##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926746_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 781555 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.931263954552144 31.0 30.0 33.0 25.0 34.0 2 28.979458899245735 31.0 30.0 31.0 25.0 34.0 3 28.698108258535868 31.0 29.0 33.0 20.0 34.0 4 32.57067768743083 35.0 33.0 37.0 25.0 37.0 5 32.50515062919436 35.0 33.0 37.0 25.0 37.0 6 32.059174338338316 35.0 32.0 37.0 19.0 37.0 7 32.08178567087409 35.0 32.0 37.0 19.0 37.0 8 31.911687597162068 35.0 32.0 37.0 17.0 37.0 9 34.09132946497687 38.0 34.0 39.0 25.0 39.0 10 34.230535279027066 38.0 35.0 39.0 26.0 39.0 11 34.38049273563601 39.0 35.0 39.0 27.0 39.0 12 34.25309543154353 38.0 35.0 39.0 26.0 39.0 13 34.36113645232901 39.0 35.0 39.0 27.0 39.0 14 35.453474163686494 40.0 36.0 41.0 27.0 41.0 15 35.044153002667755 39.0 35.0 41.0 24.0 41.0 16 35.129353660331006 39.0 35.0 41.0 25.0 41.0 17 35.36757873726097 39.0 36.0 41.0 27.0 41.0 18 35.42251664950003 40.0 36.0 41.0 27.0 41.0 19 34.82603143732687 39.0 34.0 41.0 19.0 41.0 20 35.10275924279161 40.0 35.0 41.0 25.0 41.0 21 35.09822341357934 40.0 36.0 41.0 24.0 41.0 22 35.123360480068584 40.0 36.0 41.0 25.0 41.0 23 35.13252426252791 40.0 36.0 41.0 25.0 41.0 24 33.87539968396338 39.0 34.0 41.0 10.0 41.0 25 34.459830722086096 39.0 34.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.16204252764794447 1101 2 -0.44704661525656775 1101 3 -1.167955663822788 1101 4 -0.5189392292798019 1101 5 -0.38672437617714905 1101 6 -2.279701706688499 1101 7 -0.6810484528201215 1101 8 -0.05070732426689872 1101 9 -0.31178837685411054 1101 10 -9.630792335434723 1101 11 -4.216174155089185 1101 12 -1.5654376825175333 1101 13 -0.8861141759197366 1101 14 -0.7323026427887953 1101 15 -2.320616794122408 1101 16 -0.6063102464466468 1101 17 -0.5051572975378349 1101 18 -0.30087999194584114 1101 19 -1.1261374488648173 1101 20 -0.5017941800956933 1101 21 -0.5885911048079251 1101 22 -0.3793904968315971 1101 23 -0.36058251503612837 1101 24 -0.4811090409866523 1101 25 -0.12268531252592396 1102 1 -0.09347362289173589 1102 2 -0.019059914360656904 1102 3 -0.4034442740112034 1102 4 -0.09973987460533351 1102 5 -0.09452441114250831 1102 6 -0.9230059942386823 1102 7 -0.20134369538461527 1102 8 0.4657631990172675 1102 9 0.41069404078560723 1102 10 0.23447142297306556 1102 11 0.06175595713734339 1102 12 0.1282749942645438 1102 13 -0.0717970892087223 1102 14 -0.07094365592534047 1102 15 0.43840442126111867 1102 16 0.2161792256071422 1102 17 0.09296806860158568 1102 18 -0.15431687180917208 1102 19 0.25654611158997653 1102 20 0.30715081694452095 1102 21 -0.07208530945340641 1102 22 0.06606354672787518 1102 23 -0.30865435964272336 1102 24 -3.266665588249694 1102 25 -1.7195899583176626 1103 1 0.21506525353999706 1103 2 0.015810699541532358 1103 3 0.3389198251343011 1103 4 0.34391808551829683 1103 5 0.20817542523104748 1103 6 0.694126300773739 1103 7 0.6292256640481426 1103 8 0.6839469454568814 1103 9 0.6297428863101189 1103 10 0.6992311345057018 1103 11 0.40085358758398115 1103 12 0.4315202521277044 1103 13 0.5109009290932036 1103 14 0.257484924160714 1103 15 0.7215463520531955 1103 16 0.5712864918334262 1103 17 0.2589231622322785 1103 18 0.31344720309537877 1103 19 -1.3189084063180303 1103 20 -0.02594414699706249 1103 21 0.1505284715295616 1103 22 0.12977180683332534 1103 23 -0.07322468871369381 1103 24 1.5453442899395071 1103 25 0.7318281375553184 1104 1 -0.4207560003239337 1104 2 -0.40746291169462623 1104 3 -1.4312326312745682 1104 4 -0.4249603995211686 1104 5 -0.5061293675422789 1104 6 -1.55230387839255 1104 7 -0.43047317452212397 1104 8 0.3152380231556293 1104 9 0.039543762634359325 1104 10 0.03696109459092867 1104 11 -0.18265080904276942 1104 12 -0.05894672065222295 1104 13 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1.498230657259512 1110 9 1.2150877138963239 1110 10 1.2122672993501098 1110 11 0.7850559985736112 