##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926744_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 630854 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.972337498058188 31.0 30.0 33.0 25.0 34.0 2 29.017473139585388 31.0 30.0 33.0 25.0 34.0 3 28.73239450015376 31.0 29.0 33.0 20.0 34.0 4 32.63200518662004 35.0 33.0 37.0 26.0 37.0 5 32.56627682474868 35.0 33.0 37.0 25.0 37.0 6 32.11312760163207 35.0 32.0 37.0 19.0 37.0 7 32.12301261464618 35.0 32.0 37.0 19.0 37.0 8 31.952466022249205 35.0 32.0 37.0 17.0 37.0 9 34.13759602063235 38.0 34.0 39.0 25.0 39.0 10 34.27218817666211 38.0 35.0 39.0 26.0 39.0 11 34.41667168631728 39.0 35.0 39.0 27.0 39.0 12 34.30549223750662 39.0 35.0 39.0 26.0 39.0 13 34.408164171107735 39.0 35.0 39.0 27.0 39.0 14 35.50061503929594 40.0 36.0 41.0 27.0 41.0 15 35.09115579833052 39.0 35.0 41.0 24.0 41.0 16 35.18019700279304 39.0 35.0 41.0 25.0 41.0 17 35.407363668931325 40.0 36.0 41.0 27.0 41.0 18 35.45850545451087 40.0 36.0 41.0 27.0 41.0 19 34.859060892060604 39.0 34.0 41.0 19.0 41.0 20 35.14451521271166 40.0 35.0 41.0 25.0 41.0 21 35.13601562326624 40.0 36.0 41.0 25.0 41.0 22 35.158955954943615 40.0 36.0 41.0 25.0 41.0 23 35.174339863106205 40.0 36.0 41.0 25.0 41.0 24 33.905458315236174 39.0 34.0 41.0 10.0 41.0 25 34.50639133618872 39.0 35.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.17872706381018588 1101 2 -0.19278365986027168 1101 3 -1.0659153184808758 1101 4 -0.3732664289296679 1101 5 -0.28568554641823596 1101 6 -2.267342488007195 1101 7 -0.6715508164962323 1101 8 -0.12109442463690456 1101 9 -0.14715633001446093 1101 10 -9.75704647555683 1101 11 -4.1789447295618345 1101 12 -1.5938728667779287 1101 13 -0.7408728523668131 1101 14 -0.3822781390091734 1101 15 -1.8415726355171103 1101 16 -0.3242072330877548 1101 17 -0.08035605304600324 1101 18 0.17392864053156387 1101 19 -0.4857143233159107 1101 20 0.016573772242132634 1101 21 -0.05912669918030389 1101 22 0.1295268218940251 1101 23 0.12572592531008553 1101 24 0.14654973368635638 1101 25 0.10284425333918534 1102 1 0.26392847970766553 1102 2 0.3008134152093831 1102 3 0.02288350672159467 1102 4 0.2242204230351703 1102 5 0.45868695533908976 1102 6 -0.33301886654997404 1102 7 0.35141503689070674 1102 8 1.0270520909887857 1102 9 1.0277606755509794 1102 10 0.873184609715743 1102 11 0.5307661412591571 1102 12 0.6034276971898436 1102 13 0.613810910418735 1102 14 0.4067837628915214 1102 15 1.2053554684515433 1102 16 0.8491136810457718 1102 17 0.9549713892870741 1102 18 0.582283297615497 1102 19 0.8878870667683714 1102 20 0.8643453919733446 1102 21 0.8043216470787442 1102 22 0.8277704253488238 1102 23 0.6242609082472157 1102 24 -2.7428408980921013 1102 25 -0.8969562352318263 1103 1 -0.26358205843961713 1103 2 -0.36786421839947536 1103 3 -0.005764942541027551 1103 4 0.09397309846023205 1103 5 -0.1116791017350991 1103 6 0.564021636590013 1103 7 0.20922254548013797 1103 8 0.28706884066921745 1103 9 0.0311465700929503 1103 10 0.0936516984496123 1103 11 -0.1488695415919068 1103 12 -0.018147839925077847 1103 13 -0.08351517245274209 1103 14 -0.07615569002958011 1103 15 0.2561717253851441 1103 16 0.41907955531181074 1103 