##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926743_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 725530 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.627867903463674 31.0 30.0 33.0 22.0 34.0 2 28.671201742174688 31.0 30.0 31.0 23.0 34.0 3 28.384565765716097 31.0 28.0 33.0 16.0 34.0 4 32.27307209901727 35.0 33.0 37.0 22.0 37.0 5 32.18282496933276 35.0 33.0 37.0 22.0 37.0 6 31.713493584000663 35.0 32.0 37.0 17.0 37.0 7 31.727032651992335 35.0 32.0 37.0 17.0 37.0 8 31.547857428362715 35.0 32.0 37.0 17.0 37.0 9 33.75397157939713 38.0 34.0 39.0 22.0 39.0 10 33.88841812192466 38.0 34.0 39.0 23.0 39.0 11 34.03866552726972 38.0 35.0 39.0 25.0 39.0 12 33.90230038730307 38.0 35.0 39.0 23.0 39.0 13 34.01544663900872 38.0 35.0 39.0 25.0 39.0 14 35.09159373147906 39.0 36.0 41.0 25.0 41.0 15 34.666095130456355 39.0 34.0 41.0 18.0 41.0 16 34.75021432607887 39.0 34.0 41.0 20.0 41.0 17 34.98056593111243 39.0 35.0 41.0 24.0 41.0 18 35.03565117913801 40.0 36.0 41.0 24.0 41.0 19 34.41410417212245 39.0 34.0 41.0 18.0 41.0 20 34.69021680702383 39.0 34.0 41.0 18.0 41.0 21 34.6848248866346 39.0 35.0 41.0 18.0 41.0 22 34.70388543547476 39.0 35.0 41.0 18.0 41.0 23 34.70476479263435 39.0 35.0 41.0 18.0 41.0 24 33.424455225834905 39.0 33.0 41.0 2.0 41.0 25 34.01860433062726 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2720520338830319 1101 2 0.2000953687714997 1101 3 -0.8531476180723381 1101 4 -0.0952645938118124 1101 5 0.15734120279881836 1101 6 -1.7749265628440902 1101 7 3.6039302146306795E-4 1101 8 0.11326757039705981 1101 9 0.2438525340103581 1101 10 -9.271805673929805 1101 11 -3.711881158495494 1101 12 -1.0117228538978296 1101 13 -0.30501517174084825 1101 14 0.0019056760141822338 1101 15 -1.4523323198905587 1101 16 0.27566667784305565 1101 17 0.27216101567643136 1101 18 0.4335672449776382 1101 19 -0.42679210885578556 1101 20 0.30343711671961415 1101 21 0.2470540606199947 1101 22 0.5192874078326213 1101 23 0.5414114907672385 1101 24 0.4956969120739245 1101 25 0.7079453157211688 1102 1 -0.02408958412393858 1102 2 -0.03210910726579286 1102 3 -0.27994296887734293 1102 4 -0.10252561387132886 1102 5 -0.06051796108155827 1102 6 -0.7265887875099608 1102 7 0.13703626501329325 1102 8 0.6298005605092243 1102 9 0.4517863699175564 1102 10 0.42860472767110025 1102 11 0.11544848199699231 1102 12 0.3309856895602934 1102 13 0.17360610023634138 1102 14 -0.029639372657406682 1102 15 0.3461969107358627 1102 16 0.5028451177457285 1102 17 0.3038854700065343 1102 18 0.16386073835391102 1102 19 0.6453334286917709 1102 20 0.34156394313169613 1102 21 0.31012005869562387 1102 22 0.2550317985049517 1102 23 -0.06884979884704734 1102 24 -3.659731760342055 1102 25 -1.8223046204604003 1103 1 0.5893551560479686 1103 2 0.09065541125595544 1103 3 0.3582550327806686 1103 4 0.4569359682221332 1103 5 0.270475537762195 1103 6 0.9635338181883704 1103 7 0.7249318974579921 1103 8 0.7201500521973401 1103 9 0.7000869065901867 1103 10 0.7551896572055483 1103 11 0.7149964552942549 1103 12 0.42746197561116617 1103 13 0.4705536110352426 1103 14 0.5659956899543985 1103 15 1.0788039180223947 1103 16 0.6437369542132885 1103 17 0.4891928568907815 1103 18 0.4623938331660753 1103 19 -0.7971946048175482 1103 20 0.2582548982344477 1103 21 0.40925440138073554 1103 22 0.42634381037283475 1103 23 0.5300052995429354 1103 24 1.8036977086574666 1103 25 1.0177180681385778 1104 1 -1.0369482931051124 1104 2 -0.7210458458723643 1104 3 -1.9474251520786723 1104 4 -1.0345566861629791 1104 5 -0.7807288038883939 1104 6 -1.9703509839146172 1104 7 -0.7422687130866237 1104 8 -0.2120006336053777 1104 9 -0.3332216940330781 1104 10 -0.25422290559787086 1104 11 -0.4133739417717379 1104 12 -0.5786595821255816 1104 13 -0.6806655702413877 1104 14 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23 0.645037199110746 1108 24 -0.2795992660286828 1108 25 0.4068557507893331 1109 1 0.6426564972562865 1109 2 0.7257868660773319 1109 3 -0.5392733704841675 