FastQCFastQC Report
Thu 26 May 2016
SRR1926743_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926743_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725530
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10510.14485961986409934No Hit
GTCCTACAGTGTGCATTTCTCATTT8710.1200501702203906No Hit
GTATCAACGCAGAGTACTTTTTTTT7900.10888591788072169No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGT250.0058750919.0852581
TTCGCAG400.00533455814.2261110
GATATAC1650.013.3018471
GTCCTAC5100.012.9106161
GTCTAAG905.106576E-712.7235051
TAGGTTG751.4482657E-512.6900525
TTAGACC550.00301269912.11910254
GGCGAGG1950.011.67109619
GTAGGAC4100.011.6236443
CCTACAC1406.4028427E-1011.5738293
TAGCACA752.0265707E-411.4265814
AATCCCG1001.9672189E-611.3793219
TAGGACT1104.8065885E-711.2534524
GTCCTAG855.0765484E-511.2266231
TCCTACA5950.011.2133982
GTCTTAT600.005678043211.1330671
GTAGCAC600.005728098611.1199543
TATAACT1201.2305645E-711.1091774
TCCAACG600.005950412711.06322718
TGTAGGA4550.010.8930172
Produced by FastQC (version 0.11.5)