FastQCFastQC Report
Thu 26 May 2016
SRR1926748_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926748_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774546
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16550.21367355844585087No Hit
GTCCTACAGTGGACATTTCTAAATT11740.15157266321173954No Hit
CTGTAGGACGTGGAATATGGCAAGA11440.14769942650275128No Hit
GGTATCAACGCAGAGTACTTTTTTT10140.13091540076380226No Hit
GTCCTACAGTGTGCATTTCTCATTT9610.12407268257792307No Hit
TATCAACGCAGAGTACTTTTTTTTT8330.10754687261957327No Hit
GTCCTAAAGTGTGTATTTCTCATTT7830.10109147810459287No Hit
CTTTAGGACGTGAAATATGGCGAGG7760.10018772287249562No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCCG402.7846085E-416.6047748
TAGGACC2650.015.8125894
GTAGGTC551.9154351E-413.8541473
GTCCTAC5250.013.8015931
CGTGCGC500.001513641313.28210510
TCCAACG500.001513641313.28210518
TCCTACA6700.012.9474772
GTAGGAC6000.012.6996353
CCTACAG6350.012.4496423
GCCGGTT550.003097128812.0746411
GCGCCAC550.003097128812.0746413
TGCGCCA550.003097128812.0746412
ACTGTGC1603.6379788E-1211.8605538
GGACCTG4050.011.7573986
TGTAGGA6750.011.7217622
AAGGCGT657.8510254E-411.7212216
CTGTAGG7150.011.4674631
CTACACT2250.011.428194
AGGACCT4850.011.3889185
CTACAGT8350.011.2913264
Produced by FastQC (version 0.11.5)