1109 4 0.5880134465864302 1109 5 0.9060335542504419 1109 6 1.0830293269862032 1109 7 1.2097553255372304 1109 8 1.5432385227276697 1109 9 1.0146679327991137 1109 10 1.2389475014285054 1109 11 0.7298867387116701 1109 12 0.31926871036363735 1109 13 0.4430420241438142 1109 14 0.734707391689355 1109 15 -0.6947033212212759 1109 16 0.5141319339105905 1109 17 0.3566946000514406 1109 18 0.6218040296305318 1109 19 -0.9017760411819324 1109 20 0.1412022752903539 1109 21 0.4624540240377186 1109 22 0.5832610820187867 1109 23 0.7088601455295631 1109 24 -0.04992437411190309 1109 25 0.38851828141941525 1110 1 0.8954563399437703 1110 2 0.9048388447547815 1110 3 1.3263875326299193 1110 4 1.2077912274129048 1110 5 0.9455743297462789 1110 6 1.1410040197923585 1110 7 1.1793766322371866 1110 8 1.6186067666352386 1110 9 1.299668824541584 1110 10 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4 0.027178366087667882 2215 5 0.29797908574032306 2215 6 0.8778532686775158 2215 7 0.3485010688395249 2215 8 -1.0389916209345316 2215 9 -0.6105532432764136 2215 10 -0.5737599520437939 2215 11 -0.5248377831677189 2215 12 -0.9533414758619543 2215 13 -0.623190972384819 2215 14 -0.672876526065437 2215 15 -0.3577120620710801 2215 16 -0.3881437580314966 2215 17 -0.7747252463128049 2215 18 -1.7170305428628438 2215 19 -0.3579189600316539 2215 20 -4.307819240316462 2215 21 -2.5074538318207757 2215 22 -1.7092438294175025 2215 23 -1.5919142039293561 2215 24 -0.9676217607542341 2215 25 -0.8440950906026927 2216 1 -0.9938744436224773 2216 2 -1.5658318748167694 2216 3 -0.7246502587596062 2216 4 -0.9768205385374102 2216 5 -0.86656824009048 2216 6 -2.4611390650082754 2216 7 -9.629214078649564 2216 8 -11.232238817967062 2216 9 -6.072058545231709 2216 10 -3.7252807019301315 2216 11 -2.717931680121243 2216 12 -2.2275835248943956 2216 13 -2.030771333727518 2216 14 -2.2764497281634206 2216 15 -3.115759988654755 2216 16 -2.347956862960814 2216 17 -2.684559904971181 2216 18 -2.4590179932204776 2216 19 -1.576915984437811 2216 20 -2.5303336498687585 2216 21 -4.9607497180066 2216 22 -3.578996413572039 2216 23 -3.3312058003334855 2216 24 -3.736413140310816 2216 25 -3.2873884741974884 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35790.0 3 3.0 4 303.0 5 1749.0 6 2224.0 7 1647.0 8 1378.0 9 1343.0 10 1226.0 11 1217.0 12 1222.0 13 1170.0 14 1185.0 15 1207.0 16 1270.0 17 1408.0 18 1620.0 19 2053.0 20 2589.0 21 3449.0 22 4208.0 23 5093.0 24 6048.0 25 6996.0 26 8006.0 27 8598.0 28 9637.0 29 11688.0 30 13923.0 31 17063.0 32 22027.0 33 29104.0 34 39713.0 35 55848.0 36 82823.0 37 135924.0 38 204778.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.317800362204224 17.766932218632107 11.816809204573552 26.098458214590114 2 15.065961713362233 19.494001410458647 41.101344628043286 24.338692248135835 3 19.397911612000364 23.398033062002987 28.92014386304856 28.283911462948087 4 11.82105806124166 16.17006545647131 38.01523180086669 33.99364468142035 5 12.957701432744548 38.037003491909886 35.90174126781281 13.103553807532764 6 32.39140324515088 36.8178497084327 18.250458374115993 12.540288672300418 7 28.30892670269254 30.796453815410946 22.443317311081895 18.451302170814618 8 24.97805450224846 37.21541700592908 20.262018875201658 17.544509616620793 9 25.945597961991357 15.483572139495516 19.58412713291996 38.986702765593165 10 13.957223970239884 29.46408628006828 34.95944936064837 21.61924038904347 11 35.35902843830556 22.40946748824835 23.74274568187144 18.488758391574653 12 22.86036858799835 24.632728485822636 31.861674044032608 20.64522888214641 13 28.93338038575635 21.75627763870356 25.558563724787437 23.751778250752658 14 20.526644454339056 21.69272272867003 27.550270669664457 30.230362147326456 15 23.195917500875332 30.8235851995622 22.84288939923191 23.137607900330558 16 22.369756054523798 28.400431205611188 27.278993338920994 21.95081940094402 17 21.751207596276853 28.53966319643566 27.661536680047643 22.047592527239843 18 21.681689795254446 