##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926745_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 654171 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.944551195329662 31.0 30.0 33.0 26.0 34.0 2 30.244922199241483 31.0 30.0 34.0 26.0 34.0 3 30.331355257264537 31.0 30.0 34.0 26.0 34.0 4 33.915454827560374 37.0 35.0 37.0 30.0 37.0 5 33.88903665861067 37.0 35.0 37.0 30.0 37.0 6 33.893419304738366 37.0 35.0 37.0 30.0 37.0 7 33.89074722052796 37.0 35.0 37.0 30.0 37.0 8 33.924912599305074 37.0 35.0 37.0 30.0 37.0 9 35.47288705858254 39.0 35.0 39.0 30.0 39.0 10 35.368489278797135 39.0 35.0 39.0 30.0 39.0 11 35.40075760007704 39.0 35.0 39.0 30.0 39.0 12 35.288114269816305 39.0 35.0 39.0 30.0 39.0 13 35.301364627903105 39.0 35.0 39.0 30.0 39.0 14 36.415793424043564 40.0 37.0 41.0 30.0 41.0 15 36.425503423416814 40.0 37.0 41.0 30.0 41.0 16 36.41524463786992 40.0 37.0 41.0 30.0 41.0 17 36.39574056324722 40.0 37.0 41.0 30.0 41.0 18 36.374270641774096 40.0 37.0 41.0 30.0 41.0 19 36.43488934850368 40.0 37.0 41.0 30.0 41.0 20 36.40692265477987 40.0 37.0 41.0 30.0 41.0 21 36.343981925215274 40.0 37.0 41.0 29.0 41.0 22 36.26722829351959 40.0 37.0 41.0 29.0 41.0 23 36.20241802219909 40.0 37.0 41.0 29.0 41.0 24 36.13902481155539 40.0 37.0 41.0 29.0 41.0 25 36.01591327038343 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.04145917605788796 1101 2 0.04910067762554604 1101 3 0.29503862644145684 1101 4 0.011110822327587755 1101 5 0.09035173941921215 1101 6 0.1307117534432578 1101 7 0.23078009436344615 1101 8 0.15568922131209462 1101 9 0.3147148135185134 1101 10 0.3231918571017047 1101 11 0.32681992345409583 1101 12 0.18051531153893308 1101 13 0.22344909032491955 1101 14 0.2395266288632243 1101 15 0.1827704004662536 1101 16 0.1215495441181389 1101 17 0.30080650702058165 1101 18 0.12231059671133693 1101 19 0.2294471420578006 1101 20 0.2639468117170267 1101 21 0.3190976232037883 1101 22 0.130270914240036 1101 23 0.25412786279584054 1101 24 0.25661628317994456 1101 25 0.3052617317264108 1102 1 -0.6418367126317435 1102 2 -0.5367826006503726 1102 3 -0.30001249830291954 1102 4 -0.31538595288084537 1102 5 -0.46872958825107247 1102 6 -0.19534456242900688 1102 7 -0.3175917791618019 1102 8 -0.06358716692134436 1102 9 -0.08087430084360392 1102 10 -0.15266467900023173 1102 11 -0.32226926956147395 1102 12 -0.16786600040065736 1102 13 -0.1113471972320994 1102 14 -0.04876659120913729 1102 15 -0.1671336423212253 1102 16 0.006268751291329977 1102 17 0.05062245794082543 1102 18 -0.12741726234168027 1102 19 -0.25574398583271574 1102 20 -0.22633476764430327 1102 21 -0.23792298400086764 1102 22 -0.21009089164324024 1102 23 -0.10559213091188013 1102 24 -0.03458082237298754 1102 25 -0.11028962897832884 1103 1 -0.6176292654027122 1103 2 -0.5785735195635944 1103 3 -0.43462024068044514 1103 4 -0.4591432986525774 1103 5 -0.7196933007975232 1103 6 -0.5453843547709596 1103 7 -0.5466480945490488 1103 8 -0.551050207477914 1103 9 -0.405907636812195 1103 10 -0.35060074772766825 1103 11 -0.4475239669025015 1103 12 -0.42460935297135194 1103 13 -0.5426118558348918 1103 14 -0.4365369024326853 1103 15 -0.436887773082546 1103 16 -0.3823880595237483 1103 17 -0.15710621626266885 1103 18 -0.29544319324530477 1103 19 -0.20094228872886077 1103 20 -0.22187802534583767 1103 21 -0.40117488797219636 1103 22 -0.4134988026770543 1103 23 -0.7097758608510105 1103 24 -0.49075812229128957 1103 25 -0.4373942749318971 1104 1 -0.3271100950538006 1104 2 -0.23706877747422794 1104 3 0.16017758995234388 1104 4 -0.02204008625595577 1104 5 -0.05309117371245975 1104 6 -0.16575137218408997 1104 7 -0.3521627750362555 1104 8 -0.407726286457617 1104 9 -0.45113802218675403 1104 10 -0.244750949823775 1104 11 -0.2748610498110935 1104 12 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2214 1 0.388862714982384 2214 2 0.34259382449737785 2214 3 0.3772167817938765 2214 4 0.41657365436007154 2214 5 0.336550322400889 2214 6 0.3122630507684363 2214 7 0.1899934970260162 2214 8 0.17429640141594405 2214 9 0.18758956438976782 2214 10 -0.26239337740406654 2214 11 -0.005641227307300767 2214 12 0.12071513413329171 2214 13 0.10362790630924223 2214 14 0.10421214209627294 2214 15 0.1726066899417944 2214 16 -0.07825449315931365 2214 17 0.18594006229654525 2214 18 0.08816000975102156 2214 19 0.017894619965602487 2214 20 -0.001617935776835111 2214 21 -0.051806366196409215 2214 22 -0.019837273513033438 2214 23 0.23269629572499895 2214 24 -0.0017835772708423292 2214 25 -0.0020100440744243997 2215 1 0.23289052266641264 2215 2 0.12456189487788905 2215 3 0.13892892304706805 2215 4 0.18411495061649674 2215 5 0.3597285192079269 2215 6 0.4032070970325492 2215 7 0.22892117859112204 2215 8 0.22723558748749184 2215 9 0.2436509627717598 2215 10 0.351528921036369 2215 11 0.08068871209410133 2215 12 0.12760553371494154 2215 13 0.12353008724375769 2215 14 0.27231943546119197 2215 15 0.2506773780589313 2215 16 -9.528228526320959E-4 2215 17 -0.10034988817870527 2215 18 0.09266645412026975 2215 19 -0.058161241488150495 2215 20 0.06576340477501219 2215 21 0.29251366825559444 2215 22 0.34375434726890575 2215 23 0.15361050409806865 2215 24 0.12761135951589608 2215 25 0.20364069879678226 2216 1 -0.22666169691176918 2216 2 -0.0847012759804926 2216 3 -1.8701124074763982 2216 4 -1.2906979070483828 2216 5 -0.6632576396728567 2216 6 -0.2924478950636811 2216 7 -0.36307490638879614 2216 8 -0.33970258647001117 2216 9 -0.24961320952801458 2216 10 -0.29773000422904516 2216 11 -0.5920352166026248 2216 12 -0.4048226132700776 2216 13 -0.562671168447082 2216 14 -0.7772567749581754 2216 15 -0.8304774556377907 2216 16 -0.5584234865344229 2216 17 -0.745417505728561 2216 18 -0.9168618232303842 2216 19 -0.7028731690926833 2216 20 -0.8059840375388063 2216 21 -0.612874426745222 2216 22 -0.42198776860823983 2216 23 -0.5589450268805791 2216 24 -0.5757140578931441 2216 25 -0.6523534870094849 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15020.0 3 8.0 4 232.0 5 1608.0 6 2186.0 7 1652.0 8 1167.0 9 1128.0 10 1007.0 11 911.0 12 766.0 13 800.0 14 746.0 15 776.0 16 833.0 17 821.0 18 945.0 19 1170.0 20 1418.0 21 1831.0 22 2404.0 23 2938.0 24 3465.0 25 4093.0 26 5007.0 27 5451.0 28 6310.0 29 7738.0 30 10098.0 31 12848.0 32 16552.0 33 22104.0 34 30204.0 35 42695.0 36 63001.0 37 111540.0 38 272698.