FastQCFastQC Report
Thu 26 May 2016
SRR1926747_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926747_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613557
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18050.2941861962295272No Hit
CTGTAGGACGTGGAATATGGCAAGA14910.24300920696854572No Hit
GTCCTACAGTGTGCATTTCTCATTT13090.21334611128224434No Hit
GTCCTAAAGTGTGTATTTCTCATTT12350.2012852921570449No Hit
CTGTAGGACCTGGAATATGGCGAGA9790.1595613773455441No Hit
CTTTAGGACGTGAAATATGGCGAGG8720.14212208482667463No Hit
GTATCAACGCAGAGTACTTTTTTTT6310.1028429306486602No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGAG250.00608462318.9692718
TAGGACC3200.013.3846794
CCAACGT500.00151612213.27848919
GTACTAG655.2484673E-513.2046831
GTGTTAA801.9092222E-613.1129841
GTGTTAG1051.8802893E-812.715621
TCCAACG905.4938755E-712.64618118
TGTAGGA8800.012.3432532
GTATAAT853.766254E-612.3416321
GACGTGG3950.012.2870477
TTAGACT701.06974854E-412.2374214
GTCCTAC8550.011.9348361
GTATTAC802.7489852E-511.9208941
TAGGACT1209.686119E-911.8974934
GTGTAGG1052.5769623E-711.8073621
CTAGGAC1052.6128328E-711.7947953
AGGACCT5650.011.7902775
GTAGGAC8650.011.7843073
ATGCTAC657.756062E-411.7374961
ATAGCAC657.825691E-411.7250043
Produced by FastQC (version 0.11.5)