.0 39.0 27.0 39.0 14 35.57724807453627 40.0 36.0 41.0 27.0 41.0 15 35.13717122600609 40.0 36.0 41.0 24.0 41.0 16 35.21081915092575 39.0 36.0 41.0 25.0 41.0 17 35.442168080656955 40.0 36.0 41.0 27.0 41.0 18 35.51490640461091 40.0 36.0 41.0 27.0 41.0 19 34.92157029430267 39.0 35.0 41.0 21.0 41.0 20 35.169644830576196 40.0 36.0 41.0 25.0 41.0 21 35.18088732849346 40.0 36.0 41.0 25.0 41.0 22 35.199402383664584 40.0 36.0 41.0 25.0 41.0 23 35.211414216978085 40.0 36.0 41.0 25.0 41.0 24 33.943681248618596 39.0 34.0 41.0 10.0 41.0 25 34.56405035958991 39.0 35.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.4320419230077057 1101 2 -0.5773240859763824 1101 3 -1.4770774482800881 1101 4 -0.9564716859399418 1101 5 -0.5244597723795792 1101 6 -2.0570930011458266 1101 7 -0.6162835318629014 1101 8 0.03666361595517742 1101 9 -0.2603585599203839 1101 10 -8.688843467314662 1101 11 -3.3780165333357317 1101 12 -1.1068469324044443 1101 13 -0.6137165570146621 1101 14 -0.6406784195187285 1101 15 -1.6831123891664532 1101 16 -0.15002082419639606 1101 17 -0.12031042325187258 1101 18 0.010207833998435945 1101 19 -0.4330023337435236 1101 20 0.3355883698868709 1101 21 0.2542959070734412 1101 22 0.6036107087915568 1101 23 -0.12607494200117486 1101 24 -0.2682305304963606 1101 25 0.01528948590844692 1102 1 0.10310627236148306 1102 2 -0.09890410203153621 1102 3 -0.4823189839083639 1102 4 -0.1777652276754722 1102 5 -0.11791021389631595 1102 6 -0.7381699557034445 1102 7 -0.26252709799597085 1102 8 0.3914982636075095 1102 9 0.5692128304244832 1102 10 0.33228007867076315 1102 11 0.4104804209070778 1102 12 0.1553715121344439 1102 13 0.02704149810803358 1102 14 0.16074922997521668 1102 15 0.4621395350909907 1102 16 0.47572880984052546 1102 17 0.3205373518500494 1102 18 0.009302764032590005 1102 19 0.09177297279973828 1102 20 0.18688143941198376 1102 21 0.1173234054600556 1102 22 -0.09803887959836288 1102 23 0.0174140633662887 1102 24 -4.188411229682583 1102 25 -2.033702344851342 1103 1 0.5869908258112524 1103 2 0.09849463449763363 1103 3 0.5747601688229622 1103 4 0.5243033961322823 1103 5 -0.13582177093830694 1103 6 0.5558223643758353 1103 7 0.40845023385092816 1103 8 0.48259203296214537 1103 9 0.24031403196046597 1103 10 0.36465961539812497 1103 11 0.22697986349347588 1103 12 -0.14251031618216814 1103 13 0.1610932756380663 1103 14 -0.029316420960000755 1103 15 0.1363127049163353 1103 16 0.23834493767405007 1103 17 -0.08037928384919724 1103 18 -0.15564211395576422 1103 19 -2.024218604061403 1103 20 -0.5050794177662681 1103 21 -0.02510196305227197 1103 22 0.12093795676977237 1103 23 0.168312118612171 1103 24 1.4949530710730201 1103 25 0.8065057562263291 1104 1 0.012345181600391442 1104 2 -0.050064053191487545 1104 3 -1.2314032329926121 1104 4 -0.3452458951561397 1104 5 -0.21162205760816022 1104 6 -2.013607481140969 1104 7 -0.14134272681160098 1104 8 0.47045634256558344 1104 9 0.12191938313103634 1104 10 0.510749757140438 1104 11 0.3326415930682529 1104 12 0.3311956879586191 1104 13 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-0.006325202741635394 2215 2 -0.4393812037026663 2215 3 -0.18974340028800185 2215 4 0.15975656507020375 2215 5 0.0662331717396043 2215 6 0.7478189034197413 2215 7 0.4498086591159023 2215 8 -0.973259145926022 2215 9 -0.3176137205811713 2215 10 -0.6837721388739908 2215 11 -0.6576405128855001 2215 12 -0.975129827322121 2215 13 -0.7605376999189275 2215 14 -0.5597295725931346 