FastQCFastQC Report
Thu 26 May 2016
SRR1926746_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926746_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781555
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18090.23146163737676811No Hit
CTGTAGGACGTGGAATATGGCAAGA13900.1778505671385891No Hit
GTCCTAAAGTGTGTATTTCTCATTT13230.1692779139024125No Hit
GTCCTACAGTGTGCATTTCTCATTT12530.16032141052133247No Hit
GTATCAACGCAGAGTACTTTTTTTT10770.13780220202033125No Hit
CTTTAGGACGTGAAATATGGCGAGG9420.12052894549967692No Hit
CTGTAGGACCTGGAATATGGCGAGA8470.10837369091106833No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC508.4278334E-515.2577713
TCCAACG851.7575076E-814.50063818
GCCTAAT500.001447683713.3608681
CTACACT1600.012.4992474
TAGGACC3150.012.3953234
CCAACGA701.1099099E-412.19009719
ATTAACC550.002980178712.1368633
TCTACAC1501.2732926E-1112.0790693
GTCCTAT1209.325959E-911.9293471
TTTAGAC802.75305E-511.9201353
GTAGGAC8200.011.8619883
CTGACCC752.1060248E-411.3796089
ATACACT2850.011.3610534
ACTGTTC2600.011.3393218
TGTAGGA8950.011.2941612
CACTGTT3100.011.0535577
GCCGGTT951.3878429E-510.97821611
GTCCTAC8400.010.9068331
GTCTAAT700.001434250410.9068321
TAAGACA1408.005372E-910.88369754
Produced by FastQC (version 0.11.5)