17 -0.2618812947216185 1103 18 -0.16978779341042838 1103 19 -1.567346976377138 1103 20 -0.4581845521402528 1103 21 -0.14935226309007277 1103 22 0.14671264359112257 1103 23 0.14398586086325338 1103 24 1.4666356627948431 1103 25 0.5528979590319878 1104 1 -0.2485545133368845 1104 2 -0.20862516442358015 1104 3 -1.3102103447307876 1104 4 -0.4292867142732604 1104 5 -0.13183046023232237 1104 6 -1.2527258305333966 1104 7 0.02174278281240305 1104 8 0.21057004988471206 1104 9 0.2251581062097543 1104 10 0.4278987357041686 1104 11 0.31648972827863986 1104 12 0.26065728098530627 1104 13 0.23177743983234933 1104 14 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22 0.29518336289233105 1108 23 0.5284520440307361 1108 24 -0.4723961700339814 1108 25 0.19213440611191146 1109 1 0.3599698156121178 1109 2 0.40355370963598247 1109 3 -0.993560880457462 1109 4 0.2265669693862833 1109 5 0.4565271281252663 1109 6 0.518774183130418 1109 7 0.5768920442306253 1109 8 0.8500576357828145 1109 9 0.3263638853730626 1109 10 0.4784538019316358 1109 11 0.38442201461334946 1109 12 0.069500502366445 1109 13 0.02265484597770495 1109 14 0.16394296770822336 1109 15 -1.0740123944551172 1109 16 -0.07157217874672028 1109 17 -0.1661732972977532 1109 18 0.09669670470471203 1109 19 -1.2002509175821672 1109 20 -0.36059875607418235 1109 21 -0.03123666498025557 1109 22 0.12927051161087633 1109 23 0.2389075504693281 1109 24 -0.6187905996690617 1109 25 -0.3035693684662988 1110 1 0.742065079079115 1110 2 0.5998244643853283 1110 3 0.8095003378541463 1110 4 0.5478172897768516 1110 5 0.7958484854106942 1110 6 0.9488170296921012 1110 7 0.9610014795561312 1110 8 1.2996559114256634 1110 9 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0.3332878380221942 2215 2 -0.30435973227710633 2215 3 -0.046959604714249537 2215 4 0.4846221187805497 2215 5 0.7511367935527744 2215 6 1.0483391749925772 2215 7 0.6381842886591116 2215 8 -0.7003941742765392 2215 9 -0.17617237783449013 2215 10 -0.5035386353242757 2215 11 -0.4620076710366021 2215 12 -0.716836941862077 2215 13 -0.3641793261987658 2215 14 -0.2981408840289177 2215 15 -0.2246649254559756 2215 16 -0.10242268675112598 2215 17 -0.6757864772211519 2215 18 -1.1661791302229858 2215 19 -0.11089251041936166 2215 20 -3.9667525068118508 2215 21 -1.9532176754260107 2215 22 -1.4035900249229627 2215 23 -1.1007224313755728 2215 24 -1.3751082349207735 2215 25 -1.098231657361623 2216 1 -1.1613374616338525 2216 2 -1.5807937384613133 2216 3 -0.952579241305294 2216 4 -1.2016631885188964 2216 5 -1.121042965332677 2216 6 -2.4919276491744675 2216 7 -10.299593218897428 2216 8 -11.1942777166453 2216 9 -6.403468003724772 2216 10 -3.989862159626817 2216 11 -2.85945408037545 2216 12 -2.6395447902448055 2216 13 -2.3684926361386616 2216 14 -2.589439567279449 2216 15 -3.4924705746238907 2216 16 -2.6765115586546813 2216 17 -2.9725310834123064 2216 18 -2.6767183373938863 2216 19 -2.060803916457644 2216 20 -2.8383937533810055 2216 21 -5.932377248785286 2216 22 -4.157151154756548 2216 23 -3.8281865595067615 2216 24 -4.308398718887002 2216 25 -3.5481239548983616 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26356.0 3 3.0 4 210.0 5 1341.0 6 1724.0 7 1371.0 8 1144.0 9 1078.0 10 968.0 11 988.0 12 947.0 13 936.0 14 961.0 15 1049.0 16 1039.0 17 1113.0 18 1344.0 19 1600.0 20 2127.0 21 2835.0 22 3511.0 23 4288.0 24 4939.0 25 5715.0 26 6529.0 27 7099.0 28 8090.0 29 9656.0 30 11753.0 31 14422.0 32 18748.0 33 25107.0 34 34359.0 35 48487.0 36 72234.0 37 119564.0 38 187219.