##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926747_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 613557 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.979721525465443 31.0 30.0 33.0 25.0 34.0 2 29.051783289898086 31.0 30.0 33.0 25.0 34.0 3 28.76892285476329 31.0 30.0 33.0 22.0 34.0 4 32.65570924950738 35.0 33.0 37.0 26.0 37.0 5 32.58657793815407 35.0 33.0 37.0 25.0 37.0 6 32.12886659267191 35.0 32.0 37.0 19.0 37.0 7 32.13193069266588 35.0 32.0 37.0 19.0 37.0 8 31.989950403955948 35.0 32.0 37.0 18.0 37.0 9 34.16673756472504 38.0 34.0 39.0 25.0 39.0 10 34.307487323916114 38.0 35.0 39.0 26.0 39.0 11 34.457020293143096 39.0 35.0 39.0 27.0 39.0 12 34.317127830014165 39.0 35.0 39.0 26.0 39.0 13 34.43446330169813 39.0 35.0 39.0 27.0 39.0 14 35.52810904284362 40.0 36.0 41.0 27.0 41.0 15 35.117240940939475 39.0 35.0 41.0 24.0 41.0 16 35.21520249952327 39.0 36.0 41.0 25.0 41.0 17 35.45315431166134 40.0 36.0 41.0 27.0 41.0 18 35.50610782698266 40.0 36.0 41.0 27.0 41.0 19 34.90472441843219 39.0 35.0 41.0 22.0 41.0 20 35.178979296137115 40.0 36.0 41.0 25.0 41.0 21 35.17820675177693 40.0 36.0 41.0 25.0 41.0 22 35.202709772686156 40.0 36.0 41.0 25.0 41.0 23 35.22046036472569 40.0 36.0 41.0 25.0 41.0 24 33.95317631450705 39.0 34.0 41.0 10.0 41.0 25 34.544006506322965 39.0 35.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.058909747583459904 1101 2 -0.22338903102919971 1101 3 -0.5787164683331092 1101 4 -0.12406797974268358 1101 5 -0.043168154909629664 1101 6 -2.0164263377363483 1101 7 -0.3959254835136399 1101 8 0.07958761585958385 1101 9 0.09275507076917222 1101 10 -9.511056699876569 1101 11 -4.070121080847194 1101 12 -1.630096660405279 1101 13 -0.6583720353290659 1101 14 -0.3519906327759159 1101 15 -2.0739936856387047 1101 16 -0.4593528936050433 1101 17 -0.29340192245825847 1101 18 -0.30369887355153224 1101 19 -1.0193392616154426 1101 20 -0.2129112147403589 1101 21 -0.38866041708398313 1101 22 -0.13765621113297044 1101 23 -0.19019866239516858 1101 24 -0.06606598035745748 1101 25 0.035357996499605804 1102 1 -0.04105580792957397 1102 2 0.04654833926385038 1102 3 -0.3257396128061245 1102 4 -0.07165310021814264 1102 5 0.17940866308644132 1102 6 -0.6935493959129637 1102 7 0.032892329841686774 1102 8 0.5858709569501279 1102 9 0.41701349577957814 1102 10 0.42951236255437664 1102 11 0.30446703592825486 1102 12 0.21014141396271668 1102 13 0.14428080745221905 1102 14 0.13900133141169846 1102 15 0.4444834976664609 1102 16 0.37553332230386616 1102 17 0.2865432379678623 1102 18 -0.015107159240777435 1102 19 0.41200448078424046 1102 20 0.3665845624149213 1102 21 0.4273726891297258 1102 22 0.26965540283197953 1102 23 0.009020102072447855 1102 24 -3.1329672975195777 1102 25 -1.7185429342422864 1103 1 -0.12591006588347042 1103 2 -0.358579519191526 1103 3 0.033601336929308445 1103 4 -0.059141961383708974 1103 5 -0.46437602093939745 1103 6 0.4835374839228237 1103 7 0.009583435367161997 1103 8 0.24942469068743023 1103 9 0.02533336008552567 1103 10 0.044586201503726386 1103 11 -0.20659559351268086 1103 12 -0.31711108881815164 1103 13 -0.2673994511531035 1103 14 -0.1473536732282028 1103 15 -0.1493770840726114 1103 16 -0.13573456896882163 1103 17 -0.5494697660451919 1103 18 -0.46158605844738076 1103 19 -2.1470255034216663 1103 20 -0.5156000626135011 1103 21 -0.5391880689870305 1103 22 -0.4187018265020228 1103 23 -0.46334268285125546 1103 24 0.8049711116397731 1103 25 0.023771116360286726 1104 1 -0.25792732883217795 1104 2 -0.2455024165416262 1104 3 -1.7148899507884998 1104 4 -0.9694967377255672 