##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926749_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 235268 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.84065831307275 31.0 30.0 33.0 26.0 34.0 2 30.155618273628374 31.0 30.0 34.0 26.0 34.0 3 30.248129792406957 31.0 30.0 34.0 26.0 34.0 4 33.8091835693762 37.0 35.0 37.0 28.0 37.0 5 33.787004607511435 37.0 35.0 37.0 30.0 37.0 6 33.721921383273546 37.0 35.0 37.0 29.0 37.0 7 33.69635904585409 37.0 35.0 37.0 29.0 37.0 8 33.70866416172195 37.0 35.0 37.0 29.0 37.0 9 35.230350068857646 39.0 35.0 39.0 29.0 39.0 10 35.157820018022 39.0 35.0 39.0 29.0 39.0 11 35.176369076967546 39.0 35.0 39.0 29.0 39.0 12 35.08348351667035 39.0 35.0 39.0 29.0 39.0 13 35.084575887923556 39.0 35.0 39.0 29.0 39.0 14 36.19862879779656 40.0 36.0 41.0 29.0 41.0 15 36.172441641022154 40.0 36.0 41.0 29.0 41.0 16 36.12981790978799 40.0 36.0 41.0 28.0 41.0 17 36.11032524610232 40.0 36.0 41.0 28.0 41.0 18 36.09340836832889 40.0 36.0 41.0 28.0 41.0 19 36.14766989135794 40.0 37.0 41.0 28.0 41.0 20 36.10678035261914 40.0 37.0 41.0 28.0 41.0 21 36.06214614822245 40.0 37.0 41.0 28.0 41.0 22 35.97523675127939 40.0 37.0 41.0 27.0 41.0 23 35.90492544672458 40.0 36.0 41.0 27.0 41.0 24 35.83566400870497 40.0 36.0 41.0 27.0 41.0 25 35.737975415271094 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.22063334597265793 1101 2 -0.06197880177679238 1101 3 0.1696906886591414 1101 4 -0.08075000803894739 1101 5 -0.22570638487910344 1101 6 -0.04706402541464172 1101 7 -0.18681761794932328 1101 8 -0.21841864579347003 1101 9 -0.1743547818411315 1101 10 -0.22218114983592585 1101 11 -0.2561956648935464 1101 12 -0.2934761337688556 1101 13 -0.3575952636443276 1101 14 -0.11299413308471884 1101 15 -0.3559299687236006 1101 16 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3 0.20596446047808215 2213 4 0.46790854413477945 2213 5 0.039096410218114386 2213 6 0.2308265851607061 2213 7 0.052583594963799385 2213 8 -0.09876774889108475 2213 9 -0.1281177950922725 2213 10 0.07728340725194727 2213 11 0.3004519227728011 2213 12 -0.21939469531638167 2213 13 -0.00641856748332259 2213 14 -0.13608777168205677 2213 15 -0.07239377306679273 2213 16 -7.71758788530974E-4 2213 17 0.09838304679644949 2213 18 -0.384372561650018 2213 19 0.03611179111682361 2213 20 0.0559179627958315 2213 21 -0.019099429599300777 2213 22 -0.2822979850983103 2213 23 0.05676606837339904 2213 24 -0.19784587531803766 2213 25 0.014705637997124654 2214 1 0.4645497187512433 2214 2 0.43149441838752267 2214 3 0.4606762896667824 2214 4 0.40757049120245625 2214 5 0.47034033312392864 2214 6 0.4687090258549276 2214 7 0.47812670403848045 2214 8 0.592276112744301 2214 9 0.5308523289137455 2214 10 0.42115037050300685 2214 11 0.3973124792681375 2214 12 0.4427188680918519 2214 13 0.5744317595142903 2214 14 0.47858116820631835 2214 15 0.6769901927283186 2214 16 0.6842849163583224 2214 17 0.6907548783826911 2214 18 0.5514333442865578 2214 19 0.5709893168570659 2214 20 0.6994039838142498 2214 21 1.0044164274541245 2214 22 0.6891579867705744 2214 23 0.5145760501254699 2214 24 0.7679492416951632 2214 25 0.8108948837048615 2215 1 0.18185696490389702 2215 2 0.4524518076072255 2215 3 0.30368920436788827 2215 4 -0.007401282055603531 2215 5 0.3782489764358701 2215 6 0.5337336436983549 2215 7 -0.051376041302169995 2215 8 0.1511011584999764 2215 9 -0.14446280525994837 2215 10 0.007196822182436335 2215 11 -0.06277052539765293 2215 12 -0.2123348787181385 2215 13 0.07342642350006656 