##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926746_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 781555 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.12145658334986 31.0 30.0 33.0 26.0 34.0 2 30.443985388104483 31.0 30.0 34.0 27.0 34.0 3 30.490038448989516 31.0 31.0 34.0 26.0 34.0 4 34.07479192123395 37.0 35.0 37.0 30.0 37.0 5 34.06819353724306 37.0 35.0 37.0 30.0 37.0 6 34.06596464740166 37.0 35.0 37.0 30.0 37.0 7 34.06136356366475 37.0 35.0 37.0 30.0 37.0 8 34.09408550901728 37.0 35.0 37.0 30.0 37.0 9 35.64675806565117 39.0 37.0 39.0 30.0 39.0 10 35.55194580035954 39.0 35.0 39.0 30.0 39.0 11 35.5947207810071 39.0 37.0 39.0 30.0 39.0 12 35.49553390356405 39.0 35.0 39.0 30.0 39.0 13 35.52610244960368 39.0 35.0 39.0 30.0 39.0 14 36.6506797346316 40.0 37.0 41.0 30.0 41.0 15 36.66042952831215 40.0 37.0 41.0 30.0 41.0 16 36.63948922340718 40.0 37.0 41.0 30.0 41.0 17 36.61855147750318 40.0 37.0 41.0 30.0 41.0 18 36.59133906123049 40.0 37.0 41.0 30.0 41.0 19 36.665047245555336 40.0 37.0 41.0 30.0 41.0 20 36.635028884723404 40.0 37.0 41.0 30.0 41.0 21 36.57736819545649 40.0 37.0 41.0 30.0 41.0 22 36.50342842154423 40.0 37.0 41.0 30.0 41.0 23 36.440726500374254 40.0 37.0 41.0 30.0 41.0 24 36.38321295366289 40.0 37.0 41.0 30.0 41.0 25 36.27726007766568 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.10608089940082976 1101 2 -0.24813404266386385 1101 3 -0.05363856006730927 1101 4 -0.08311803256698624 1101 5 -0.07010876450203085 1101 6 0.10925880199983595 1101 7 -0.08916450668586151 1101 8 -0.013228921974608454 1101 9 0.018795536434616622 1101 10 -0.037693647654450047 1101 11 -0.021114509608459286 1101 12 -0.05849667516077517 1101 13 -0.3149172797995732 1101 14 -0.20484121063172012 1101 15 -0.25931579982920283 1101 16 -0.2641798672035165 1101 17 -0.13420070466465717 1101 18 -0.1155075788195461 1101 19 -0.3194472954124947 1101 20 -0.20246753983628452 1101 21 -0.2730573469234585 1101 22 -0.5662461544814974 1101 23 -0.33523920306507193 1101 24 -0.37671173353999876 1101 25 -0.3859661260779177 1102 1 -1.0456199701197981 1102 2 -0.8948290496722713 1102 3 -0.669507311159979 1102 4 -0.8154168207413406 1102 5 -0.7892510704366344 1102 6 -0.7633814198154809 1102 7 -0.7558281062159722 1102 8 -0.7142812565255952 1102 9 -0.6894783711280255 1102 10 -0.7224732892288728 1102 11 -0.8558081180025994 1102 12 -0.6215984090130746 1102 13 -0.7029146543306837 1102 14 -0.6668823286643359 1102 15 -0.6662604104102101 1102 16 -0.6308560419934963 1102 17 -0.8885169325774385 1102 18 -0.9867261828430145 1102 19 -0.9545055863426626 1102 20 -1.0231243978002595 1102 21 -0.976926846397447 1102 22 -0.8340991895664729 1102 23 -0.9235865378767656 1102 24 -0.7337540324201868 1102 25 -0.8264498748549016 1103 1 -0.26095843445529354 1103 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0.46886169830420243 2214 6 0.34045065598033375 2214 7 0.33884530842784244 2214 8 0.3599619085047294 2214 9 0.16267618045304033 2214 10 -0.04790241564772657 2214 11 0.09487835212703288 2214 12 0.2592663187113331 2214 13 0.1741378186796112 2214 14 0.1985153483048947 2214 15 0.1296828748680099 2214 16 0.14588193652045334 2214 17 0.22931279111668346 2214 18 0.2636471141957131 2214 19 0.37003819842681906 2214 20 0.36059456568931125 2214 21 0.2187323367097065 2214 22 0.3756977104163468 2214 23 0.27258270773286597 2214 24 0.3046605453247011 2214 25 0.2638245810564115 2215 1 0.4118781860767875 2215 2 0.3046838649827208 2215 3 0.2619928822553348 2215 4 0.3079192386994052 2215 5 0.4241904237736236 2215 6 0.293929745936758 2215 7 0.17728165061613765 2215 8 