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.801396991753954 19.486871828149198 10.17976627017894 28.531964909917907 2 13.719269570753445 21.27913881338916 41.2028024682289 23.798789147628494 3 18.365718182249054 25.214620654648417 29.354601482124753 27.065059680977765 4 11.559057188410982 17.825002942655665 38.79750095922931 31.81843890970404 5 12.005852038811797 39.18144836431846 36.34134447075586 12.471355126113883 6 30.882334895820552 39.36302059047292 17.90883377917393 11.845810734532604 7 27.41294795071816 31.80400407318274 22.456477797790328 18.326570178308774 8 23.81692763513947 39.99284515259088 20.124842914407846 16.0653842978618 9 25.989723236767638 15.504581124817879 20.043510366199506 38.462185272214974 10 13.557718178260444 30.607328050092946 35.49524263770668 20.33971113393993 11 34.2213240060171 22.689929922829503 25.20164002592733 17.88710604522607 12 23.0053350046624 25.188865279667365 32.88793432899705 18.91786538667319 13 29.183284441787976 22.874058538389928 25.686634493533017 22.25602252628908 14 19.488817891373802 23.618019780790924 28.007857284803645 28.88530504303163 15 21.605880032897854 33.49924940303847 23.2302880396731 21.66458252439058 16 20.570341042848188 29.132335631410793 29.562499044590858 20.73482428115016 17 20.202362990716495 29.980845986752698 29.00510722731518 20.811683795215625 18 20.128132027044998 28.514955615444936 31.465110697694325 19.891801659815737 19 22.633990195224186 27.574135814641735 27.837064009257517 21.95480998087656 20 22.55973217970864 28.773713254964306 28.84647721540272 19.820077349924333 21 21.52830970432783 27.73835787967325 27.63179419908297 23.10153821691595 22 21.94227503206348 29.99017076574779 27.721497895810117 20.346056306378607 23 20.78022532369262 29.57763272543834 28.43098888668083 21.211153064188206 24 22.300743995071628 27.7934974188706 29.480976686523857 20.424781899533915 25 21.484731511561243 28.761322377221475 29.006841200076437 20.747104911140838 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 208.0 1 208.0 2 220.5 3 233.0 4 233.0 5 233.0 6 410.0 7 587.0 8 587.0 9 587.0 10 969.0 11 1351.0 12 1351.0 13 1351.0 14 2277.0 15 3203.0 16 3203.0 17 3203.0 18 5587.0 19 7971.0 20 7971.0 21 7971.0 22 12743.5 23 17516.0 24 17516.0 25 17516.0 26 25936.0 27 34356.0 28 34356.0 29 34356.0 30 45755.0 31 57154.0 32 57154.0 33 57154.0 34 64579.5 35 72005.0 36 72005.0 37 72005.0 38 76460.5 39 80916.0 40 80916.0 41 80916.0 42 83976.5 43 87037.0 44 87037.0 45 87037.0 46 87517.0 47 87997.0 48 87997.0 49 87997.0 50 81943.5 51 75890.0 52 75890.0 53 75890.0 54 64801.0 55 53712.0 56 53712.0 57 53712.0 58 44717.0 59 35722.0 60 35722.0 61 35722.0 62 28242.0 63 20762.0 64 20762.0 65 20762.0 66 15639.5 67 10517.0 68 10517.0 69 10517.0 70 7543.0 71 4569.0 72 4569.0 73 4569.0 74 3139.5 75 1710.0 76 1710.0 77 1710.0 78 1131.0 79 552.0 80 552.0 81 552.0 82 350.0 83 148.0 84 148.0 85 148.0 86 98.0 87 48.0 88 48.0 89 48.0 90 27.5 91 7.0 92 7.0 93 7.0 94 3.