1110 12 1.0262917688083917 1110 13 0.8417111337469692 1110 14 0.8635684677547673 1110 15 1.3984527159444156 1110 16 0.8978143864227164 1110 17 0.6167939219743559 1110 18 0.7102722661735967 1110 19 -1.4185649804699807 1110 20 0.3241539746738127 1110 21 0.283987961296873 1110 22 0.5122921320365776 1110 23 0.3979625835038618 1110 24 -0.2671942578597637 1110 25 -0.02966611531824981 1111 1 0.5413332448436243 1111 2 0.28866481518123877 1111 3 0.8155345704352364 1111 4 0.747897500439727 1111 5 0.8207755298076478 1111 6 1.0784976027524422 1111 7 0.9112570645490123 1111 8 1.2779489269082944 1111 9 0.8695968918691932 1111 10 0.6377923806184782 1111 11 0.6192769577025956 1111 12 0.5520186421984334 1111 13 0.5453369034444151 1111 14 0.42711092520647753 1111 15 -0.6335634533792884 1111 16 -0.0711200084280108 1111 17 0.20739923345444566 1111 18 0.3906199196102307 1111 19 0.9540853741261444 1111 20 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-2.7038429761893212 2216 14 -2.8323127389911846 2216 15 -3.99630579437547 2216 16 -2.9311326122884367 2216 17 -3.302427520427898 2216 18 -2.9554567840545474 2216 19 -2.2193352308471646 2216 20 -3.1320712487354463 2216 21 -5.661841019908039 2216 22 -3.960949455375541 2216 23 -4.2132717106572635 2216 24 -4.113131965922044 2216 25 -3.7522556469986874 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33219.0 3 2.0 4 245.0 5 1610.0 6 2235.0 7 1799.0 8 1379.0 9 1327.0 10 1220.0 11 1257.0 12 1102.0 13 1218.0 14 1189.0 15 1174.0 16 1237.0 17 1382.0 18 1658.0 19 1971.0 20 2533.0 21 3465.0 22 4246.0 23 5130.0 24 6275.0 25 7157.0 26 8281.0 27 9024.0 28 10251.0 29 12082.0 30 14733.0 31 17974.0 32 23032.0 33 31341.0 34 43088.0 35 60777.0 36 90061.0 37 149251.0 38 227630.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.30717447093391 18.637387892879907 12.306421639479662 25.74901599670652 2 13.98939032828477 20.25165936290715 42.915609543579095 22.843340765228977 3 19.06660208407014 24.470525220529066 29.36573226925817 27.097140426142623 4 11.136705570529523 17.040148018207777 39.714079015602096 32.10906739566061 5 12.071134499541204 39.10324254248502 36.55062177419045 12.27500118378332 6 30.585970893417493 38.390896386504295 19.10202660851493 11.921106111563288 7 27.311917103765314 31.333780805989804 23.327014814082812 18.02728727616207 8 23.666403598262463 38.69505238911025 21.172001822229923 16.466542190397362 9 25.81457162126019 15.741922108768232 20.620841720809345 37.82266454916223 10 13.422817932928787 30.151710826595533 36.04466465034066 20.38080659013502 11 34.01036794561883 22.984924121533513 24.987491154230547 18.017216778617104 12 22.48174509939419 25.323315852650385 33.01404853128991 19.180890516665514 13 28.56894235075821 22.675922963721288 26.14114786614627 22.61398681937424 14 19.54222738650382 23.21316141053138 28.59159291355914 28.653018289405658 15 22.188808853206254 32.52219891817464 23.42227403427188 21.866718194347225 16 20.672727458869538 29.386268005774134 29.294383529387673 20.64662100596865 17 20.55910676008574 29.571871900694248 28.890296573567518 20.978724765652494 18 20.174570898793153 28.883025213094992 30.9714265239763 19.970977364135546 19 22.76110520994638 27.694109215628558 28.006629212045205 21.538156362379862 20 22.3656478145975 28.586126805172533 29.170052531560525 19.878172848669433 21 21.873584369649397 27.854614603034854 27.83490774888861 22.436893278427135 22 21.55308546551358 29.517632876824525 28.356568275410204 20.572713382251695 23 21.157540057816856 28.957568979042346 28.563677244242307 21.321213718898484 24 22.126132674384827 27.939706723552586 29.412743282653846 20.521417319408737 25 21.517591039210494 28.350666518224475 29.101707213138667 21.03003522942636 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 198.0 1 198.0 2 291.5 3 385.0 4 385.0 5 385.0 6 586.5 7 788.0 8 788.0 9 788.0 10 1300.5 11 1813.0 12 1813.0 13 1813.0 14 2992.5 15 4172.0 16 4172.0 17 