FastQCFastQC Report
Thu 26 May 2016
SRR1926745_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926745_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654171
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT19930.30466040224956475No Hit
CTGTAGGACGTGGAATATGGCAAGA15660.2393869492839028No Hit
GTCCTAAAGTGTGTATTTCTCATTT15610.23862262313676394No Hit
GTCCTACAGTGTGCATTTCTCATTT13930.21294126459289697No Hit
CTTTAGGACGTGAAATATGGCGAGG11710.1790051836599299No Hit
CTGTAGGACCTGGAATATGGCGAGA8580.1311583668490349No Hit
GTATCAACGCAGAGTACTTTTTTTT7780.11892914849481251No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA456.857797E-414.74834419
TCCAACG653.435247E-614.58627418
CTAGGAC801.2314558E-714.2992363
TAGGACC2800.013.9437714
CTAGACA551.918384E-413.8510194
TCAGACT707.0715414E-613.6036794
TGTCGAG500.001520600913.2735118
CCTGCAC751.4240477E-512.7104313
TAGGACA2350.012.5616944
GGGCTAC853.7489262E-612.3470351
CTTATCC802.7611512E-511.916033
GGACCTG5600.011.7304716
ATGGCAA6600.011.49221617
TTAGGAC4500.011.4393873
AGGACGT8100.011.400955
GGCAAGA6350.011.34743219
TGGCAAG6650.011.26323518
AGGACCT5750.011.2588875
GGACGTG7950.011.2568046
GGCGAGG3650.011.16948919
Produced by FastQC (version 0.11.5)