FastQCFastQC Report
Thu 26 May 2016
SRR1926749_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926749_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235268
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT9330.39656901916112686No Hit
GTACATGGGAAGCAGTGGTATCAAC7720.32813642314296887No Hit
CCCATGTACTCTGCGTTGATACCAC7640.32473604570107284No Hit
GGTATCAACGCAGAGTACTTTTTTT7100.3017834979682745No Hit
TATCAACGCAGAGTACTTTTTTTTT5240.22272472244419128No Hit
GAGTACATGGGAAGCAGTGGTATCA3250.13814033357702704No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2840.12071339918730979No Hit
CATGTACTCTGCGTTGATACCACTG2440.10371151197782955No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGCC350.00212426516.3296153
CAGAAGT400.005168279614.2884134
TTACACA400.005168279614.2884134
ATATACT500.001462946813.3358524
TAGGACA603.9776563E-412.700814
TCTACAG600.005734385511.1132093
CTACAGT1501.9917934E-910.795694
GTCCTAC1158.30576E-710.7772781
TACACAA803.6529606E-410.716315
ACAGTGT1355.3669282E-810.5840096
TTCAGAA750.002565974110.1649882
TACAGTG1659.553332E-99.8142635
TTAGGAC800.0043926829.5256083
CAGTGTG1304.2416996E-69.4891587
CTCTGCG2401.8189894E-129.4871419
GTACTCT2451.8189894E-129.3312086
CGTTGAT2357.2759576E-129.28528814
TGCGTTG2357.2759576E-129.28528812
TCCTACA1151.06285835E-49.1153422
CCTACAG1151.067819E-49.1114523
Produced by FastQC (version 0.11.5)