1104 5 -0.4072658289758877 1104 6 -1.5916485237509264 1104 7 -0.17228448272361874 1104 8 0.27066458222535417 1104 9 0.1387013709556797 1104 10 0.20369144922464955 1104 11 0.11156701580237183 1104 12 0.21810017878748056 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-3.297433838507878 2216 16 -2.2934900540644207 2216 17 -2.8280797489722005 2216 18 -2.184023784452414 2216 19 -1.654992545128536 2216 20 -2.723134447190546 2216 21 -5.123564692776807 2216 22 -3.8499464580541805 2216 23 -3.433850948935394 2216 24 -3.9238663344106435 2216 25 -3.516116053447032 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25524.0 3 3.0 4 196.0 5 1228.0 6 1687.0 7 1326.0 8 1077.0 9 971.0 10 901.0 11 862.0 12 904.0 13 953.0 14 915.0 15 919.0 16 972.0 17 1138.0 18 1278.0 19 1603.0 20 2036.0 21 2647.0 22 3285.0 23 4076.0 24 4754.0 25 5489.0 26 6464.0 27 6811.0 28 7857.0 29 9484.0 30 11348.0 31 14157.0 32 18098.0 33 24211.0 34 33487.0 35 47495.0 36 71141.0 37 116842.0 38 181418.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.25041735681639 18.624568350141953 12.096650364431726 26.02836392860993 2 13.95063783670858 19.896816692127906 43.42381866374188 22.728726807421626 3 19.494615477523414 23.802886326022307 29.137790893155014 27.56470730329926 4 11.202418830083385 16.70137592161634 39.810401034591294 32.285804213708985 5 11.983815963641344 38.88195173479568 36.90768839535475 12.226543906208228 6 31.049918655186016 38.42588819081961 18.654696970190958 11.869496183803415 7 27.440659985517456 31.12422785650186 23.091810497718917 18.343301660261766 8 23.37939996240571 39.47253573500928 20.940511118920554 16.207553183664462 9 25.68503757314946 15.59342755146951 20.406050825631244 38.315484049749784 10 13.14900048422834 30.323128669415517 36.28875660922773 20.239114237128412 11 34.47181480020474 22.59592031010077 25.10065560623201 17.831609283462488 12 22.61466588858932 24.9886711503917 33.364684634167816 19.031978326851167 13 29.07669512177231 22.472904621333015 25.804711148348524 22.64568910854615 14 19.012052670528824 23.239126678555245 28.512210786439198 29.23660986447673 15 22.044167555038293 33.1942909490417 23.18633635542689 21.57520514049312 16 20.46011374880391 29.204002888548015 29.852948128393674 20.4829352342544 17 20.425711570467524 29.828499332482433 29.112242386306026 20.633546710744017 18 20.021972791555147 28.788269006953705 31.49281320193126 19.696944999559893 19 22.78950045318501 27.662869476196377 27.865172568939546 21.682457501679067 20 22.25726841799842 28.710332596548195 29.238144605493936 19.794254379959444 21 21.598373883022596 27.85458836876473 27.788083183642332 22.75895456457034 22 21.41104824499204 29.890150014752077 28.23808056752294 20.46072117273295 23 20.7981169382642 29.349975304375608 28.591501771075638 21.260405986284557 24 22.28218966846569 27.864758647782082 29.62781243428892 20.225239249463307 25 21.369889808958728 28.69808306709265 29.18302145139206 20.74900567255656 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 122.0 1 122.0 2 182.5 3 243.0 4 243.0 5 243.0 6 412.5 7 582.0 8 582.0 9 582.0 10 883.0 11 1184.0 12 1184.0 13 1184.0 14 2147.5 15 3111.0 16 3111.0 17 3111.0 18 5448.5 19 7786.0 20 7786.0 21 7786.0 22 12379.5 23 16973.0 24 16973.0 25 16973.0 26 24495.5 27 32018.0 28 32018.0 29 32018.0 30 42648.5 31 53279.0 32 53279.0 33 53279.0 34 60735.0 35 68191.0 36 68191.0 37 68191.0 38 72970.5 39 77750.0 40 77750.0 41 77750.0 42 79851.5 43 81953.0 44 81953.0 45 81953.0 46 81924.0 47 81895.0 48 81895.0 49 81895.0 50 76434.0 51 70973.0 52 70973.0 53 70973.0 54 60649.5 55 50326.0 56 50326.0 57 50326.0 58 41838.5 59 33351.0 60 33351.0 61 33351.0 62 26093.5 63 18836.0 64 18836.0 65 18836.0 66 13991.0 67 9146.0 68 9146.0 69 9146.0 70 6484.0 71 3822.0 72 3822.0 73 3822.0 74 2606.5 75 1391.0 76 1391.0 77 1391.0 78 924.0 79 457.0 80 457.0 81 457.0 82 286.5 83 116.0 84 116.0 85 116.0 86 76.0 87 36.0 88 36.0 89 36.0 90 23.5 91 11.0 92 11.0 93 11.0 94 6.0 95 1.0 96 1.0 97 1.0 98 2.