2215 15 -0.8774445955379164 2215 16 -0.5513154560097107 2215 17 -0.8755123143488746 2215 18 -1.7799985924329462 2215 19 -0.7884626014656888 2215 20 -4.500493650948179 2215 21 -2.3897353180165766 2215 22 -1.8260228911644631 2215 23 -1.8059902824261158 2215 24 -1.8500177271099751 2215 25 -1.5322353209962571 2216 1 -0.3109615384891704 2216 2 -0.5958908805196543 2216 3 -0.05659546703203233 2216 4 -0.36485229411911035 2216 5 -0.2528750405501512 2216 6 -1.4966157597256569 2216 7 -10.096628058102294 2216 8 -11.332094993497815 2216 9 -5.8989096537837895 2216 10 -3.1081849274778897 2216 11 -2.334484176604434 2216 12 -1.6377817153351373 2216 13 -1.6107837885108225 2216 14 -1.8264722666296578 2216 15 -2.573148382234457 2216 16 -1.760310694295491 2216 17 -2.0150492142995233 2216 18 -1.5439002637012678 2216 19 -0.8008203836005876 2216 20 -2.0633496124276505 2216 21 -4.029679031086616 2216 22 -2.7495286865841493 2216 23 -2.722226794237038 2216 24 -3.2226985588760755 2216 25 -2.4801632417143793 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9822.0 3 0.0 4 64.0 5 509.0 6 687.0 7 521.0 8 455.0 9 405.0 10 379.0 11 367.0 12 372.0 13 381.0 14 422.0 15 378.0 16 411.0 17 406.0 18 488.0 19 591.0 20 746.0 21 1000.0 22 1313.0 23 1433.0 24 1717.0 25 2064.0 26 2329.0 27 2480.0 28 2910.0 29 3507.0 30 4132.0 31 5198.0 32 6797.0 33 9114.0 34 12720.0 35 18258.0 36 27428.0 37 45871.0 38 69593.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.07438900225357 19.871431675179668 13.243645069630524 24.81053425293624 2 14.702427064622405 21.620965888292034 40.43561170371253 23.240995343373033 3 17.762765830651613 25.996918152253496 30.393659225962438 25.84665679113245 4 11.750070176333988 18.675836374307806 38.48852935922628 31.085564090131935 5 12.54745577525625 38.955611578996596 35.631475348505006 12.865457297242145 6 30.07150874090198 37.85159172845385 19.273773892932454 12.803125637711721 7 26.194282109319385 32.54091058282928 23.962795773176982 17.302011534674357 8 24.231147939284664 36.32775369453928 21.928108336210535 17.51299002996552 9 25.528749070039325 16.157721330640875 21.30895950685514 37.00457009246466 10 14.132098576457983 30.2949130057656 35.07874551422692 20.4942429035495 11 32.074428965570014 24.33097678452245 24.80327847945211 18.791315770455423 12 21.49191422789804 26.858622272858675 31.968558164844325 19.680905334398965 13 27.241616786831724 23.593728744388518 26.297017412596922 22.867637056182833 14 20.37427517132314 23.71996531025218 28.46217287057663 27.443586647848043 15 23.30020320191809 30.943230995519354 23.985053181087768 21.771512621474788 16 21.176410570349965 29.90851273679557 27.933799272183112 20.98127742067136 17 20.918783797571677 29.798404924667548 28.214808012787813 21.068003264972962 18 20.74963971891699 29.202110248136954 29.463978268355206 20.584271764590852 19 22.367151196160044 28.778415975443327 28.578170238636798 20.276262589759835 20 22.272103117414243 28.54264178857019 28.79473869921395 20.390516394801615 21 22.25462522105836 28.051455584274247 28.15815875391103 21.53576044075636 22 21.94254085397997 28.689186661451867 27.963932865135106 21.40433961943306 23 21.499749177386853 28.500675945686275 28.034741057536156 21.964833819390712 24 21.69639668746918 28.85965956433758 28.371197306443108 21.07274644175013 25 21.86701582699559 28.06730405431257 28.259880713001262 21.805799405690575 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 