FastQCFastQC Report
Thu 26 May 2016
SRR1926742_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926742_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences758481
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17640.23257009733928732No Hit
CTGTAGGACGTGGAATATGGCAAGA13780.1816789082389671No Hit
GTCCTAAAGTGTGTATTTCTCATTT13130.17310914841637431No Hit
GTCCTACAGTGTGCATTTCTCATTT11650.15359646451262457No Hit
CTTTAGGACGTGAAATATGGCGAGG9390.12380006882176349No Hit
GTATCAACGCAGAGTACTTTTTTTT8180.10784713130586No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAT602.45124E-514.3185941
TAGGACC2800.013.9406664
AGAACTG1301.9099389E-1012.4448815
CTATACT1155.136826E-912.4179844
CAGGACT2150.011.9559194
AGGACGT6650.011.7348465
GTAGGAC7650.011.7138433
TGTAGGA7750.011.6872542
AGGACTG1800.011.631495
GGACGTG6550.011.6234196
ATAGGAC1156.719165E-811.6054833
GTCCTAC8750.011.4548751
TAGGACT855.199077E-511.2005344
CTGTAGG7850.011.187351
GACGTGA3500.011.1473677
CCTTAAG600.00566473211.1366841
GCTATAA600.00566473211.1366841
GTCCTAA3900.011.0143021
TAGGACG7200.010.9749674
GTCTAAT700.001431345410.9094051
Produced by FastQC (version 0.11.5)