FastQCFastQC Report
Thu 26 May 2016
SRR1926746_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926746_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781555
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19190.24553614268989385No Hit
GTCCTACAGTGGACATTTCTAAATT17340.22186538375418238No Hit
CTGTAGGACGTGGAATATGGCAAGA15710.201009525881096No Hit
GTCCTACAGTGTGCATTTCTCATTT12960.16582326259828162No Hit
GTCCTAAAGTGTGTATTTCTCATTT12670.1621127111975485No Hit
CTTTAGGACGTGAAATATGGCGAGG11760.15046925680214446No Hit
GGTATCAACGCAGAGTACTTTTTTT11230.14368790424218383No Hit
TATCAACGCAGAGTACTTTTTTTTT9710.12423949690041008No Hit
CTGTAGGACCTGGAATATGGCGAGA9600.12283204636909752No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTTG400.00517921914.2898085
TCCAACG759.813302E-713.91324918
TAGGACC3350.013.3655924
TAGGATA905.213951E-712.7020524
GTCCTAG1251.3078534E-912.2151641
GTAGGAC8100.012.1155513
GATATAC2800.011.928871
TCTGGAC802.77784E-511.9097043
GTATAGG1302.4265319E-911.7453491
TGTAGGA9200.011.7191492
GAGGTGT907.219349E-611.6435486
TAAGCAG907.219349E-611.6435485
AATCCCG1552.5465852E-1111.62837619
GGCGAGG3200.011.56143619
AGGACCT6250.011.2794235
GGACCTG5850.011.0735856
TGGCGAG8350.010.90641218
CTGTAGG8300.010.8078441
AGGACGT7200.010.7173575
GTCCTAC9350.010.71684551
Produced by FastQC (version 0.11.5)