FastQCFastQC Report
Thu 26 May 2016
SRR1926745_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926745_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654171
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT20220.30909349390297036No Hit
CTGTAGGACGTGGAATATGGCAAGA18310.2798962350822644No Hit
GTCCTAAAGTGTGTATTTCTCATTT15520.2372468360719139No Hit
CTTTAGGACGTGAAATATGGCGAGG15210.23250801395965276No Hit
GTCCTACAGTGTGCATTTCTCATTT14310.21875014331115258No Hit
GTATCAACGCAGAGTACTTTTTTTT12640.1932216499967134No Hit
CTGTAGGACCTGGAATATGGCGAGA11100.16968040466483536No Hit
CTGAAGGACCTGGAATATGGCGAGA8350.12764246657219594No Hit
GGTATCAACGCAGAGTACTTTTTTT7320.11189734794113466No Hit
TATCAACGCAGAGTACTTTTTTTTT7300.1115916174822791No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGCC551.14110935E-515.52406311
TGCGCCA551.14193645E-515.52287612
GCGTGCG400.005317783414.2325689
CGTGCGC400.00532048214.2314810
GCGCCAC602.5991536E-514.22930413
TCCAACG954.960384E-913.98394218
TAGGACC3500.013.8768524
ATAAACC551.9170997E-413.8521153
AGGCGTG707.327788E-613.5568997
GGCGTGC500.001512679513.2827148
CTAGGAC1102.6357156E-912.9863583
GGCGAGG3900.012.89544919
GTCTAAT603.9742538E-412.7084991
AAGGCGT604.0026795E-412.6977726
AAAGGCG751.4387235E-512.6977715
GTCTAGA550.002990384812.130841
GTCCTAC9000.012.0730751
GTATAGA1052.5968075E-711.8007491
TGAACTG657.849272E-411.721025
GACGAAA658.105612E-411.67621911