FastQCFastQC Report
Thu 26 May 2016
SRR1926748_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926748_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774546
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12270.15841538139761874No Hit
CTGTAGGACGTGGAATATGGCAAGA9830.12691305616451443No Hit
GTATCAACGCAGAGTACTTTTTTTT9390.1212323089913317No Hit
GTCCTACAGTGTGCATTTCTCATTT9010.11632620915994661No Hit
GTCCTAAAGTGTGTATTTCTCATTT8510.10987081464496622No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACACT250.005953176419.041894
TAGGACC3100.014.12785154
GGACCTG4400.012.3299526
TAGTACT853.840316E-612.3212224
GTAGGAC6650.012.1814763
AGGACCT4750.012.0225575
GGCGAGA2950.011.89370719
CTTACAC657.8046595E-411.7294933
TGTAGGA6950.011.6564142
TGCGTTG907.6039396E-611.58936512
GTGTAGG1251.713488E-811.4488881
CTGTAGG6800.011.3647061
GACGTGG2450.011.2660367
TCTATAC855.145398E-511.2120163
GGTCTAC1201.1982775E-711.1308641
GACCTGG4550.011.0867377
GTTCTAG951.294717E-511.04717351
GTTCTAA1303.0555384E-811.0085471
AGGCGTG700.001466766210.8775527
GTCTTAG1158.3211853E-710.7851851
Produced by FastQC (version 0.11.5)