FastQCFastQC Report
Thu 26 May 2016
SRR1926744_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926744_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630854
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17330.2747069845003757No Hit
GTCCTAAAGTGTGTATTTCTCATTT13900.22033624261715073No Hit
CTGTAGGACGTGGAATATGGCAAGA13360.2117764173643981No Hit
GTCCTACAGTGTGCATTTCTCATTT11110.17611047881126218No Hit
CTTTAGGACGTGAAATATGGCGAGG10030.15899082830575695No Hit
CTGTAGGACCTGGAATATGGCGAGA8070.12792183294391413No Hit
GTATCAACGCAGAGTACTTTTTTTT7830.12411746616491297No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATACT602.5093874E-514.2822494
AAAGGCG551.9291515E-413.841745
CGAAATC551.9885635E-413.79234413
AAGGCGT500.001478710413.3226756
TCTAGGA603.9525836E-412.7165992
GTAGTAC905.141701E-712.7155843
ACGAAAT604.1510523E-412.64298212
TTAGGAC4300.012.6416573
GACGTGA3500.012.5077
ACGTGAA3650.012.2430138
CTTTAGG4650.012.1090071
AGGACGT7100.012.0627845
TTTAGGA4750.012.0473042
TAGGACC3250.012.0117394
GGACGTG6850.011.9473416
CGTGAAA3950.011.7627749
GTACTAA657.710158E-411.7458941
TAAGACT657.8612316E-411.718774
CTACACT1156.825212E-811.5913924
GGCGAGG3150.011.43888919
Produced by FastQC (version 0.11.5)