4172.0 18 7329.5 19 10487.0 20 10487.0 21 10487.0 22 16501.5 23 22516.0 24 22516.0 25 22516.0 26 32094.0 27 41672.0 28 41672.0 29 41672.0 30 54494.0 31 67316.0 32 67316.0 33 67316.0 34 76207.5 35 85099.0 36 85099.0 37 85099.0 38 91035.5 39 96972.0 40 96972.0 41 96972.0 42 100076.5 43 103181.0 44 103181.0 45 103181.0 46 103697.5 47 104214.0 48 104214.0 49 104214.0 50 97293.0 51 90372.0 52 90372.0 53 90372.0 54 77385.5 55 64399.0 56 64399.0 57 64399.0 58 53500.5 59 42602.0 60 42602.0 61 42602.0 62 33716.5 63 24831.0 64 24831.0 65 24831.0 66 18637.0 67 12443.0 68 12443.0 69 12443.0 70 8818.0 71 5193.0 72 5193.0 73 5193.0 74 3565.5 75 1938.0 76 1938.0 77 1938.0 78 1305.5 79 673.0 80 673.0 81 673.0 82 439.0 83 205.0 84 205.0 85 205.0 86 135.5 87 66.0 88 66.0 89 66.0 90 41.5 91 17.0 92 17.0 93 17.0 94 8.5 95 0.0 96 0.0 97 0.0 98 1.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23440448848769438 2 0.14522330482179757 3 0.148294105981025 4 0.07293152753165165 5 0.020727907824785202 6 0.019320457293472627 7 0.3024739141839026 8 0.293005610609618 9 0.016889406375750905 10 0.034034712848104096 11 0.013818605216523469 12 0.015481955844438332 13 0.01343475507162004 14 0.015226055747836044 15 0.012539104733512036 16 0.01778505671385891 17 0.01663350627914862 18 0.01228320463690975 19 0.021111757969688633 20 0.01535400579613719 21 0.012922954878415467 22 0.014970155651233759 23 0.015609905892739475 24 0.013818605216523469 25 0.013562705119921182 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 781555.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.83110325210711 #Duplication Level Percentage of deduplicated Percentage of total 1 93.50425876978682 80.25573688981315 2 5.0203550638193235 8.618052276898302 3 0.771391318528391 1.986281037251681 4 0.2306760520596502 0.7919672016848109 5 0.12061141162084715 0.5176105262105662 6 0.0725456137606628 0.37360020391073595 7 0.04750851834874233 0.285439598062187 8 0.03645251100873032 0.25030073889511223 9 0.02304907039953046 0.17804944271944664 >10 0.1454345122019662 2.341265042442773 >50 0.014306542345564324 0.85084198063888 >100 0.011622534011181209 2.0807110399746276 >500 0.0010430478967442733 0.5938128483526788 >1k 7.450342119601953E-4 0.8763311731450689 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1809 0.23146163737676811 No Hit CTGTAGGACGTGGAATATGGCAAGA 1390 0.1778505671385891 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1323 0.1692779139024125 No Hit GTCCTACAGTGTGCATTTCTCATTT 1253 0.16032141052133247 No Hit GTATCAACGCAGAGTACTTTTTTTT 1077 0.13780220202033125 No Hit CTTTAGGACGTGAAATATGGCGAGG 942 0.12052894549967692 No Hit CTGTAGGACCTGGAATATGGCGAGA 847 0.10837369091106833 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.2795004830114324E-4 0.0 3 0.0 0.0 0.0 2.559000966022865E-4 0.0 4 0.0 0.0 0.0 2.559000966022865E-4 0.0 5 0.0 0.0 0.0 2.559000966022865E-4 0.0 6 0.0 0.0 0.0 2.559000966022865E-4 0.0 7 0.0 0.0 0.0 2.559000966022865E-4 0.0 8 0.0 0.0 0.0 2.559000966022865E-4 0.0 9 0.0 0.0 0.0 2.559000966022865E-4 0.0 10 0.0 0.0 0.0 2.559000966022865E-4 0.0 11 0.0 0.0 0.0 2.559000966022865E-4 0.0 12 0.0 0.0 0.0 2.559000966022865E-4 0.0 13 0.0 0.0 0.0 2.559000966022865E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATAGAC 50 8.4278334E-5 15.257771 3 TCCAACG 85 1.7575076E-8 14.500638 18 GCCTAAT 50 0.0014476837 13.360868 1 CTACACT 160 0.0 12.499247 4 TAGGACC 315 0.0 12.395323 4 CCAACGA 70 1.1099099E-4 12.190097 19 ATTAACC 55 0.0029801787 12.136863 3 TCTACAC 150 1.2732926E-11 12.079069 3 GTCCTAT 120 9.325959E-9 11.929347 1 TTTAGAC 80 2.75305E-5 11.920135 3 GTAGGAC 820 0.0 11.861988 3 CTGACCC 75 2.1060248E-4 11.379608 9 ATACACT 285 0.0 11.361053 4 ACTGTTC 260 0.0 11.339321 8 TGTAGGA 895 0.0 11.294161 2 CACTGTT 310 0.0 11.053557 7 GCCGGTT 95 1.3878429E-5 10.978216 11 GTCCTAC 840 0.0 10.906833 1 GTCTAAT 70 0.0014342504 10.906832 1 TAAGACA 140 8.005372E-9 10.8836975 4 >>END_MODULE