269.0 1 269.0 2 306.5 3 344.0 4 344.0 5 344.0 6 406.0 7 468.0 8 468.0 9 468.0 10 620.0 11 772.0 12 772.0 13 772.0 14 1184.5 15 1597.0 16 1597.0 17 1597.0 18 2614.5 19 3632.0 20 3632.0 21 3632.0 22 5421.0 23 7210.0 24 7210.0 25 7210.0 26 10013.5 27 12817.0 28 12817.0 29 12817.0 30 16165.0 31 19513.0 32 19513.0 33 19513.0 34 22069.0 35 24625.0 36 24625.0 37 24625.0 38 26293.5 39 27962.0 40 27962.0 41 27962.0 42 28766.0 43 29570.0 44 29570.0 45 29570.0 46 30553.0 47 31536.0 48 31536.0 49 31536.0 50 29509.0 51 27482.0 52 27482.0 53 27482.0 54 23614.5 55 19747.0 56 19747.0 57 19747.0 58 16381.5 59 13016.0 60 13016.0 61 13016.0 62 10401.0 63 7786.0 64 7786.0 65 7786.0 66 5966.0 67 4146.0 68 4146.0 69 4146.0 70 2944.0 71 1742.0 72 1742.0 73 1742.0 74 1210.5 75 679.0 76 679.0 77 679.0 78 466.5 79 254.0 80 254.0 81 254.0 82 164.0 83 74.0 84 74.0 85 74.0 86 48.0 87 22.0 88 22.0 89 22.0 90 13.5 91 5.0 92 5.0 93 5.0 94 2.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22484995834537633 2 0.1419657581991601 3 0.14621623000153017 4 0.062056888314602925 5 0.02082731183161331 6 0.02210245337232433 7 0.28520665793903127 8 0.2826563748576092 9 0.01827702875019127 10 0.034003774418960506 11 0.01572674566876923 12 0.017001887209480253 13 0.015301698488532226 14 0.017001887209480253 15 0.014451604128058213 16 0.018702075930428278 17 0.018702075930428278 18 0.01487665130829522 19 0.02422768927350936 20 0.017426934389717257 21 0.015301698488532226 22 0.017001887209480253 23 0.017851981569954264 24 0.017001887209480253 25 0.01572674566876923 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 235268.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.95780502135413 #Duplication Level Percentage of deduplicated Percentage of total 1 93.05744986892113 81.85129031355001 2 5.615774610391441 9.879024164493627 3 0.7769542406896763 2.050175688392904 4 0.21952953651926863 0.7723734467836029 5 0.09829261474430294 0.4322801321359238 6 0.05159138182368147 0.27227188219377646 7 0.036303058051400806 0.22351961112348084 8 0.01853235697431715 0.1304052353066496 9 0.020904952594403958 0.16548783698513111 >10 0.08093131201749304 1.338211124593487 >50 0.014532286085437758 0.883681614333412 >100 0.008234962115081387 1.4273545843861752 >500 9.68819072362516E-4 0.5739243657218442 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 708 0.30093340360780046 No Hit CCCATGTACTCTGCGTTGATACCAC 639 0.271605148171447 No Hit GTATCAACGCAGAGTACTTTTTTTT 464 0.19722189162997092 No Hit GGTATCAACGCAGAGTACTTTTTTT 307 0.13048948433276095 No Hit GAGTACATGGGAAGCAGTGGTATCA 294 0.12496387098967987 No Hit TATCAACGCAGAGTACTTTTTTTTT 252 0.1071118894197256 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGACTG 35 0.0021367134 16.31534 5 ATATAGA 45 6.5014424E-4 14.845839 1 TAGGACC 45 6.6184584E-4 14.810981 4 CAGGACT 40 0.00518365 14.282017 4 GGACTGT 40 0.0051983595 14.275923 6 ACTTTAG 40 0.0051983595 14.275923 7 CCCTGGC 50 0.001472622 13.324194 7 GTATATT 60 3.9142254E-4 12.725004 1 GTCCAGG 55 0.0029516888 12.146594 1 GTAGAAC 55 0.0029706792 12.136209 3 AGAACAG 75 2.025049E-4 11.420737 5 AGGACGT 60 0.005771843 11.103496 5 ATACAAT 60 0.005771843 11.103496 6 TGTAGAA 70 0.0014376492 10.8978195 2 TAGAACA 70 0.0014557136 10.8815365 4 AACAGTG 70 0.0014609111 10.876893 7 ACACTGT 80 0.004424499 9.5172825 6 TCCAGGA 105 4.2917999E-4 9.081516 2 CTGTAGA 95 0.0017372267 9.04145 1 GGACCTG 85 0.007286131 8.957442 6 >>END_MODULE