2215 14 -0.2458794303552736 2215 15 0.10139665781223073 2215 16 0.04432409868503129 2215 17 0.08882927324773959 2215 18 0.19118793918769939 2215 19 -0.003701993430873074 2215 20 -0.06017893062907831 2215 21 0.049720992710739154 2215 22 -0.1494340272525747 2215 23 -0.009809792681224394 2215 24 -0.0032852593746142134 2215 25 -0.017862881871401726 2216 1 -0.32158427394208644 2216 2 -0.26614688792316343 2216 3 -1.6920692686824097 2216 4 -1.6693996014505856 2216 5 -0.20186653181377068 2216 6 0.021856034425681514 2216 7 -0.003578834307653267 2216 8 0.009263430251145621 2216 9 0.09958505196865275 2216 10 0.10116398369391533 2216 11 -0.017500894997375838 2216 12 0.18740902908866985 2216 13 0.17618341601737342 2216 14 -0.10820986931329912 2216 15 0.10829914270679097 2216 16 0.16089939912652085 2216 17 -0.3051125458071198 2216 18 0.04684931061474629 2216 19 -0.024296073966532106 2216 20 0.2785896927420026 2216 21 0.18307566706017298 2216 22 -0.007309529099906342 2216 23 0.03928121341430568 2216 24 0.1602869599576806 2216 25 0.26248470992830875 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5998.0 3 3.0 4 94.0 5 686.0 6 913.0 7 647.0 8 510.0 9 422.0 10 406.0 11 361.0 12 323.0 13 293.0 14 317.0 15 300.0 16 291.0 17 350.0 18 354.0 19 460.0 20 532.0 21 707.0 22 930.0 23 1104.0 24 1278.0 25 1542.0 26 1814.0 27 2052.0 28 2371.0 29 2933.0 30 3626.0 31 4738.0 32 6004.0 33 8014.0 34 10837.0 35 15462.0 36 22950.0 37 39889.0 38 95757.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.87148584122769 20.695980049196095 11.257649652307876 27.174884457268337 2 14.451924795519734 22.449422518788353 38.53506166324802 24.56359102244389 3 17.43000705668302 26.065091524328555 30.328433331349526 26.176468087638906 4 12.0853664756788 19.025111787408402 37.43007973885101 31.45944199806178 5 12.682037287357495 38.82399449106072 35.479524258886144 13.01444396269564 6 30.257040240916577 38.33124322848685 18.302493623047457 13.109222907549118 7 26.4979720939724 33.22279757501552 22.68066219421983 17.598568136792252 8 24.753131814645883 36.79069233614031 20.957546748736434 17.49862910047737 9 25.699055456840924 16.178383479421544 20.997594007974634 37.1249670557629 10 14.412384806338627 30.542047811745505 34.31283367905601 20.73273370285986 11 32.470298187073595 24.310641644173344 24.18227030243778 19.036789866315278 12 21.821589938240383 27.056493260848313 31.601117028737562 19.520799772173746 13 27.72150553631012 23.08503177268187 26.287803115635562 22.905659575372454 14 20.7491031504497 23.740160157777513 27.921349269768946 27.589387422003842 15 23.178315332202683 31.0065756196257 23.88730909662803 21.92779995154359 16 21.309405909031014 29.815911283775453 27.88108829542605 20.99359451176748 17 21.186991800805043 29.59701105552415 28.03708127361678 21.178915870054023 18 20.983907576955446 28.644598029464518 29.60563787372591 20.765856519854122 19 21.936259924851658 28.55509461550232 29.037098117891087 20.471547341754935 20 22.475835862385555 28.17449185177629 28.894527896083584 20.455144389754576 21 21.975761028052084 27.690326091115068 28.671872675256438 21.662040205576407 22 21.97876464287536 28.64010405154972 27.925599741571318 21.455531564003604 23 21.482309536358535 28.10539469881157 28.438206640937143 21.97408912389275 24 21.407167176017037 28.766465335129876 28.840423858849736 20.98594363000336 25 21.804185281377922 27.76500193408798 28.568817400840796 21.8619953836933 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 