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2234511219006547 2 0.14032926036211796 3 0.14261103695337188 4 0.05997812754153241 5 0.018417196772264027 6 0.019395101025658577 7 0.2910894994271111 8 0.28717788241353287 9 0.014179611674220978 10 0.03422664886880926 11 0.012875739336361576 12 0.012875739336361576 13 0.012386787209664303 14 0.013527675505291279 15 0.012223803167431876 16 0.016298404223242503 17 0.014668563800918251 18 0.011571866998502177 19 0.01972106911012343 20 0.013201707420826428 21 0.011571866998502177 22 0.013690659547523703 23 0.014342595716453403 24 0.012386787209664303 25 0.012549771251896727 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 613557.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.55939962393492 #Duplication Level Percentage of deduplicated Percentage of total 1 94.48981444582289 81.78981609007441 2 4.317401899722363 7.4742343275040755 3 0.5611172049395284 1.4570990513467807 4 0.18961742291449693 0.6565268114286646 5 0.10378604133233706 0.44918287135359947 6 0.06668223739003587 0.3463184660437326 7 0.04809433747132192 0.29141118837799795 8 0.0305467720955248 0.2115288202430236 9 0.024159569428993756 0.18821140424518243 >10 0.14029972581873731 2.3761361195411292 >50 0.013768701671035293 0.8363882811932053 >100 0.01282553352228765 2.3049098318376755 >500 0.001131664722269705 0.6647962058405817 >1k 7.544431481798034E-4 0.9534405309697932 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1805 0.2941861962295272 No Hit CTGTAGGACGTGGAATATGGCAAGA 1491 0.24300920696854572 No Hit GTCCTACAGTGTGCATTTCTCATTT 1309 0.21334611128224434 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1235 0.2012852921570449 No Hit CTGTAGGACCTGGAATATGGCGAGA 979 0.1595613773455441 No Hit CTTTAGGACGTGAAATATGGCGAGG 872 0.14212208482667463 No Hit GTATCAACGCAGAGTACTTTTTTTT 631 0.1028429306486602 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.6298404223242502E-4 0.0 2 0.0 0.0 0.0 1.6298404223242502E-4 0.0 3 0.0 0.0 0.0 1.6298404223242502E-4 0.0 4 0.0 0.0 0.0 1.6298404223242502E-4 0.0 5 0.0 0.0 0.0 1.6298404223242502E-4 0.0 6 0.0 0.0 0.0 1.6298404223242502E-4 0.0 7 0.0 0.0 0.0 1.6298404223242502E-4 0.0 8 0.0 0.0 0.0 1.6298404223242502E-4 0.0 9 0.0 0.0 0.0 1.6298404223242502E-4 0.0 10 0.0 0.0 0.0 1.6298404223242502E-4 0.0 11 0.0 0.0 0.0 3.2596808446485004E-4 0.0 12 0.0 0.0 0.0 3.2596808446485004E-4 0.0 13 0.0 0.0 0.0 3.2596808446485004E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGCGAG 25 0.006084623 18.96927 18 TAGGACC 320 0.0 13.384679 4 CCAACGT 50 0.001516122 13.278489 19 GTACTAG 65 5.2484673E-5 13.204683 1 GTGTTAA 80 1.9092222E-6 13.112984 1 GTGTTAG 105 1.8802893E-8 12.71562 1 TCCAACG 90 5.4938755E-7 12.646181 18 TGTAGGA 880 0.0 12.343253 2 GTATAAT 85 3.766254E-6 12.341632 1 GACGTGG 395 0.0 12.287047 7 TTAGACT 70 1.06974854E-4 12.237421 4 GTCCTAC 855 0.0 11.934836 1 GTATTAC 80 2.7489852E-5 11.920894 1 TAGGACT 120 9.686119E-9 11.897493 4 GTGTAGG 105 2.5769623E-7 11.807362 1 CTAGGAC 105 2.6128328E-7 11.794795 3 AGGACCT 565 0.0 11.790277 5 GTAGGAC 865 0.0 11.784307 3 ATGCTAC 65 7.756062E-4 11.737496 1 ATAGCAC 65 7.825691E-4 11.725004 3 >>END_MODULE