0.32475584307459116 2215 9 0.20611684609276892 2215 10 0.24435431147090725 2215 11 0.09238334843348639 2215 12 0.06747744057540217 2215 13 0.10971610178264513 2215 14 0.10914073125961465 2215 15 0.10589702028486414 2215 16 0.006320924317435583 2215 17 0.12177624220441885 2215 18 0.032113405590614263 2215 19 0.08109420859695149 2215 20 0.07357522754959689 2215 21 0.006701116236591531 2215 22 0.12076811682078414 2215 23 0.28805264686096166 2215 24 0.23757432253238164 2215 25 0.17726450226079038 2216 1 -0.19584793936474654 2216 2 -0.041926320511354476 2216 3 -1.5319586044381523 2216 4 -1.2707716882445297 2216 5 -0.4270744229907706 2216 6 -0.3112008243357849 2216 7 -0.24218032101013875 2216 8 -0.2887221607970005 2216 9 -0.18275350947426006 2216 10 -0.39835687636752226 2216 11 -0.22941227677429765 2216 12 -0.2987476116818897 2216 13 -0.33575807055512286 2216 14 -0.3821029894252348 2216 15 -0.44939385527440123 2216 16 -0.3054707125703473 2216 17 -0.3265189369638506 2216 18 -0.36882719705197786 2216 19 -0.3256623602576454 2216 20 -0.2960167221094494 2216 21 -0.3062423840892805 2216 22 -0.22974169576251313 2216 23 -0.5558661378973611 2216 24 -0.5034852888324437 2216 25 -0.5922918557907835 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16282.0 3 15.0 4 255.0 5 1672.0 6 2299.0 7 1689.0 8 1419.0 9 1206.0 10 971.0 11 908.0 12 815.0 13 894.0 14 845.0 15 895.0 16 892.0 17 905.0 18 1018.0 19 1282.0 20 1617.0 21 1968.0 22 2711.0 23 3319.0 24 3962.0 25 4602.0 26 5749.0 27 6355.0 28 7362.0 29 9049.0 30 11377.0 31 14969.0 32 19808.0 33 25983.0 34 35425.0 35 50154.0 36 74907.0 37 132284.0 38 335692.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.025274295925755 19.437381106406963 10.497735817635641 28.03960878003164 2 13.796138183001394 21.077173569112638 41.021012865464115 24.105675382421854 3 18.490714778417793 24.972389407258483 29.382149799783207 27.154746014540514 4 11.556064512414354 17.60605459628561 38.835270710314695 32.00261018098534 5 12.392340518096262 39.02625029589963 36.04358201697984 12.537827169024268 6 30.986166855405507 39.08807042141403 17.87857544546218 12.047187277718288 7 27.785321305210836 32.08905656707077 21.916172569174716 18.209449558543675 8 24.30065120278652 39.3544531700869 19.939166475788795 16.405729151337788 9 25.922357180514823 15.935742108522847 20.036315894538028 38.105584816424305 10 13.601501681330324 30.7697524324269 35.053434059606616 20.57531182663616 11 34.13450711434128 23.168185075238 24.63468625243116 18.06262155798956 12 22.70489307200782 25.543468525527345 32.450093019804136 19.3015453826607 13 28.820457834377628 22.73241984827567 25.843677107897268 22.60344520944944 14 19.707250289486986 23.435586746933996 28.208763298808144 28.648399664770874 15 22.01807980448732 32.620675977403444 23.433883319365094 21.927360898744137 16 20.901131212166575 29.593334280168538 28.865934532743225 20.639599974921662 17 20.524723148082987 29.89898343686625 28.551157628062008 21.02513578698876 18 20.20479763342682 28.975039408766147 30.759949433946815 20.060213523860217 19 22.419436149005698 27.936393390603847 28.15685160590311 21.48731885448734 20 22.353371692954138 28.60030887299741 29.064386073333665 19.981933360714784 21 21.780803511443544 27.81884841543551 27.862101619435787 22.538246453685158 22 21.735386505108405 29.42582415824862 28.135959721324795 20.70282961531818 23 21.07682760650242 29.092770182520745 28.500873259079658 21.329528951897178 24 21.97506506268281 27.924621971098606 29.49495492314031 20.60535804307828 25 21.552991576574097 28.509259626543965 28.856663565835927 21.08108523104601 