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18997270077956 18.67549521548587 11.952227953257633 26.182304130476926 2 14.162098836572484 20.285769165440374 42.70583809165504 22.8462939063321 3 18.768265599476777 24.793115545741585 29.478316223265356 26.960302631516285 4 11.223058789188297 17.284414789150222 39.640424269537434 31.852102152124047 5 11.961610969381688 38.9290646439438 36.893868885573355 12.215455501101156 6 30.379040364802172 38.446768500930716 19.122305762821508 12.051885371445605 7 26.847881560057047 31.419113894466726 23.89165263594694 17.841351909529283 8 23.61293502281435 38.65085295473696 21.30971875546511 16.426493266983577 9 25.926008136758426 15.456352918419961 20.632834325324474 37.98480461949714 10 13.480958059523507 29.838822216020095 36.388374394235406 20.291845330220994 11 33.960696647561974 22.602519283058655 25.54892308180139 17.887860987577984 12 22.674786397284404 25.138134248256378 33.240530372094426 18.946548982364796 13 28.668774237326833 22.74448189592575 26.04662445183609 22.540119414911327 14 19.48202876032534 23.06882501228735 28.589570814770187 28.85957541261713 15 22.11076548424227 32.890994358989 23.304922170536738 21.693317986232003 16 20.71328458450396 28.866082357194045 29.575593452520625 20.845039605781366 17 20.574040544011467 29.661086817964172 28.91051842174524 20.854354216279123 18 20.304592671511738 28.257264436918938 31.33268013647375 20.10546275509557 19 22.919196948123812 27.376033385445243 27.902120521203628 21.80264914522732 20 22.483138478515237 28.389031053103885 29.186841829891268 19.940988638489614 21 21.767502770542045 27.762337433788403 27.682273128675227 22.78788666699432 22 21.879904999571927 29.56032178382646 28.08156845833584 20.47820475826577 23 20.837039397643657 29.124829758864035 28.696432506821534 21.341698336670774 24 22.37352790091513 27.590165078036318 29.46290976199083 20.573397259057728 25 21.631382770423993 28.378684753329814 28.935807248934193 21.054125227312003 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 173.0 1 173.0 2 275.5 3 378.0 4 378.0 5 378.0 6 540.0 7 702.0 8 702.0 9 702.0 10 1115.5 11 1529.0 12 1529.0 13 1529.0 14 2480.5 15 3432.0 16 3432.0 17 3432.0 18 5916.0 19 8400.0 20 8400.0 21 8400.0 22 13300.5 23 18201.0 24 18201.0 25 18201.0 26 26124.5 27 34048.0 28 34048.0 29 34048.0 30 44292.5 31 54537.0 32 54537.0 33 54537.0 34 61833.0 35 69129.0 36 69129.0 37 69129.0 38 73414.5 39 77700.0 40 77700.0 41 77700.0 42 80336.0 43 82972.0 44 82972.0 45 82972.0 46 82736.5 47 82501.0 48 82501.0 49 82501.0 50 77497.0 51 72493.0 52 72493.0 53 72493.0 54 62183.0 55 51873.0 56 51873.0 57 51873.0 58 43410.5 59 34948.0 60 34948.0 61 34948.0 62 27569.5 63 20191.0 64 20191.0 65 20191.0 66 15391.5 67 10592.0 68 10592.0 69 10592.0 70 7563.5 71 4535.0 72 4535.0 73 4535.0 74 3120.0 75 1705.0 76 1705.0 77 1705.0 78 1145.0 79 585.0 80 585.0 81 585.0 82 377.5 83 170.0 84 170.0 85 170.0 86 108.0 87 46.0 88 46.0 89 46.0 90 28.0 91 10.0 92 10.0 93 10.0 94 6.0 95 2.0 96 2.0 97 2.0 98 2.