27.92399615679928 28.908919727005927 21.485394320940344 19 23.618735404322663 27.06531419134088 27.050563424163137 22.265386980173314 20 23.313211573481183 27.561526680516174 27.71440426678724 21.4108574792154 21 22.874570944129687 26.977930330976115 26.98882042374867 23.158678301145528 22 22.576638572519567 27.86820839846819 27.30163850359379 22.25351452541845 23 22.2569150072717 27.64500320506193 27.445393326578575 22.65268846108779 24 23.0428849096398 26.99929696869443 27.909929283321617 22.047888838344154 25 22.56270711682354 27.28359221213174 27.711748520889508 22.441952150155217 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 148.0 1 148.0 2 209.5 3 271.0 4 271.0 5 271.0 6 436.0 7 601.0 8 601.0 9 601.0 10 913.0 11 1225.0 12 1225.0 13 1225.0 14 2097.5 15 2970.0 16 2970.0 17 2970.0 18 5342.0 19 7714.0 20 7714.0 21 7714.0 22 11986.5 23 16259.0 24 16259.0 25 16259.0 26 23574.5 27 30890.0 28 30890.0 29 30890.0 30 40415.0 31 49940.0 32 49940.0 33 49940.0 34 58073.0 35 66206.0 36 66206.0 37 66206.0 38 73591.5 39 80977.0 40 80977.0 41 80977.0 42 86004.0 43 91031.0 44 91031.0 45 91031.0 46 94192.0 47 97353.0 48 97353.0 49 97353.0 50 94201.0 51 91049.0 52 91049.0 53 91049.0 54 81843.5 55 72638.0 56 72638.0 57 72638.0 58 62596.5 59 52555.0 60 52555.0 61 52555.0 62 42643.5 63 32732.0 64 32732.0 65 32732.0 66 25249.5 67 17767.0 68 17767.0 69 17767.0 70 13001.0 71 8235.0 72 8235.0 73 8235.0 74 5767.5 75 3300.0 76 3300.0 77 3300.0 78 2248.0 79 1196.0 80 1196.0 81 1196.0 82 762.5 83 329.0 84 329.0 85 329.0 86 220.0 87 111.0 88 111.0 89 111.0 90 68.0 91 25.0 92 25.0 93 25.0 94 14.5 95 4.0 96 4.0 97 4.0 98 4.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22452551927556408 2 0.1299739500778741 3 0.13121442256005955 4 0.06450456907364271 5 0.019158408335975083 6 0.019296238611773462 7 0.2978512260003032 8 0.2959216021391259 9 0.015988311992612296 10 0.036387192810772816 11 0.013783027579838188 12 0.015574821165217152 13 0.013507367028241424 14 0.01474783951042686 15 0.012680385373451132 16 0.01681529364740259 17 0.01681529364740259 18 0.012818215649249514 19 0.01984755971496699 20 0.015574821165217152 21 0.013783027579838188 22 0.01543699088941877 23 0.015850481716813917 24 0.013783027579838188 25 0.012956045925047895 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 725530.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.574182025042 #Duplication Level Percentage of deduplicated Percentage of total 1 93.27614387153452 81.6858200199977 2 5.145834653656861 9.0128452126023 3 0.8475647548148678 2.226743703485021 4 0.2863055352740064 1.0029189224345167 5 0.13633140412897396 0.5969555600460167 6 0.07415671751978312 0.38965283270742657 7 0.04241434591464679 0.2600081154721663 8 0.03009919442501142 0.2108729865106464 9 0.02513187786204448 0.19808132818696267 >10 0.11761757403629786 1.808362198553113 >50 0.00880723839947433 0.5268086252611062 >100 0.00849201560852144 1.3663078416804797 >500 9.435572784983433E-4 0.5698802347991827 >1k 1.5725954641639055E-4 0.144742418263398 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1051 0.14485961986409934 No Hit GTCCTACAGTGTGCATTTCTCATTT 871 0.1200501702203906 No Hit GTATCAACGCAGAGTACTTTTTTTT 790 0.10888591788072169 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCGGT 25 0.00587509 19.085258 1 TTCGCAG 40 0.005334558 14.22611 10 GATATAC 165 0.0 13.301847 1 GTCCTAC 510 0.0 12.910616 1 GTCTAAG 90 5.106576E-7 12.723505 1 TAGGTTG 75 1.4482657E-5 12.690052 5 TTAGACC 55 0.003012699 12.1191025 4 GGCGAGG 195 0.0 11.671096 19 GTAGGAC 410 0.0 11.623644 3 CCTACAC 140 6.4028427E-10 11.573829 3 TAGCACA 75 2.0265707E-4 11.426581 4 AATCCCG 100 1.9672189E-6 11.37932 19 TAGGACT 110 4.8065885E-7 11.253452 4 GTCCTAG 85 5.0765484E-5 11.226623 1 TCCTACA 595 0.0 11.213398 2 GTCTTAT 60 0.0056780432 11.133067 1 GTAGCAC 60 0.0057280986 11.119954 3 TATAACT 120 1.2305645E-7 11.109177 4 TCCAACG 60 0.0059504127 11.063227 18 TGTAGGA 455 0.0 10.893017 2 >>END_MODULE