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 281.0 1 281.0 2 274.0 3 267.0 4 267.0 5 267.0 6 468.5 7 670.0 8 670.0 9 670.0 10 1175.5 11 1681.0 12 1681.0 13 1681.0 14 2741.0 15 3801.0 16 3801.0 17 3801.0 18 6710.0 19 9619.0 20 9619.0 21 9619.0 22 15270.5 23 20922.0 24 20922.0 25 20922.0 26 30522.5 27 40123.0 28 40123.0 29 40123.0 30 53072.0 31 66021.0 32 66021.0 33 66021.0 34 75041.0 35 84061.0 36 84061.0 37 84061.0 38 90141.5 39 96222.0 40 96222.0 41 96222.0 42 100057.0 43 103892.0 44 103892.0 45 103892.0 46 104903.5 47 105915.0 48 105915.0 49 105915.0 50 99033.5 51 92152.0 52 92152.0 53 92152.0 54 78988.5 55 65825.0 56 65825.0 57 65825.0 58 54808.5 59 43792.0 60 43792.0 61 43792.0 62 34573.0 63 25354.0 64 25354.0 65 25354.0 66 19025.5 67 12697.0 68 12697.0 69 12697.0 70 9008.5 71 5320.0 72 5320.0 73 5320.0 74 3646.5 75 1973.0 76 1973.0 77 1973.0 78 1334.5 79 696.0 80 696.0 81 696.0 82 443.0 83 190.0 84 190.0 85 190.0 86 128.0 87 66.0 88 66.0 89 66.0 90 40.0 91 14.0 92 14.0 93 14.0 94 7.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11771404443705177 2 0.12001714530647237 3 0.01944840734177377 4 0.0 5 0.005118001932045729 6 0.34559308046138787 7 0.02098380792138749 8 0.009980103767489172 9 0.010108053815790316 10 0.00396645149733544 11 0.004478251690540013 12 1.2795004830114324E-4 13 7.677002898068593E-4 14 0.0 15 0.0025590009660228646 16 5.11800193204573E-4 17 0.0 18 3.838501449034297E-4 19 1.2795004830114324E-4 20 5.11800193204573E-4 21 0.014074505313125757 22 0.0 23 0.0 24 1.2795004830114324E-4 25 0.005629802125250302 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 781555.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.4189669242642 #Duplication Level Percentage of deduplicated Percentage of total 1 92.00593043047819 76.75039667416213 2 6.2248164121810685 10.38535508794699 3 0.9121405246648893 2.282694607719042 4 0.2970467212715338 0.9911732246684483 5 0.15372071117940317 0.6411611460724502 6 0.08899011646700122 0.44540781492883164 7 0.05487701253828398 0.32044485856834803 8 0.04032195003272909 0.26908923328816503 9 0.031015490380552346 0.23285521495756178 >10 0.16013553788494078 2.5095792517951745 >50 0.016577812954351585 0.9534165701329379 >100 0.012125054462044533 2.183216150697582 >500 0.0012278536026470437 0.7438708517244679 >1k 0.0010743719023161632 1.2913393133378628 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1919 0.24553614268989385 No Hit GTCCTACAGTGGACATTTCTAAATT 1734 0.22186538375418238 No Hit CTGTAGGACGTGGAATATGGCAAGA 1571 0.201009525881096 No Hit GTCCTACAGTGTGCATTTCTCATTT 1296 0.16582326259828162 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1267 0.1621127111975485 No Hit CTTTAGGACGTGAAATATGGCGAGG 1176 0.15046925680214446 No Hit GGTATCAACGCAGAGTACTTTTTTT 1123 0.14368790424218383 No Hit TATCAACGCAGAGTACTTTTTTTTT 971 0.12423949690041008 No Hit CTGTAGGACCTGGAATATGGCGAGA 960 0.12283204636909752 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGTTG 40 0.005179219 14.289808 5 TCCAACG 75 9.813302E-7 13.913249 18 TAGGACC 335 0.0 13.365592 4 TAGGATA 90 5.213951E-7 12.702052 4 GTCCTAG 125 1.3078534E-9 12.215164 1 GTAGGAC 810 0.0 12.115551 3 GATATAC 280 0.0 11.92887 1 TCTGGAC 80 2.77784E-5 11.909704 3 GTATAGG 130 2.4265319E-9 11.745349 1 TGTAGGA 920 0.0 11.719149 2 GAGGTGT 90 7.219349E-6 11.643548 6 TAAGCAG 90 7.219349E-6 11.643548 5 AATCCCG 155 2.5465852E-11 11.628376 19 GGCGAGG 320 0.0 11.561436 19 AGGACCT 625 0.0 11.279423 5 GGACCTG 585 0.0 11.073585 6 TGGCGAG 835 0.0 10.906412 18 CTGTAGG 830 0.0 10.807844 1 AGGACGT 720 0.0 10.717357 5 GTCCTAC 935 0.0 10.7168455 1 >>END_MODULE