FastQCFastQC Report
Thu 26 May 2016
SRR1926743_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926743_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725530
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13450.18538172094882363No Hit
GTCCTACAGTGGACATTTCTAAATT9630.13273055559384175No Hit
CTGTAGGACGTGGAATATGGCAAGA9110.12556338125232588No Hit
GTCCTACAGTGTGCATTTCTCATTT8440.11632875277383431No Hit
GGTATCAACGCAGAGTACTTTTTTT7780.1072319545711411No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTAGT456.632097E-414.8139394
TAGGACC2150.014.1741344
CCTCTAA551.8952956E-413.8711881
GTATTAA706.9685448E-613.6234871
GTCTAAT500.001455580413.3510191
CGTGATT655.514277E-513.13604818
TCTAAAC751.4383435E-512.698543
TTAGACT604.0039152E-412.697664
GTAGGAC5500.011.9481733
CTGTAGG6200.011.8436451
TGTAGGA6250.011.5939042
GTCCTAA2950.011.3144231
AGCTAAT600.005705543311.1258481
CCTACAC1303.1346644E-810.9891213
CGTGGAA2700.010.89349
TAAGGTA700.001459663910.8837094
GACGTGG2550.010.7907917
TCCTATA803.641494E-410.7262682
GATATAC2050.010.6994231
GTGTAAC909.136314E-510.5960471
Produced by FastQC (version 0.11.5)