214.0 1 214.0 2 194.5 3 175.0 4 175.0 5 175.0 6 258.0 7 341.0 8 341.0 9 341.0 10 499.5 11 658.0 12 658.0 13 658.0 14 992.0 15 1326.0 16 1326.0 17 1326.0 18 2352.0 19 3378.0 20 3378.0 21 3378.0 22 5114.0 23 6850.0 24 6850.0 25 6850.0 26 9641.5 27 12433.0 28 12433.0 29 12433.0 30 15840.0 31 19247.0 32 19247.0 33 19247.0 34 21895.5 35 24544.0 36 24544.0 37 24544.0 38 26109.5 39 27675.0 40 27675.0 41 27675.0 42 28523.5 43 29372.0 44 29372.0 45 29372.0 46 30759.5 47 32147.0 48 32147.0 49 32147.0 50 30169.0 51 28191.0 52 28191.0 53 28191.0 54 24175.5 55 20160.0 56 20160.0 57 20160.0 58 16811.5 59 13463.0 60 13463.0 61 13463.0 62 10756.0 63 8049.0 64 8049.0 65 8049.0 66 6071.0 67 4093.0 68 4093.0 69 4093.0 70 2977.0 71 1861.0 72 1861.0 73 1861.0 74 1284.0 75 707.0 76 707.0 77 707.0 78 484.0 79 261.0 80 261.0 81 261.0 82 175.0 83 89.0 84 89.0 85 89.0 86 60.0 87 31.0 88 31.0 89 31.0 90 17.0 91 3.0 92 3.0 93 3.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12326368226873183 2 0.11986330482683578 3 0.01275141540711019 4 0.0 5 0.0059506605233180885 6 0.35278915959671525 7 0.021252359011850318 8 0.008925990784977132 9 0.009351037965214139 10 0.005100566162844076 11 0.005525613343081082 12 4.250471802370063E-4 13 0.001275141540711019 14 0.0 15 0.0021252359011850316 16 4.250471802370063E-4 17 4.250471802370063E-4 18 8.500943604740126E-4 19 0.0 20 8.500943604740126E-4 21 0.01147627386639917 22 0.0 23 0.0 24 4.250471802370063E-4 25 0.006375707703555095 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 235268.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.70961793267331 #Duplication Level Percentage of deduplicated Percentage of total 1 91.78717583044607 78.6704377154664 2 6.6381747880458475 11.379108497074284 3 0.9574715899939946 2.461935724793235 4 0.25348900428190063 0.8690578282854198 5 0.11340112326493654 0.48597834740868506 6 0.0558465455120932 0.28719516472206963 7 0.03567952824040487 0.2140655113452191 8 0.02649211511988092 0.18165032521227134 9 0.01794433237135761 0.13842016844453664 >10 0.0874919839475298 1.4194250784139324 >50 0.016398041474235715 1.0707125006595752 >100 0.0079505655632658 1.2449055844979864 >500 0.002484551738520563 1.577107553676375 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 933 0.39656901916112686 No Hit GTACATGGGAAGCAGTGGTATCAAC 772 0.32813642314296887 No Hit CCCATGTACTCTGCGTTGATACCAC 764 0.32473604570107284 No Hit GGTATCAACGCAGAGTACTTTTTTT 710 0.3017834979682745 No Hit TATCAACGCAGAGTACTTTTTTTTT 524 0.22272472244419128 No Hit GAGTACATGGGAAGCAGTGGTATCA 325 0.13814033357702704 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 284 0.12071339918730979 No Hit CATGTACTCTGCGTTGATACCACTG 244 0.10371151197782955 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAAGCC 35 0.002124265 16.329615 3 CAGAAGT 40 0.0051682796 14.288413 4 TTACACA 40 0.0051682796 14.288413 4 ATATACT 50 0.0014629468 13.335852 4 TAGGACA 60 3.9776563E-4 12.70081 4 TCTACAG 60 0.0057343855 11.113209 3 CTACAGT 150 1.9917934E-9 10.79569 4 GTCCTAC 115 8.30576E-7 10.777278 1 TACACAA 80 3.6529606E-4 10.71631 5 ACAGTGT 135 5.3669282E-8 10.584009 6 TTCAGAA 75 0.0025659741 10.164988 2 TACAGTG 165 9.553332E-9 9.814263 5 TTAGGAC 80 0.004392682 9.525608 3 CAGTGTG 130 4.2416996E-6 9.489158 7 CTCTGCG 240 1.8189894E-12 9.487141 9 GTACTCT 245 1.8189894E-12 9.331208 6 CGTTGAT 235 7.2759576E-12 9.285288 14 TGCGTTG 235 7.2759576E-12 9.285288 12 TCCTACA 115 1.06285835E-4 9.115342 2 CCTACAG 115 1.067819E-4 9.111452 3 >>END_MODULE