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11724763097110695 2 0.11419032638255135 3 0.016815175237055755 4 0.0 5 0.0061146091771111825 6 0.3392079441002429 7 0.020942536431605804 8 0.010547700830516793 9 0.011312026977655688 10 0.004127361194550048 11 0.005350283029972285 12 1.5286522942777958E-4 13 3.0573045885555917E-4 14 1.5286522942777958E-4 15 0.0038216307356944896 16 1.5286522942777958E-4 17 0.0 18 3.0573045885555917E-4 19 0.0 20 1.5286522942777958E-4 21 0.015592253401633518 22 0.0 23 1.5286522942777958E-4 24 0.0 25 0.007031800553677861 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 654171.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.33533786966197 #Duplication Level Percentage of deduplicated Percentage of total 1 92.93606617366751 77.44858474859845 2 5.5291450741600885 9.215463457710163 3 0.7559309247988933 1.8898727707272542 4 0.24869620849640545 0.8290073024780739 5 0.13612419403273274 0.5671977850976603 6 0.07206316503362042 0.3603244923620381 7 0.05344711075382334 0.3117823122978978 8 0.03413766136340753 0.22758988350397377 9 0.02823452737242258 0.21176404903539495 >10 0.17001009543927023 2.7351065458730064 >50 0.017613861185053203 1.0278030857748577 >100 0.01596234972005168 2.8512744990450556 >500 0.0012843269884153996 0.6834618730154438 >1k 0.0012843269884153996 1.6407671944806999 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2022 0.30909349390297036 No Hit CTGTAGGACGTGGAATATGGCAAGA 1831 0.2798962350822644 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1552 0.2372468360719139 No Hit CTTTAGGACGTGAAATATGGCGAGG 1521 0.23250801395965276 No Hit GTCCTACAGTGTGCATTTCTCATTT 1431 0.21875014331115258 No Hit GTATCAACGCAGAGTACTTTTTTTT 1264 0.1932216499967134 No Hit CTGTAGGACCTGGAATATGGCGAGA 1110 0.16968040466483536 No Hit CTGAAGGACCTGGAATATGGCGAGA 835 0.12764246657219594 No Hit GGTATCAACGCAGAGTACTTTTTTT 732 0.11189734794113466 No Hit TATCAACGCAGAGTACTTTTTTTTT 730 0.1115916174822791 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCGCC 55 1.14110935E-5 15.524063 11 TGCGCCA 55 1.14193645E-5 15.522876 12 GCGTGCG 40 0.0053177834 14.232568 9 CGTGCGC 40 0.005320482 14.23148 10 GCGCCAC 60 2.5991536E-5 14.229304 13 TCCAACG 95 4.960384E-9 13.983942 18 TAGGACC 350 0.0 13.876852 4 ATAAACC 55 1.9170997E-4 13.852115 3 AGGCGTG 70 7.327788E-6 13.556899 7 GGCGTGC 50 0.0015126795 13.282714 8 CTAGGAC 110 2.6357156E-9 12.986358 3 GGCGAGG 390 0.0 12.895449 19 GTCTAAT 60 3.9742538E-4 12.708499 1 AAGGCGT 60 4.0026795E-4 12.697772 6 AAAGGCG 75 1.4387235E-5 12.697771 5 GTCTAGA 55 0.0029903848 12.13084 1 GTCCTAC 900 0.0 12.073075 1 GTATAGA 105 2.5968075E-7 11.800749 1 TGAACTG 65 7.849272E-4 11.72102 5 GACGAAA 65 8.105612E-4 11.676219 11 >>END_MODULE