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24284541272624094 2 0.14345633062483554 3 0.1447244528845026 4 0.0792576412291909 5 0.024252838216132416 6 0.024728384063507566 7 0.29753318517438265 8 0.2954724865024237 9 0.02155807841433993 10 0.03899475948476193 11 0.018863318612547437 12 0.020131440872214492 13 0.017753711635338763 14 0.019655895024839345 15 0.01838777276517229 16 0.02187510897925669 17 0.0209240172845064 18 0.017436681070422 19 0.025362445193341094 20 0.020289956154672873 21 0.01759519635288038 22 0.019021833895005818 23 0.020765502002048016 24 0.01933886445992258 25 0.017912226917797147 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 630854.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.4206425859402 #Duplication Level Percentage of deduplicated Percentage of total 1 94.35020243835937 81.53805122836559 2 4.374457530972911 7.560868615831687 3 0.6092192699688178 1.5794736235932785 4 0.20180073745899507 0.6975899762209187 5 0.12255186792358203 0.5295505588031615 6 0.06660252908272585 0.34535000167067625 7 0.043803500104368266 0.2649868638572963 8 0.03604489419304564 0.2492018334484183 9 0.02403799961507085 0.18696414358935057 >10 0.14206941868017592 2.3825759883373108 >50 0.014880561962626774 0.9036817435006776 >100 0.012675876485476153 2.286489470114584 >500 7.348334190316287E-4 0.43167371667042453 >1k 9.18541773789536E-4 1.0435422359965796 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1733 0.2747069845003757 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1390 0.22033624261715073 No Hit CTGTAGGACGTGGAATATGGCAAGA 1336 0.2117764173643981 No Hit GTCCTACAGTGTGCATTTCTCATTT 1111 0.17611047881126218 No Hit CTTTAGGACGTGAAATATGGCGAGG 1003 0.15899082830575695 No Hit CTGTAGGACCTGGAATATGGCGAGA 807 0.12792183294391413 No Hit GTATCAACGCAGAGTACTTTTTTTT 783 0.12411746616491297 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.5851528245838182E-4 0.0 5 0.0 0.0 0.0 1.5851528245838182E-4 0.0 6 0.0 0.0 0.0 1.5851528245838182E-4 0.0 7 0.0 0.0 0.0 1.5851528245838182E-4 0.0 8 0.0 0.0 0.0 1.5851528245838182E-4 0.0 9 0.0 0.0 0.0 1.5851528245838182E-4 0.0 10 0.0 0.0 0.0 1.5851528245838182E-4 0.0 11 0.0 0.0 0.0 1.5851528245838182E-4 0.0 12 0.0 0.0 0.0 1.5851528245838182E-4 0.0 13 0.0 0.0 0.0 1.5851528245838182E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAATACT 60 2.5093874E-5 14.282249 4 AAAGGCG 55 1.9291515E-4 13.84174 5 CGAAATC 55 1.9885635E-4 13.792344 13 AAGGCGT 50 0.0014787104 13.322675 6 TCTAGGA 60 3.9525836E-4 12.716599 2 GTAGTAC 90 5.141701E-7 12.715584 3 ACGAAAT 60 4.1510523E-4 12.642982 12 TTAGGAC 430 0.0 12.641657 3 GACGTGA 350 0.0 12.507 7 ACGTGAA 365 0.0 12.243013 8 CTTTAGG 465 0.0 12.109007 1 AGGACGT 710 0.0 12.062784 5 TTTAGGA 475 0.0 12.047304 2 TAGGACC 325 0.0 12.011739 4 GGACGTG 685 0.0 11.947341 6 CGTGAAA 395 0.0 11.762774 9 GTACTAA 65 7.710158E-4 11.745894 1 TAAGACT 65 7.8612316E-4 11.71877 4 CTACACT 115 6.825212E-8 11.591392 4 GGCGAGG 315 0.0 11.438889 19 >>END_MODULE