FastQCFastQC Report
Thu 26 May 2016
SRR1926747_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926747_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613557
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18710.30494314301686726No Hit
CTGTAGGACGTGGAATATGGCAAGA17370.2831032813577223No Hit
GTCCTACAGTGTGCATTTCTCATTT14020.22850362720985987No Hit
GTCCTAAAGTGTGTATTTCTCATTT12570.20487094108615828No Hit
CTTTAGGACGTGAAATATGGCGAGG11760.19166923366533184No Hit
CTGTAGGACCTGGAATATGGCGAGA11000.17928244645566752No Hit
GTATCAACGCAGAGTACTTTTTTTT9730.15858347309214957No Hit
CTGAAGGACCTGGAATATGGCGAGA6940.11311092530930297No Hit
GGTATCAACGCAGAGTACTTTTTTT6880.1121330210559084No Hit
CACACACACACACACACACACACAC6220.10137607426856836No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC307.7708723E-418.9797915
GAATACG400.005311656714.23484113
AATACGG400.005311656714.23484114
GTCCTAC9000.013.9731741
CTTGGGC551.923561E-413.8463653
GTCCTAT903.4911864E-813.761461
CTAGACT707.100447E-613.5979974
TAGGACC3450.013.5191674
AGGACAT2000.013.32603555
TCTACAC801.9486615E-613.0891413
GACGTGA3450.012.9282627
TCCTACA10100.012.9229852
CCTACAG10250.012.7234573
ACCCTAC751.4324349E-512.7028851
GTATAGG751.4324349E-512.7028851
CTACACT1457.2759576E-1212.4726444
GTAGGAC8900.012.1933573
GGACGTG7500.012.18380456
AACTAGA550.002999937212.1254822
GGCTAAC550.002999937212.1254821